YOYO as a dye to track penetration of LK15 DNA complexes in spheroids: Use and limits

Journal of Fluorescence

Volumn 18, Issue 1, 2008, Pages 155-161

  Perry, Heather Ad ^a   Alhaj Saleh, Amer F ^a   Aojula, Harmesh ^a   Pluen, Alain ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Confocal microscopy; Non viral gene delivery; Penetration; Spheroid; YOYO 1

Indexed keywords

NON-VIRAL GENE DELIVERY; PENETRATION; SPHEROID; YOYO-1;

COMPLEXATION; CONFOCAL MICROSCOPY; DNA; PEPTIDES; TISSUE; TRANSPORT PROPERTIES;

DYES;

1,1' ((4,4,7,7 TETRAMETHYL) 4,7 DIAZAUNDECAMETHYLENE)BIS 4 (3 METHYL 2,3 DIHYDRO(BENZO 1,3 OXAZOLE) 2 METHYLIDENE)QUINOLINIUM; 1,1'-((4,4,7,7-TETRAMETHYL)-4,7-DIAZAUNDECAMETHYLENE)BIS-4-(3-METHYL-2,3-DIHYDRO(BENZO-1,3-OXAZOLE)-2-METHYLIDENE)QUINOLINIUM; BENZOXAZOLE DERIVATIVE; DIAGNOSTIC AGENT; DNA; FLUORESCENT DYE; HYALURONOGLUCOSAMINIDASE; LUCIFERASE; PEPTIDE FRAGMENT; QUINOLINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CELL STRAIN HCT116; CHEMISTRY; HUMAN; METABOLISM; MULTICELLULAR SPHEROID; PLASMID; SPECTROFLUOROMETRY;

BENZOXAZOLES; DNA; FLUORESCENT DYES; HCT116 CELLS; HUMANS; HYALURONOGLUCOSAMINIDASE; LUCIFERASES; PEPTIDE FRAGMENTS; PLASMIDS; QUINOLINIUM COMPOUNDS; SPECTROMETRY, FLUORESCENCE; SPHEROIDS, CELLULAR;

EID: 38149120716     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-007-0254-5     Document Type: Article

References (19)

1. Jain RK (2001) Delivery of molecular and cellular medicine to solid tumors. Adv Drug Deliv Rev 46(1-3):149-168
2. Alexandrakis G, Brown EB, Tong RT, McKee TD, Campbell RB, Boucher Y, Jain RK (2004) Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors. Nat Med 10(2):203-207
3. Kostarelos K, Emfietzoglou D, Papakostas A, Yang WH, Ballangrud A, Sgouros G (2004) Binding and interstitial penetration of liposomes within avascular tumor spheroids. Int J Cancer 112(4):713-721
4. Dufourcq J, Neri W, Henry-Toulme N (1998) Molecular assembling of DNA with amphipathic peptides. Febs Lett 421(1):7-11
5. Kopatz I, Remy JS, Behr JP (2004) A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin. J Gene Med 6(7):769-776
6. Gurrieri S, Wells KS, Johnson ID, Bustamante C (1997) Direct visualization of individual DNA molecules by fluorescence microscopy: Characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO. Anal Biochem 249(1):44-53
7. Krishnamoorthy G, Duportail G, Mely Y (2002) Structure and dynamics of condensed DNA probed by 1,1 '-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene) bis[4-[[3-methylbenz-1,3 -oxazol-2-yl]methylidine]-1,4-dihydroquinolinium] tetraiodide fluorescence. Biochemistry 41(51):15277-15287
8. Kunz-Schughart LA (1999) Multicellular tumor spheroids: intermediates between monolayer culture and in vivo tumor. Cell Biol Int 23(3):157-161
9. Pluen A, Boucher Y, Ramanujan S, McKee TD, Gohongi T, di Tomaso E, Brown EB, Izumi Y, Campbell RB, Berk DA, Jain RK (2001) Role of tumor-host interactions in interstitial diffusion of macromolecules: Cranial vs. subcutaneous tumors. Proc Natl Acad Sci USA 98(8):4628-4633
10. Atherton E, Sheppard RC (1989) Solid phase peptide synthesis: a practical approach. IRL Press, Oxford
11. Carlsson C, Larsson A, Jonsson M (1996) Influence of optical probing with YOYO on the electrophoretic behavior of the DNA molecule. Electrophoresis 17(4):642-651
12. Brekken C, Davies CD (1998) Hyaluronidase reduces the interstitial fluid pressure in solid tumours in a non-linear concentration-dependent manner. Cancer Lett 131(1):65-70
13. Murayama Y, Sano M (2005) Exchange of counterions in DNA condensation. Biopolymers 77(6):354-360
14. Wyman TB, Nicol F, Zelphati O, Scaria PV, Plank C, Szoka FC (1997) Design, synthesis, and characterization of a cationic peptide that binds to nucleic acids and permeabilizes bilayers. Biochemistry 36(10):3008-3017
15. Pouton CW, Seymour LW (2001) Key issues in non-viral gene delivery. Adv Drug Deliv Rev 46(1-3):187-203
16. Pouton CW, Lucas P, Thomas BJ, Uduehi AN, Milroy DA, Moss SH (1998) Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids. J Control Release 53(1-3):289-299
17. Niidome T, Takaji K, Urakawa M, Ohmori N, Wada A, Hirayama T, Aoyagi H (1999) Chain length of cationic alpha-helical peptide sufficient for gene delivery into cells. Bioconjug Chem 10(5):773-780
18. Tokunaga M, Hazemoto N, Yotsuyanagi T (2004) Effect of oligopeptides on gene expression: comparison of DNA/peptide and DNA/peptide/liposome complexes. Int J Pharm 269(1):71-80
19. Wartenberg M, Acker H (1995) Quantitative recording of vitality patterns in living multicellular spheroids by confocal microscopy. Micron 26(5):395-404