Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern

Journal of Orthopaedic Research

Volumn 26, Issue 1, 2008, Pages 56-64

  Zou, Lijin ^a,b   Zou, Xuenong ^a,d   Chen, Li ^c   Li, Haisheng ^a   Mygind, Tina ^a   Kassem, Moustapha ^c   Bünger, Cody ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

^b NANCHANG UNIVERSITY   (China)

^c ODENSE UNIVERSITY HOSPITAL   (Denmark)

^d SUN YAT SEN UNIVERSITY   (China)

Author keywords

Multilineage differentiation; Porcine bone marrow stromal cells; Real time PCR; Transcription factors

Indexed keywords

AGGRECAN; ALKALINE PHOSPHATASE; BONE SIALOPROTEIN; COLLAGEN TYPE 1; MESSENGER RNA; OSTEOCALCIN; OSTEONECTIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA 2; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR OSTERIX; TRANSCRIPTION FACTOR RUNX2; TRANSCRIPTION FACTOR SOX9;

ADIPOGENESIS; ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MARROW; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; CHONDROGENESIS; CONTROLLED STUDY; FEMALE; GENE CONTROL; GENE EXPRESSION; GENE REPRESSION; HEMATOPOIETIC STEM CELL; MARKER GENE; MESENCHYME CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STROMA CELL; SWINE;

ADIPOCYTES; ANIMALS; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL LINEAGE; CELL SEPARATION; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CHONDROCYTES; FEMALE; GENE EXPRESSION PROFILING; HEMATOPOIETIC STEM CELLS; OSTEOCYTES; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STROMAL CELLS; SUS SCROFA; TRANSCRIPTION FACTORS;

EID: 38049095046     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.20467     Document Type: Article

References (35)

1. Bianco P, Riminucci M, Gronthos S, et al. 2001. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 19:180-192.
2. Isefuku S, Joyner CJ, Reed AA, et al. 2004. Distraction osteogenesis in the Cbfa-1+/- mouse. J Orthop Res 22: 1276-1282.
3. Frendo JL, Xiao G, Fuchs S, et al. 1998. Functional hierarchy between two OSE2 elements in the control of osteocalcin gene expression in vivo. J Biol Chem 273: 30509-30516.
4. Ducy P, Starbuck M, Priemel M, et al. 1999. A Cbfa1-dependent genetic pathway controls bone formation beyond embryonic development. Genes Dev 13:1025-1036.
5. Kern B, Shen J, Starbuck M, et al. 2001. Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J Biol Chem 276:7101-7107.
6. Nakashima K, Zhou X, Kunkel G, et al. 2002. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108:17-29.
7. Milona MA, Gough JE, Edgar AJ. 2003. Expression of alternatively spliced isoforms of human Sp7 in osteoblast-like cells. BMC Genomics 4:43-53.
8. Chen CW, Tsai YH, Deng WP, et al. 2005. Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells. J Orthop Res 23:446-453.
9. Sekiya I, Tsuji K, Koopman P, et al. 2000. SOX9 enhances aggrecan gene promoter/enhancer activity and is upregulated by retinoic acid in a cartilage-derived cell line, TC6. J Biol Chem 275:10738-10744.
10. Rosen ED, Sarraf P, Troy AE, et al. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 4:611-617.
11. Jia L, Young MF, Powell J, et al. 2002. Gene expression profile of human bone marrow stromal cells: High-throughput expressed sequence tag sequencing analysis. Genomics 79:7-17.
12. Qi H, Aquiar DJ, Williams SM, et al. 2003. Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells. Proc Natl Acad Sci USA 100:3305-3310.
13. Sekiya I, Vuoristo JT, Larson BL, et al. 2002. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci USA 99:4397-4402.
14. Nakamura T, Shiojima S, Hirai Y, et al. 2003. Temporal gene expression changes during adipogenesis in human mesenchymal stem cells. Biochem Biophys Res Commun 303:306-312.
15. Caplan AI. 1991. Mesenchymal stem cells. J Orthop Res 9: 641-650.
16. Tropel P, Noel D, Platet N, et al. 2004. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp Cell Res 295:395-406.
17. Bosnakovski D, Mizuno M, Kim G, et al. 2005. Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells. Cell Tissue Res 319:243-253.
18. Izadpanah R, Joswig T, Tsien F, et al. 2005. Characterization of multipotent mesenchymal stem cells from the bone marrow of rhesus macaques. Stem Cells Dev 14:440-451.
19. Zou X, Li H, Zou L, et al. 2006. Porous tantalum trabecular metal scaffolds in combination with a novel marrow processing technique to replace autograft. Adv Exp Med Biol 585:197-207.
20. Zou X, Li H, Chen L, et al. 2004. Stimulation of porcine bone marrow stromal cells by hyaluronan, dexamethasone and rhBMP-2. Biomaterials 25:5375-5385.
21. Stiehler M, Duch M, Mygind T, et al. 2006. Optimizing viral and non-viral gene transfer methods for genetic modification of porcine mesenchymal stem cells. Adv Exp Biol Med 585:31-48.
22. Myers MA. 1998. Direct measurement of cell numbers in microtitre plate cultures using the fluorescent dye SYBR green I. J Immunol Methods 212:99-103.
23. Thomson BM, Bennett J, Dean V, et al. 1993. Preliminary characterization of porcine bone marrow stromal cells: skeletogenic potential, colony-forming activity, and response to dexamethasone, transforming growth factor beta, and basic fibroblast growth factor. J Bone Miner Res 8:1173-1183.
24. Ringe J, Kaps C, Schmitt B, et al. 2002. Porcine mesenchymal stem cells. Cell Tissue Res 307:321-327.
25. Coelho MJ, Fernande MH. 2000. Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, β- glycrophosphate and dexamethasone on osteoblastic differentiation. Biomaterials 21:1095-1102.
26. Day TF, Guo X, Garrett-Beal L, et al. 2005. Wnt/ beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis. Dev Cell 8:739-750.
27. Hill TP, Spater D, Taketo MM, et al. 2005. Canonical Wnt/ beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes. Dev Cell 8:727-738.
28. Ichida F, Nishimura R, Hata K, et al. 2004. Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation. J Biol Chem 279:34015-34022.
29. Akune T, Ohba S, Kamekura S, et al. 2004. PPARγ insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. J Clin Invest 113:846-855.
30. Herbertson A, Aubin JE. 1995. Dexamethasone alters the subpopulation make-up of rat bone marrow stromal cell cultures. JBMR 10:284-293.
31. Nuttall M, Patton AJ, Olivera DL, et al. 1998. Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders. JBMR 13:371-382.
32. Diascro DD Jr, Vogel RL, Johnson TE, et al. 1998. High fatty acid content in rabbit serum is responsible for the differentiation of osteoblasts into adipocyte-like cells. JBMR 13:96-106.
33. Justesen J, Stenderup K, Eriksen EF, et al. 2002. Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures. Calcif Tissue Int 71:36-44.
34. Lecka-Czernik B, Gubrij I, Moerman EJ, et al. 1999. Inhibition of Osf2/Cbfa1 expression and terminal osteoblast differentiation by PPARγ2. J Cell Biochem 74:357-371.
35. Hatakeyama Y, Nguyen J, Wang X, et al. 2003. Smad signaling in mesenchymal and chondroprogenitor cells. J Bone Joint Surg Am 85:13-18.