Synthesis, structure and cytotoxic activity evaluation of a dinuclear complex of Cd(II) with N′,N′2-bis[(1E)-1-(2-pyridyl)ethylidene]propanedi hydrazide

Inorganic Chemistry Communications

Volumn 11, Issue 1, 2008, Pages 47-50

  Filipović, Nenad R ^a   Bacchi, Alessia ^b   Lazić, Milan ^c   Pelizzi, Giancarlo ^b   Radulović, Siniša ^c   Sladić, Dušan M ^d   Todorović, Tamara R ^d   Andelković, Katarina K ^d  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b UNIVERSITY OF PARMA   (Italy)

^c INSTITUTE FOR ONCOLOGY AND RADIOLOGY OF SERBIA   (Serbia)

^d UNIVERSITY OF BELGRADE   (Serbia)

Author keywords

Cd(II) complex; Cytotoxic activity; Hydrazone ligand; X ray analysis

Indexed keywords

EID: 38049035877     PISSN: 13877003     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.inoche.2007.10.013     Document Type: Article

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12. 2 in the nutrient medium RPMI 1640 with HEPES (RPMI-1640 Medium, Sigma, Cat. No. R-4130) supplemented with 10%, heat-inactivated, fetal calf serum (Foetal Bovine Serum Standard Quality, PAA, Cat. No. A15-101), l-glutamine (3 mM), penicillin (192 U/mL), streptomycin (200 μg/mL). Exponentially growing cells were harvested with 0.5% trypsin (Institute for Immunology and Virusology Torlak, Belgrade). Evaluation of cytotoxic activity: For the evaluation of cytotoxic effect 100 μL of cell suspensions was seeded in triplicate in 96-well flat bottom microtiter plates (Nunc, Denmark) in density of 1200 (cells/well), and after an initial incubation period of 24 h was treated by tested compounds. Control wells were not treated with agents. Stock solutions of the complex were prepared in DMSO and afterwards diluted by growth medium. Diluted solution (50 μL) was added into the plate wells to appropriate final concentrations. After the continuous drug exposure times of 48 h and 72 h, the survival was determined by sulforhodamine B (SRB) assay (Sigma Chemicals Co., St. Louis, USA) which was performed as previously reported [13,14]. Absorbance of dissolved SRB in unbuffered 10 mM tris base was read in a microtiter plate reader at λ = 570 nm. The results of the measurements are expressed as the percentage of control growth PG (%) and were calculated according to Eq. (1). The cytotoxic activity was determined from Eq. (2)(1)PG (%) = frac(mean OD (optical density) of treated cells, mean OD of control cells) × 100 %(2)IC (%) = 100 % - PG (%).
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