Oxidative stress in primary culture hepatocytes isolated from partially hepatectomized rats

Canadian Journal of Physiology and Pharmacology

Volumn 85, Issue 10, 2007, Pages 1047-1051

  Francés, Daniel ^a   Ronco, M Teresa ^a   Ochoa, Elena ^a   Alvarez, M Luján ^a   Quiroga, Ariel ^a   Parody, J Pablo ^a   Monti, Juan ^a   Carrillo, M Cristina ^a   Carnovale, Cristina E ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

Author keywords

Catalase; Glutathione; Partial hepatectomy; Primary culture; Rat hepatocyte; Reactive oxygen species; Superoxide dismutase

Indexed keywords

CATALASE; COPPER ZINC SUPEROXIDE DISMUTASE; GLUTATHIONE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; ARTICLE; CELL ACTIVITY; CELL DIFFERENTIATION; CELL ISOLATION; CONTROLLED STUDY; ENZYME ACTIVITY; IN VITRO STUDY; LIVER CELL CULTURE; LIVER CELL DAMAGE; MALE; METABOLIC ACTIVATION; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXIDATIVE STRESS; PARTIAL HEPATECTOMY; PH MEASUREMENT; PRIORITY JOURNAL; RAT;

ANIMALS; ANTIOXIDANTS; CATALASE; CELLS, CULTURED; GLUTATHIONE; HEPATECTOMY; HEPATOCYTES; LIVER REGENERATION; MALE; MITOCHONDRIA, LIVER; OXIDATIVE STRESS; RATS; RATS, WISTAR; REACTIVE OXYGEN SPECIES; SUPEROXIDE DISMUTASE; TIME FACTORS;

EID: 38049016294     PISSN: 00084212     EISSN: None     Source Type: Journal    
DOI: 10.1139/Y07-087     Document Type: Article

References (19)

1. Baker, M.A., Cerniglia, G.J., and Zaman, A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem. 190: 360-365. doi:10.1016/0003- 2697(90)90208-Q.
2. Baur, H., Kasperek, S., and Pfaff, E. 1975. Criteria of viability of isolated liver cells. Hoppe Seylers Z. Physiol. Chem. 356: 827-838.
3. Carrillo, M.C., Kitani, K., Kanai, S., Sato, Y., Miyasaka, K., and Ivy, G.O. 1994. (-)deprenyl increases activities of superoxide dismutase and catalase in certain brain regions in old male mice. Life Sci. 54: 975-981. doi:10.1016/0024-3205(94)00499-4.
4. Donahue, J.L., Okpodu, C.M., Cramer, C.L., Grabau, E.A., and Alscher, R.G. 1997. Responses of antioxidants to paraquat in pea leaves (relationships to resistance). Plant Physiol. 113: 249-257.
5. Dreiem, A., Myhre, O., and Fonnum, F. 2002. Relationship between lipophilicity of C6-10 hydrocarbon solvents and their ROS-inducing potency in rat cerebellar granule cells. Neurotoxicology, 23: 701-709. doi:10.1016/S0161-813X(02)00010-4.
6. Fausto, N., Campbell, J.S., and Riehle, K.J. 2006. Liver regeneration. Hepatology, 43(Suppl. 1): S45-S53. doi:10.1002/hep.20969.
7. Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M.L., and Starzl, T.E. 1986. Epidermal growth factor and proliferation in rat hepatocytes in primary culture isolated at different times after partial hepatectomy. Cancer Res. 46: 1318-1323.
8. Guillouzo, A. 1998. Liver cell models in in vitro toxicology. Environ. Health Perspect. 106(Suppl. 2): 511-532. doi:10.2307/3433803.
9. Higgins, A.R., and Anderson, R.M. 1931. Experimental pathology of the liver. Restoration of liver of the white rat following partial surgical removal. Arch. Pathol. (Chic), 12: 186-202.
10. Horvath, M.E., Gonzalez-Cabello, R., Blazovics, A., van der Looij, M., Barta, I., Muzes, G., Gergely, P., and Feher, J. 2001. Effect of silibinin and vitamin E on restoration of cellular immune response after partial hepatectomy. J. Ethnopharmacol. 77: 227-232. doi:10.1016/S0378-8741(01)00298-7.
11. Jaeschke, H., Farhood, A., Cai, S.X., Tseng, B.Y., and Bajt, M.L. 2000. Protection against TNF-induced liver parenchymal cell apoptosis during endotoxemia by a novel caspase inhibitor in mice. Toxicol. Appl. Pharmacol. 169: 77-83. doi:10.1006/taap.2000.9035.
12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
13. Ronco, M.T., deAlvarez, M.L., Monti, J., Carrillo, M.C., Pisani, G., Lugano, M.C., and Carnovale, C.E. 2002. Modulation of balance between apoptosis and proliferation by lipid peroxidation (LPO) during rat liver regeneration. Mol. Med. 8: 808-817.
14. Ronco, M.T., Alvarez, M.L., Monti, J.A., Carrillo, M.C., Pisani, G.B., Lugano, M.C., and Carnovale, C.E. 2004. Role of nitric oxide increase on induced programmed cell death during early stages of rat liver regeneration. Biochim. Biophys. Acta, 1690: 70-76.
15. Seglen, P.O. 1973. Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion. Exp. Cell Res. 82: 391-398. doi:10.1016/0014-4827(73)90357-1.
16. Sies, H. 1999. Glutathione and its role in cellular functions. Free Radic. Biol. Med. 27: 916-921. doi:10.1016/S0891-5849(99)00177-X.
17. Tietze, F. 1969. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 27: 502-522. doi:10.1016/0003- 2697(69)90064-5.
18. Tzung, S.P., Fausto, N., and Hockenbery, D.M. 1997. Expression of Bcl-2 family during liver regeneration and identification of Bcl-x as a delayed early response gene. Am. J. Pathol. 150: 1985-1995.
19. Webber, E.M., Godowski, P.J., and Fausto, N. 1994. In vivo response of hepatocytes to growth factors requires an initial priming stimulus. Hepatology, 19: 489-497.