Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates

FEBS Journal

Volumn 275, Issue 2, 2008, Pages 250-262

  Larsen, Anja C V ^a   Kvissel, Anne Katrine ^a   Hafte, Tilahun T ^a   Avellan, Cecilia I A ^a   Eikvar, Sissel ^a   Rootwelt, Terje ^b   Ørstavik, Sigurd ^a,c   Skålhegg, Bjørn S ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

^c ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

C splice variants; Exon skipping; Neuronal splicing; NT2 neurones; Protein kinase A

Indexed keywords

CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; HISTONE; KEMPTIDE; MESSENGER RNA;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BRAIN REGION; CONTROLLED STUDY; COW; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; EXON; GENE EXPRESSION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIMATE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHESUS MONKEY;

BOVINAE; MACACA MULATTA; PRIMATES;

EID: 37849046923     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06195.x     Document Type: Article

References (35)

1. Skalhegg BS Tasken K (2000) Specificity in the cAMP/PKA signaling pathway. Differential expression, regulation, and subcellular localization of subunits of PKA. Front Biosci 5, D678 D693.
2. Reinton N, Orstavik S, Haugen TB, Jahnsen T, Tasken K Skalhegg BS (2000) A novel isoform of human cyclic 3′,5′-adenosine monophosphate- dependent protein kinase, c alpha-s, localizes to sperm midpiece. Biol Reprod 63, 607 611.
3. Desseyn JL, Burton KA McKnight GS (2000) Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development. Proc Natl Acad Sci USA 97, 6433 6438.
4. San Agustin JT Witman GB (2001) Differential expression of the C(s) and Calpha1 isoforms of the catalytic subunit of cyclic 3',5'-adenosine monophosphate-dependent protein kinase testicular cells. Biol Reprod 65, 151 164.
5. Orstavik S, Reinton N, Frengen E, Langeland BT, Jahnsen T Skalhegg BS (2001) Identification of novel splice variants of the human catalytic subunit Cbeta of cAMP-dependent protein kinase. Eur J Biochem 268, 5066 5073.
6. Kvissel AK, Orstavik S, Oistad P, Rootwelt T, Jahnsen T Skalhegg BS (2004) Induction of Cbeta splice variants and formation of novel forms of protein kinase A type II holoenzymes during retinoic acid-induced differentiation of human NT2 cells. Cell Signal 16, 577 587.
7. Clegg CH, Ran W, Uhler MD McKnight GS (1989) A mutation in the catalytic subunit of protein kinase A prevents myristylation but does not inhibit biological activity. J Biol Chem 264, 20140 20146.
8. Jedrzejewski PT, Girod A, Tholey A, Konig N, Thullner S, Kinzel V Bossemeyer D (1998) A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry. Protein Sci 7, 457 469.
9. Toner-Webb J, van Patten SM, Walsh DA Taylor SS (1992) Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 267, 25174 25180.
10. Yonemoto W, McGlone ML Taylor SS (1993) N-myristylation of the catalytic subunit of cAMP-dependent protein kinase conveys structural stability. J Biol Chem 268, 2348 2352.
11. Herberg FW, Bell SM Taylor SS (1993) Expression of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli: multiple isozymes reflect different phosphorylation states. Protein Eng 6, 771 777.
12. Gamm DM, Baude EJ Uhler MD (1996) The major catalytic subunit isoforms of cAMP-dependent protein kinase have distinct biochemical properties in vitro and in vivo. J Biol Chem 271, 15736 15742.
13. Skalhegg BS, Huang Y, Su T, Idzerda RL, McKnight GS Burton KA (2002) Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction. Mol Endocrinol 16, 630 639.
14. Nolan MA, Babcock DF, Wennemuth G, Brown W, Burton KA McKnight GS (2004) Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility. Proc Natl Acad Sci USA 101, 13483 13488.
15. Sastri M, Barraclough DM, Carmichael PT Taylor SS (2005) A-kinase-interacting protein localizes protein kinase A in the nucleus. Proc Natl Acad Sci USA 102, 349 354.
16. San Agustin JT, Leszyk JD, Nuwaysir LM Witman GB (1998) The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence. J Biol Chem 273, 24874 24883.
17. Orstavik S, Funderud A, Hafte TT, Eikvar S, Jahnsen T Skalhegg BS (2005) Identification and characterization of novel PKA holoenzymes in human T lymphocytes. FEBS J 272, 1559 1567.
18. Funderud A, Henanger HH, Hafte TT, Amieux PS, Orstavik S Skalhegg BS (2006) Identification, cloning and characterization of a novel 47 kDa murine PKA C subunit homologous to human and bovine Cbeta2. BMC Biochem 7, 20.
19. Pleasure SJ, Page C Lee VM (1992) Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J Neurosci 12, 1802 1815.
20. Cadd G McKnight GS (1989) Distinct patterns of cAMP-dependent protein kinase gene expression in mouse brain. Neuron 3, 71 79.
21. Herberg FW, Taylor SS Dostmann WR (1996) Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase. Biochemistry 35, 2934 2942.
22. International Human Genome Sequencing Consortium (2004) Finishing the euchromatic sequence of the human genome. Nature 431, 931 945.
23. Grabowski PJ Black DL (2001) Alternative RNA splicing in the nervous system. Prog Neurobiol 65, 289 308.
24. Yeo G, Holste D, Kreiman G Burge CB (2004) Variation in alternative splicing across human tissues. Genome Biol 5, R74.
25. Uhler MD, Chrivia JC McKnight GS (1986) Evidence for a second isoform of the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 261, 15360 15363.
26. Guthrie CR, Skalhegg BS McKnight GS (1997) Two novel brain-specific splice variants of the murine Cbeta gene of cAMP-dependent protein kinase. J Biol Chem 272, 29560 29565.
27. Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS Sowadski JM (1991) Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253, 407 414.
28. Kemp BE, Graves DJ, Benjamini E Krebs EG (1977) Role of multiple basic residues in determining the substrate specificity of cyclic AMP-dependent protein kinase. J Biol Chem 252, 4888 4894.
29. Whitehouse S, Feramisco JR, Casnellie JE, Krebs EG Walsh DA (1983) Studies on the kinetic mechanism of the catalytic subunit of the cAMP-dependent protein kinase. J Biol Chem 258, 3693 3701.
30. Zhang W, Morris GZ Beebe SJ (2004) Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells. Protein Expr Purif 35, 156 169.
31. Cadd GG, Uhler MD McKnight GS (1990) Holoenzymes of cAMP-dependent protein kinase containing the neural form of type I regulatory subunit have an increased sensitivity to cyclic nucleotides. J Biol Chem 265, 19502 19506.
32. Foss KB, Landmark B, Skalhegg BS, Tasken K, Jellum E, Hansson V Jahnsen T (1994) Characterization of in-vitro-translated human regulatory and catalytic subunits of cAMP-dependent protein kinases. Eur J Biochem 220, 217 223.
33. Roskoski R Jr. (1983) Assays of protein kinase. Methods Enzymol 99, 3 6.
34. Skalhegg BS, Johansen AK, Levy FO, Andersson KB, Aandahl EM, Blomhoff HK, Hansson V Tasken K (1998) Isozymes of cyclic AMP-dependent protein kinases (PKA) in human lymphoid cell lines: levels of endogenous cAMP influence levels of PKA subunits and growth in lymphoid cell lines. J Cell Physiol 177, 85 93.
35. Skalhegg BS, Landmark BF, Doskeland SO, Hansson V, Lea T Jahnsen T (1992) Cyclic AMP-dependent protein kinase type I mediates the inhibitory effects of 3',5'-cyclic adenosine monophosphate on cell replication in human T lymphocytes. J Biol Chem 267, 15707 15714.