The contribution of amino acid residues 1508-1515 of factor V to light chain generation

Journal of Thrombosis and Haemostasis

Volumn 6, Issue 1, 2008, Pages 118-124

  Erdogan, E ^a   Bukys, M A ^a   Kalafatis, M ^a,b  

^a CLEVELAND STATE UNIVERSITY   (United States)

^b LERNER RESEARCH INSTITUTE   (United States)

Author keywords

Activation; Coagulation; Factor Va; Mutation; Prothrombinase; Thrombin

Indexed keywords

AMINO ACID; ARGININE; BLOOD CLOTTING FACTOR 5; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; THROMBIN;

ARTICLE; BIOASSAY; CONTROLLED STUDY; GENE MUTATION; HEAVY CHAIN; HUMAN; IMMUNOBLOTTING; INCUBATION TIME; LIGHT CHAIN; MOLECULAR DYNAMICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMINO ACID SEQUENCE; BINDING SITES; BLOOD COAGULATION; FACTOR V; HUMANS; HYDROLYSIS; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; THROMBIN;

EID: 37549067747     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2007.02803.x     Document Type: Article

References (32)

1. Mann KG, Kalafatis M. Factor V: A combination of Dr Jekyll and Mr Hyde. Blood 2003; 101: 20-30.
2. Nesheim ME, Taswell JB, Mann KG. The contribution of bovine factor V and factor Va to the activity of prothrombinase. J Biol Chem 1979; 254: 10952-62.
3. Jenny RJ, Pittman DD, Toole JJ, Kriz RW, Aldape RA, Hewick RM, Kaufman RJ, Mann KG. Complete cDNA and derived amino acid sequence of human factor V. Proc Natl Acad Sci USA 1987; 84: 4846-50.
4. Suzuki K, Dahlbäck B, Stenflo J. Thrombin-catalyzed activation of human coagulation factor V. J Biol Chem 1982; 257: 6556-64.
5. van't Veer C, Golden NJ, Kalafatis M, Mann KG. Inhibitory mechanism of the protein C pathway on tissue factor-induced thrombin generation. Synergistic effect in combination with tissue factor pathway inhibitor. J Biol Chem 1997; 272: 7983-94.
6. Kalafatis M, Rand MD, Mann KG. The mechanism of inactivation of human factor V and human factor Va by Activated Protein C. J Biol Chem 1994; 269: 31869-80.
7. Guinto ER, Esmon CT. Loss of prothrombin and of factor Xa-factor Va interactions upon inactivation of factor Va by Activated Protein C. J Biol Chem 1984; 259: 13986-92.
8. Mann KG, Hockin MF, Begin KJ, Kalafatis M. Activated Protein C cleavage of factor Va leads to dissociation of the A2 domain. J Biol Chem 1997; 272: 20678-83.
9. Adams TE, Hockin MF, Mann KG, Everse SJ. The crystal structure of Activated Protein C-inactivated bovine factor Va: Implications for cofactor function. Proc Natl Acad Sci USA 2004; 101: 8918-23.
10. Orban T, Kalafatis M, Gogonea V. Completed three-dimensional model of human coagulation factor Va. Molecular dynamics simulations and structural analyses. Biochemistry 2005; 44: 13082-90.
11. Hortin GL. Sulfation of tyrosine residues in coagulation factor V. Blood 1990; 76: 946-52.
12. Marquette KA, Pittman DD, Kaufman RJ. The factor V B-domain provides two functions to facilitate thrombin cleavage and release of the light chain. Blood 1995; 86: 3026-34.
13. Bukys MA, Orban T, Kim PY, Beck DO, Nesheim ME, Kalafatis M. The structural integrity of anion binding exosite-I of thrombin is required and sufficient for timely cleavage and activation of factor V and factor VIII. J Biol Chem 2006; 280: 27393-401.
14. Pittman DD, Marquette KA, Kaufman RJ. Role of the B domain for factor VIII and factor V expression and function. Blood 1994; 84: 4214-25.
15. Nesheim ME, Katzmann JA, Tracy PB, Mann KG. Factor V. Methods Enzymol 1980; 80: 243-75.
16. Kalafatis M, Beck DO, Mann KG. Structural requirements for expression of factor Va activity. J Biol Chem 2003; 278: 33550-61.
17. Beck DO, Bukys MA, Singh LS, Szabo KA, Kalafatis M. The contribution of amino acid region ASP695-TYR698 of factor V to procofactor activation and factor Va function. J Biol Chem 2004; 279: 3084-95.
18. Barenholz Y, Gibbs D, Litmann BJ, Goll J, Thompson T, Carlson D. A simple method for the preparation of homogeneous phospholipid vesicles. Biochemistry 1977; 16: 2806-910.
19. Singh LS, Bukys MA, Beck DO, Kalafatis M. Amino acids Glu323, Tyr324, Glu330, and Val331 of factor Va heavy chain are essential for expression of cofactor activity. J Biol Chem 2003; 278: 28335-45.
20. Kalafatis M, Haley PE, Lu D, Bertina RM, Long GL, Mann KG. Proteolytic events that regulate factor V activity in whole plasma from normal and activated protein C (APC)-resistant individuals during clotting: An insight into the APC-resistance assay. Blood 1996; 87: 4695-707.
21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
22. Towbin H, Staehlin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4.
23. Hockin MF, Kalafatis M, Shatos M, Mann KG. Protein C activation and factor Va inactivation on human umbilical vein endothelial cells. Arterioscler Thromb Vasc Biol 1997; 17: 2765-75.
24. Guinto ER, Esmon CT, Mann KG, MacGillivray RT. The complete cDNA sequence of bovine coagulation factor V. J Biol Chem 1992; 267: 2971-8.
25. Grimm DR, Colter MB, Braunschweig M, Alexander LJ, Neame PJ, Kim HK. Porcine factor V: CDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains. Cell Mol Life Sci 2001; 58: 148-59.
26. Yang TL, Cui J, Rehumtulla A, Yang A, Moussalli M, Kaufman RJ, Ginsburg D. The structure and function of murine factor V and its inactivation by protein C. Blood 1998; 91: 4593-9.
27. Camire RM, Kalafatis M, Tracy PB. Proteolysis of factor V by cathepsin G and elastase indicates that cleavage at Arg1545 optimizes cofactor function by facilitating factor Xa binding. Biochemistry 1998; 37: 11896-906.
28. Gerads I, Tans G, Yukelson LY, Zwaal RF, Rosing J. Activation of bovine factor V by an activator purified from the venom of Naja naja oxiana. Toxicon 1992; 30: 1065-79.
29. Bakker HM, Guido T, Thomassen MCLGD, Yukelson LY, Ebberink R, Hemker HC, Rosing J. Functional properties of human factor Va lacking Asp683-Arg709 domain of the heavy chain. J Biol Chem 1994; 269: 20662-7.
30. Toso R, Camire RM. Removal of B-domain sequences from factor V rather than specific proteolysis underlies the mechanism by which cofactor function is realized. J Biol Chem 2004; 279: 21643-50.
31. Zhu H, Toso R, Camire RM. Inhibitory sequences within the B-domain stabilize circulating factor V in an inactive state. J Biol Chem 2007; 282: 15033-9.
32. Sauter NK, Mau T, Rader SD, Agard DA. Structure of alpha-lytic protease complexed with its pro region. Nat Struct Biol 1998; 5: 945-50.