Doxazosin induces apoptosis of cells expressing hERG K+ channels

European Journal of Pharmacology

Volumn 579, Issue 1-3, 2008, Pages 98-103

  Thomas, Dierk ^a   Bloehs, Ramona ^a   Koschny, Ronald ^a,b   Ficker, Eckhard ^c   Sykora, Jaromir ^a,b   Kiehn, Johann ^a   Schlömer, Kathrin ^a   Gierten, Jakob ^a   Kathöfer, Sven ^a   Zitron, Edgar ^a   Scholz, Eberhard P ^a   Kiesecker, Claudia ^a   Katus, Hugo A ^a   Karle, Christoph A ^a  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^c CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

Antihypertensive drug; Apoptosis; Congestive heart failure; Doxazosin; hERG channel; Potassium channel

Indexed keywords

DOXAZOSIN; MEMBRANE RECEPTOR; POTASSIUM CHANNEL HERG;

APOPTOSIS; ARTICLE; CELL DEATH; CELL STRAIN HEK293; CONTROLLED STUDY; DRUG RECEPTOR BINDING; EMBRYO; HEART FAILURE; HUMAN; HUMAN CELL; HUMAN TISSUE; KIDNEY CELL; NUCLEOTIDE SEQUENCE; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ADRENERGIC ALPHA-ANTAGONISTS; ANTIHYPERTENSIVE AGENTS; APOPTOSIS; CELL LINE; DOXAZOSIN; ELECTROPHYSIOLOGY; ETHER-A-GO-GO POTASSIUM CHANNELS; FLOW CYTOMETRY; HUMANS; IN SITU NICK-END LABELING; STAINING AND LABELING;

EID: 37549018992     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2007.10.051     Document Type: Article

References (16)

1. Eiras S., Fernandez P., Pineiro R., Iglesias M.J., Gonzalez-Juanatey J.R., and Lago F. Doxazosin induces activation of GADD153 and cleavage of focal adhesion kinase in cardiomyocytes en route to apoptosis. Cardiovasc. Res. 71 (2006) 118-128
2. Gavrieli Y., Sherman Y., and Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119 (1992) 493-501
3. Gonzalez-Juanatey J.R., Iglesias M.J., Alcaide C., Pineiro R., and Lago F. Doxazosin induces apoptosis in cardiomyocytes cultured in vitro by a mechanism that is independent of α1-adrenergic blockade. Circulation 107 (2003) 127-131
4. Lang F., Foller M., Lang K.S., Lang P.A., Ritter M., Gulbins E., Vereninov A., and Huber S.M. Ion channels in cell proliferation and apoptotic cell death. J. Membr. Biol. 205 (2005) 147-157
5. Latt S.A., and Stetten G. Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24 (1976) 24-33
6. Mitcheson J.S., Chen J., Lin M., Culberson C., and Sanguinetti M.C. A structural basis for drug-induced long QT syndrome. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 12329-12333
7. Nicoletti I., Migliorati G., Pagliacci M.C., Grignani F., and Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139 (1991) 271-279
8. Saraste A., and Pulkki K. Morphologic and biochemical hallmarks of apoptosis. Cardiovasc. Res. 45 (2000) 528-537
9. + channels in neoplastic hematopoietic cells. J. Biol. Chem. 277 (2002) 18528-18534
10. The ALLHAT Officers and Coordinators for the ALLHAT Collaborative Research Group. Major cardiovascular events in hypertensive patients randomized to doxazosin vs chlorthalidone: the Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT). JAMA 283 (2000) 1967-1975
11. Theroux T.L., Esbenshade T.A., Peavy R.D., and Minneman K.P. Coupling efficiencies of human α1-adrenergic receptor subtypes: titration of receptor density and responsiveness with inducible and repressible expression vectors. Mol. Pharmacol. 50 (1996) 1376-1387
12. Thomas D., Wendt-Nordahl G., Röckl K., Ficker E., Brown A.M., and Kiehn J. High affinity blockade of HERG human cardiac potassion channels by the novel antiarrhythmic drug BRL-32872. J. Pharmacol. Exp. Ther. 297 (2001) 735-761
13. Thomas D., Wimmer A.B., Wu K., Hammerling B.C., Ficker E.K., Kuryshev Y., Kiehn J., Katus H.A., Schoels W., and Karle C.A. Inhibition of human ether-a-go-go-related gene potassium channels by the α1-adrenoceptor antagonists prazosin, doxazosin, and terazosin. Naunyn-Schmiedeberg's Arch. Pharmacol. 369 (2004) 462-472
14. + channels in regulating tumor cell proliferation and apoptosis. Pflugers Arch. - Eur. J. Physiol. 448 (2004) 274-286
15. + channel, a regulator of tumor cell apoptosis and proliferation. Cancer Res. 62 (2002) 4843-4848
16. Wonderlin W.F., and Strobel J.S. Potassium channnels, proliferation and G1 progression. J. Membr. Biol. 154 (1996) 91-107