Real-time PCR for the quantitation of Tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci

Journal of Virological Methods

Volumn 147, Issue 2, 2008, Pages 282-289

  Mason, Giovanna ^a   Caciagli, Piero ^a   Accotto, Gian Paolo ^a   Noris, Emanuela ^a  

^a INSTITUTE FOR SUSTAINABLE PLANT PROTECTION   (Italy)

Author keywords

Bemisia tabaci; Geminivirus; Real time PCR; Tomato; TYLCSV

Indexed keywords

PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA HYBRIDIZATION; DNA PROBE; FEMALE; GEMINIVIRUS; KINETICS; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PLANT TISSUE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SWEET POTATO WHITEFLY; TOMATO; TOMATO YELLOW LEAF CURL SARDINIA VIRUS; VIRUS DETECTION; VIRUS LOAD;

ANIMALS; BEGOMOVIRUS; HEMIPTERA; INSECT VECTORS; LYCOPERSICON ESCULENTUM; PLANT DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

ALEYRODIDAE; BEMISIA; BEMISIA TABACI; GEMINIVIRIDAE; HEXAPODA; LYCOPERSICON ESCULENTUM; TOMATO YELLOW LEAF CURL SARDINIA VIRUS;

EID: 37449032878     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.09.015     Document Type: Article

References (34)

1. Accotto, G.P., Noris, E., 2007. Detection methods for TYLCV and TYLCSV, in: Czosnek, H., (Ed.), Tomato Yellow Leaf Curl Virus Disease. Management, Molecular Biology, Breeding for Resistance. Springer, Dordrecht, pp. 241-249.
2. Accotto G.P., Vaira A.M., Noris E., and Vecchiati M. Using non-radioactive probes on plants: a few examples. J. Biolumin. Chemilumin. 13 (1998) 295-301
3. Accotto G.P., Navas-Castillo J., Noris E., Moriones E., and Louro D. Typing of tomato yellow leaf curl viruses in Europe. Eur. J. Plant Pathol. 106 (2000) 179-186
4. Boonham N., Smith P., Walsh K., Tame J., Morris J., Spence N., Bennison J., and Barker I. The detection of Tomato spotted wilt virus (TSWV) in individual thrips using real-time fluorescent RT-PCR (TaqMan). J. Virol. Methods 101 (2002) 37-48
5. Boonham N., Perez L.G., Mendez M.S., Peralta E.L., Blockley A., Walsh K., Barker I., and Mumford R.A. Development of a RT-PCR assay for the detection of potato spindle tuber viroid. J. Virol. Methods 116 (2004) 139-146
6. Caciagli P., and Bosco D. Quantitation over time of Tomato yellow leaf curl geminivirus DNA in its whitefly vector. Phytopathology 87 (1997) 610-613
7. Caciagli P., Bosco D., and Al-Bitar L. Relationships of the Sardinian isolate of tomato yellow leaf curl geminivirus with its whitefly vector Bemisia tabaci Genn. Eur. J. Plant Pathol. 101 (1995) 163-170
8. Davino S., Napoli C., Davino M., and Accotto G.P. Spread of Tomato yellow leaf curl virus in Sicily: partial displacement of another Geminivirus originally present. Eur. J. Plant Pathol. 114 (2006) 293-299
9. Dellaporta S.L., Wood J., and Hicks J.B. A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 4 (1983) 19-21
10. Fabre F., Kervarrec C., Mieuzet L., Riault G., Vialatte A., and Jacquot E. Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real-time RT-PCR. J. Virol. Methods 110 (2003) 51-60
11. Filion M., St-Arnaud M., and Jabaji-Hare S.H. Direct quantification of fungal DNA from soil substrate using real-time PCR. J. Microbiol. Methods 53 (2003) 67-76
12. García-Andrés S., Monci F., Navas-Castillo J., and Moriones E. Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: evidence for the presence of a new virus species of recombinant nature. Virology 350 (2006) 433-442
13. García-Andrés S., Accotto G.P., Navas-Castillo J., and Moriones E. Founder effect, plant host, and recombination shape the emergent population of begomoviruses that cause the tomato yellow leaf curl disease in the Mediterranean basin. Virology 359 (2007) 302-312
14. García-Andrés S., Miguel Tomás D., Sánchez-Campos S., Navas-Castillo J., and Moriones E. Frequent occurrence of recombinants in mixed infections of tomato yellow leaf curl disease-associated begomoviruses. Virology 365 (2007) 210-219
15. Gharsallah Chouchane S., Gorsane F., Louzir H., Nakhla M.K., Marracki M., and Fackfack H. Development of a real-time PCR for Tomato yellow leaf curl Sardinia virus. Acta Virol. 50 (2006) 101-105
16. Heid C.A., Stevens J., Livak K.J., and Williams P.M. Real-time quantitative PCR. Genome Res. 6 (1996) 986-994
17. Higuchi R., Fockler C., Dollinger G., and Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11 (1993) 1026-1030
18. Holland P.M., Abramson R.D., Watson R., and Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5′ → 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 88 (1991) 7276-7280
19. Kheyr-Pour A., Bendahmane M., Matzeit V., Accotto G.P., Crespi S., and Gronenborn B. Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Res. 19 (1991) 6763-6769
20. Korimbocus J., Coates D., Barker I., and Boonham N. Improved detection of Sugarcane yellow leaf virus using a real-time fluorescent (TaqMan) RT-PCR assay. J. Virol. Methods 103 (2002) 109-120
21. Lefeuvre P., Hoareau M., Delatte H., Reynaud B., and Lett J.-M. A multiplex PCR method discriminating between the TYLCV and TYLCV-Mld clades of Tomato yellow leaf curl virus. J. Virol. Methods 144 (2007) 165-168
22. Lett J.M., Granier M., Hippolyte I., Grondin M., Royer M., Blanc S., Reynaud B., and Peterschmitt M. Spatial and temporal distribution of Geminiviruses in leafhoppers of the Genus Cicadulina monitored by conventional and quantitative polymerase chain reaction. Phytopathology 92 (2001) 65-74
23. Lopez R., Asensio C., Guzman M.M., and Boonham N. Development of a real-time and conventional RT-PCR assays for the detection of potato yellow vein virus (PYVV). J. Virol. Methods 136 (2006) 24-29
24. Marzachí C., and Bosco D. Relative quantification of Chrysanthemum Yellows (16Sr I) phytoplasma in its plant and insect host using real-time polymerase chain reaction. Mol. Biotechnol. 30 (2005) 117-127
25. Michaelson M.J., Price H.J., Ellison J.R., and Johnston J.S. Comparison of plant DNA contents determined by feulgen microspectrophotometry and laser flow cytometry. Am. J. Bot. 78 (1991) 183-188
26. Monci F., Sánchez-Campos S., Navas-Castillo J., and Moriones E. A natural recombinant between the geminiviruses Tomato yellow leaf curl Sardinia virus and Tomato yellow leaf curl virus exhibits a novel pathogenic phenotype and is becoming prevalent in Spanish populations. Virology 303 (2002) 317-326
27. Morilla G., Krenz B., Jeske H., Bejarano E.R., and Wege C. Tête à tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in single nuclei. J. Virol. 78 (2004) 10715-10723
28. Mumford R.A., Walsh K., Barker I., and Boonham N. Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90 (2000) 448-453
29. Olmos A., Bertolini E., Gil M., and Cambra M. Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. J. Virol. Methods 128 (2005) 151-155
30. ®) assay for the detection of Grapevine leafroll associated viruses 1-5 and 9. J. Virol. Methods 141 (2007) 22-29
31. Pico B., Sifres A., and Nuez F. Quantitative detection of Cucumber vein yellowing virus in susceptible and partially resistant plants using real-time PCR. J. Virol. Methods 128 (2005) 14-20
32. Simón B., Cenis J.L., Demichelis S., Rapisarda C., Caciagli P., and Bosco D. Survey of Bemisia tabaci (Hemiptera: Aleyrodidae) biotypes in Italy with the description of a new biotype (T) from Euphorbia characias. Bull. Entomol. Res. 93 (2003) 259-264
33. Timmermans M.C.P., Prem Das O., and Messing J. Trans replication and high copy numbers of Wheat dwarf virus vectors in maize cells. Nucleic Acids Res. 20 (1992) 4047-4054
34. Yamaoka N., Morita T., Furusawa J., and Yamamoto J. Effect of temperature on the multiplication of Cauliflower mosaic virus. J. Gen. Virol. 61 (1982) 283-287