Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy

Journal of Bacteriology

Volumn 189, Issue 24, 2007, Pages 9076-9081

  Chistoserdova, Ludmila ^a,b   Crowther, Gregory J ^a   Vorholt, Julia A ^c   Skovran, Elizabeth ^a   Portais, Jean Charles ^d   Lidstrom, Mary E ^a,b  

^a University of Washington   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c ETH ZURICH   (Switzerland)

^d INSA   (France)

Author keywords

[No Author keywords available]

Indexed keywords

FORMATE DEHYDROGENASE; METHANOL; MOLYBDOPTERIN; OXIDOREDUCTASE;

ARTICLE; BACTERIAL GENOME; BACTERIUM CULTURE; CONTROLLED STUDY; ENZYME BINDING; ENZYME METABOLISM; GENE DELETION; GENE IDENTIFICATION; GENE MUTATION; GENE OVEREXPRESSION; METHYLOBACTERIUM EXTORQUENS; METHYLOTROPHIC BACTERIUM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS;

CARBON DIOXIDE; CARBON ISOTOPES; CARBON RADIOISOTOPES; DNA, BACTERIAL; FORMATE DEHYDROGENASES; FORMIC ACIDS; GENE DELETION; GENE EXPRESSION PROFILING; MAGNETIC RESONANCE SPECTROSCOPY; METHANOL; METHYLOBACTERIUM EXTORQUENS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OXIDATION-REDUCTION; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

METHYLOBACTERIUM EXTORQUENS;

EID: 37449013879     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01229-07     Document Type: Article

References (23)

1. Anthony, C. 1982. The biochemistry of methylotrophs. Academic Press, London, United Kingdom.
2. Chistoserdova, L., S. W. Chen, A. Lapidus, and M. E. Lidstrom. 2003. Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view. J. Bacteriol. 185:2980-2987.
3. Chistoserdova, L., M. Laukel, J.-C. Portais, J. A. Vorholt, and M. E. Lidstrom. 2004. Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol. J. Bacteriol. 186:22-28.
4. Ditta, G., T. Schmidhauser, F. Yakobson, P. Lu, X. Liang, D. Finlay, D. Guinley, and D. Helinski. 1985. Plasmids related to the broad host range vector, pRK290, useful for gene cloning and monitoring gene expression. Plasmid 13:149-153.
5. Guo, X., and M. E. Lidstrom. 2006. Physiological analysis of Methylobacterium extorquens AM1 grown in continuous and batch cultures. Arch. Microbiol. 186:139-149.
6. Harder, W., M. Attwood, and J. R. Quayle. 1973. Methanol assimilation by Hyphomicrobium spp. J. Gen. Microbiol. 78:155-163.
7. Kataeva, I. A., and L. A. Golovleva. 1990. Catechol 2,3-dioxygenase from Pseudomonas aeruginosa 2x. Methods Enzymol. 188:115-121.
8. Laukel, M., L. Chistoserdova, M. E. Lidstrom, and J. A. Vorholt. 2003. The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: purification and properties. Eur. J. Biochem. 270:325-333.
9. Lidstrom, M. E. 2001. Aerobic methylotrophic prokaryotes, p. 431-445. In E. Stackebrandt (ed.), The prokaryotes, 3rd ed. Springer-Verlag, New York, NY.
10. Marx, C. J., and M. E. Lidstrom. 2001. Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Microbiology 147:2065-2075.
11. Marx, C. J., and M. E. Lidstrom. 2002. Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. BioTechniques 33:1062-1067.
12. Marx, C. J., S. J. Van Dien, and M. E. Lidstrom. 2005. Flux analysis uncovers key role of functional redundancy in formaldehyde metabolism. PLoS Biol. 3:244-253.
13. Masuda, N., and G. M. Church. 2003. Regulatory network of acid resistance genes in Escherichia coli. Mol. Microbiol. 48:699-712.
14. Notton, B. A., and E. J. Hewitt. 1971. The role of tungsten in the inhibition of nitrate reductase activity in spinach (Spinacea oleracea L.) leaves. Biochem. Biophys. Res. Commun. 44:702-710.
15. Oglesby, A. G., E. R. Murphy, V. R. Lyer, and S. M. Payne. 2005. Fur regulates acid resistance in Shigella flexneri via RyhB and YdeP. Mol. Microbiol. 58:1354-1367.
16. Okubo, Y., E. Skovran, X. Guo, D. Sivam, and M. E. Lidstrom. Implementation of microarrays for Methylobacterium extorquens AM1. OMICS, in press.
17. Saito, H., and K. I. Miura. 1963. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 71:619-629.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Simon, R., U. Priefer, and A. Pühler. 1983. Vector plasmid for in vivo manipulations of Gram-negative bacteria, p. 98-106. In A. Pühler (ed.), Molecular genetics of the bacteria-plant interactions. Springer, Berlin, Germany.
20. 2 fixation by Azotobacter. Biochim. Biophys. Acta 23:433-435.
21. 13C-label tracing and mass spectrometry. Biotechnol. Bioeng. 84:45-55.
22. Vorholt, J. A., and R. K. Thauer. 2003. Molybdenum and tungsten enzymes in C1 metabolism. Met. Ions Biol. Syst. 39:571-619.
23. Whitaker, J. R., and P. E. Granum. 1980. An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal. Biochem. 109:156-159.