Wide confocal cytometry: A new approach to study proteomic and structural changes in the cell nucleus during the cell cycle

Histochemistry and Cell Biology

Volumn 129, Issue 1, 2008, Pages 45-53

  Iborra, Francisco J ^a   Buckle, Veronica ^a  

^a JOHN RADCLIFFE HOSPITAL   (United Kingdom)

Author keywords

Confocal microscopy; Cytomics; High content analysis; Nuclear structure and function

Indexed keywords

DNA; LIPID; PROTEIN; RNA;

ARTICLE; CELL CULTURE; CELL CYCLE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELL FUNCTION; CELL NUCLEUS; CELL SHAPE; CELL STRUCTURE; CONFOCAL MICROSCOPY; CYTOLOGY; GENE EXPRESSION; HELA CELL; HUMAN; HUMAN CELL; ILLUMINATION; IMAGE ANALYSIS; MITOSIS; PRIORITY JOURNAL; PROTEOMICS; RNA METABOLISM; WIDE CONFOCAL CYTOMETRY;

CELL CYCLE; CELL NUCLEUS; CELLS, CULTURED; HELA CELLS; HUMANS; MICROSCOPY, CONFOCAL; PROTEOMICS;

EID: 37449003082     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-007-0352-0     Document Type: Article

References (18)

1. Bocsi J, Mittag A, Sack U, Gerstner AO, Barten MJ, Tarnok A (2006) Novel aspects of systems biology and clinical cytomics. Cytometry A 69:105-108
2. Bubulya PA, Prasanth KV, Deerinck TJ, Gerlich D, Beaudouin J, Ellisman MH, Ellenberg J, Spector DL (2004) Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei. J Cell Biol 167:51-63
3. Calabuig J, de Juan J, Iborra FJ (2005) Specific detection of RNA on ultra-thin sections. J Struct Biol 152:146-148
4. Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Melamed MR (1999) Laser-scanning cytometry: a new instrumentation with many applications. Exp Cell Res 249:1-12
5. Iborra FJ (2002) The path that RNA takes from the nucleus to the cytoplasm: a trip with some surprises. Histochem Cell Biol 118:95-103
6. Iborra FJ (2007) Can visco-elastic phase separation, macromolecular crowding and colloidal physics explain nuclear organisation? Theor Biol Med Model 4:15
7. Iborra FJ, Pombo A, Jackson DA, Cook PR (1996) Active RNA polymerases are localized within discrete transcription "factories' in human nuclei. J cell sci 109:1427-1436
8. Iborra FJ, Jackson DA, Cook PR (2001) Coupled transcription and translation within nuclei of mammalian cells. Science 293:1139-1142
9. Iborra FJ, Escargueil AE, Kwek KY, Akoulitchev A, Cook PR (2004a) Molecular cross-talk between the transcription, translation, and nonsense-mediated decay machineries. J cell sci 117:899-906
10. Iborra FJ, Kimura H, Cook PR (2004b) The functional organization of mitochondrial genomes in human cells. BMC Biol 2:9
11. Jackson DA, Iborra FJ, Manders EM, Cook PR (1998) Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei. Mol Biol cell 9:1523-1536
12. Shackney S, Emlet DR, Pollice A, Smith C, Brown K, Kociban D (2006) Guidelines for improving the reproducibility of quantitative multiparameter immunofluorescence measurements by laser scanning cytometry on fixed cell suspensions from human solid tumors. Cytometry B Clin Cytom 70:10-19
13. Tarnok A (2006) Slide-based cytometry for cytomics-a minireview. Cytometry A 69:555-562
14. Tarnok A, Gerstner AO (2002) Clinical applications of laser scanning cytometry. Cytometry 50:133-143
15. Tarnok A, Brockhoff G (2006) Cytomics emerging from cytometry. Cell Prolif 39:335-338
16. Tarnok A, Bocsi J, Brockhoff G (2006) Cytomics-importance of multimodal analysis of cell function and proliferation in oncology. Cell Prolif 39:495-505
17. Valet GK, Tarnok A (2003) Cytomics in predictive medicine. Cytometry B Clin Cytom 53:1-3
18. Valet G, Leary JF, Tarnok A (2004) Cytomics-new technologies: towards a human cytome project. Cytometry A 59:167-171