Effects of replacing active site residues in a cold-active alkaline phosphatase with those found in its mesophilic counterpart from Escherichia coli

FEBS Journal

Volumn 275, Issue 1, 2008, Pages 117-127

  Gudjónsdóttir, Katrín ^a   Ásgeirsson, Bjarni ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

Author keywords

Cold adaptation; Metalloenzyme; Mutagenesis; Protein stability; Psychrophilic

Indexed keywords

ALKALINE PHOSPHATASE; AMINO ACID; MAGNESIUM; SODIUM CHLORIDE;

AFFINITY LABELING; ARTICLE; CATALYSIS; ESCHERICHIA COLI; KINETICS; MESOPHILIC BACTERIUM; MUTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL; RESIDUE ANALYSIS; THERMOSTABILITY; VIBRIO;

ADAPTATION, BIOLOGICAL; ALKALINE PHOSPHATASE; BINDING SITES; CATALYSIS; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; TEMPERATURE; UREA; VIBRIO; ZINC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO;

EID: 37349128850     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06182.x     Document Type: Article

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