Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy

Journal of Microscopy

Volumn 229, Issue 1, 2008, Pages 104-114

  Spitz, J A ^a,b,d   Yasukuni, R ^a   Sandeau, N ^c   Takano, M ^a   Vachon, J J ^a   Meallet Renault, R ^a   Pansu, R B ^a  

^a Ecole Normale Superieure de Cachan   (France)

^b Ecole Normale Superieure de Cachan   (France)

^c LABORATOIRE DE PHOTONIQUE QUANTIQUE ET MOLÉCULAIRE   (France)

^d UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Anisotropy; FLIM; Fluorescence; Quadrant anode; Single photon counting; Tr FAIM; Wide field imaging

Indexed keywords

ANISOTROPY; PARTICLE BEAMS; PHOTONS;

COUNTING DEVICE; FLIM; FLUORESCENCE INTENSITIES; IMAGING MICROSCOPY; QUADRANT ANODE; SINGLE PHOTON COUNTING; TIME-RESOLVED ANISOTROPY; TR-FAIM; WIDE FIELD IMAGING; WIDE-FIELD;

FLUORESCENCE;

ANISOTROPY; ANISOTROPY IMAGING MICROSCOPY; ARTICLE; CAMERA; COMPUTER; DEVICE; FLUORESCENCE SPECTROSCOPY; MICROSCOPY; POLARIZATION; PRIORITY JOURNAL; TIME;

EID: 37349079986     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2007.01873.x     Document Type: Article

References (30)

1. Agranovich, V.M. & Galanin, M.D. (1982) Electronic Excitation Energy Transfer in Condensed Matter. Elsevier, Amsterdam.
2. Axelrod, D. (1979) Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys. J. 26 (3 557 573.
3. Berberan-Santos, M.N. & Valeur, B. (1991) Fluorescence depolarization by electronic energy transfer in donor-acceptor pairs of like and unlike chromophores. J. Chem. Phys. 11, 8048 8055.
4. Buurman, E.P., Sanders, R., Draaijer, A., Gerritsen, H.C., van Veen, J.J.F., Houpt, P.M. & Levine, Y.K. (1992) Fluorescence lifetime imaging using a confocal laser scanning microscope. Scanning 14, 155 159.
5. Clayton, A.H., Hanley, Q.S., Arndt-Jovin, D.J., Subramaniam, V. & Jovin, T.M. (2002) Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM). Biophys. J. 83 (3 1631 1649.
6. Emiliani, V., Sanvitto, D., Tramier, M., et al 2003) Low-intensity two-dimensional imaging of fluorescence lifetimes in living cells. Appl. Phys. Lett. 83 (12 2471 2473.
7. Enderlein, J. (2000) Theoretical study of detecting a dipole emitter through an objective with high numerical aperture. Opt. Lett. 25, 634 636.
8. Fleming, G.R. (1986) Chemical Application of Ultrafast Spectroscopy, Oxford University press, Oxford.
9. Förster, T. (1959) Transfer mechanisms of electronic excitation. Disc. Faraday Soc. 27, 7 17.
10. Gadella, T.W.J., Jovin, T.M. & Clegg, R.M. (1993) Fluorescence lifetime imaging microscopy (FLIM): spatial resolution of microstructures on the nanosecond time scale. Biophys. Chem. 48 (1 221 239.
11. Gautier, I., Tramier, M., Durieux, C., et al 2001) Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins. Biophys. J. 80 (6 3000 3008.
12. Gee, M.L., Lensun, L., Smith, T.A. & Scholes, C.A. (2004) Time-resolved evanescent wave-induced fluorescence anisotropy for the determination of molecular conformational changes of proteins at an interface. Eur. Biophys. J. 33 (2 130 139.
13. Ha, T., Laurence, T.A., Chemla, D.S. & Weiss, S. (1999) Polarization spectroscopy of single fluorescent molecules. J. Phys. Chem. B 103 (33 6839 6850.
14. Herman, B., Wang, X.F., Wodnicki, P., Perisamy, A., Mahajan, N., Berry, G. & Gordon, G. (1999) Fluorescence lifetime imaging microscopy. Applied Fluorescence in Chemistry, Biology and Medicine (eds. by W. Rettig, B. Strehmel, S. Schrader & H. Seifert) pp. 491 507. Springer, Berlin.
15. Lakowicz, J.R. (1999) Principles of Fluorescence Spectroscopy. Kluwer Academic /Plenum Publishers, New York.
16. Magde, D., Wong, R. & Seybold, P.G. (2002) Fluorescence quantum yields and their relation to lifetimes of rhodamine 6G and fluorescein in nine solvents: improved absolute standards for quantum yields. Photochem. Photobiol. 75 (4 327 334.
17. Méallet-Renault, R., Yoshikawa, H., Tamaki, Y., Asahi, T., Pansu, R.B. & Masuhara, H. (2000) Confocal microscopic study on fluorescence quenching of single latex beads in poly(vinyl alcohol) film. Polym. Adv. Technol. 11 (8-12 772 777.
18. Millar, D.P. (1996) Time-resolved fluorescence spectroscopy. Curr. Opin. Struct. Biol. 6 (5 637 642.
19. Millar, D.P., Robbins, R.J. & Zewail, A.H. (1981) Picosecond dynamics of electronic energy transfer in condensed phases. J. Chem. Phys. 75 (8 3649 3659.
20. 2+ based on green fluorescent proteins and calmodulin. Nature 388 (6645 882 887.
21. Schaffer, J., Volkmer, A., Eggeling, C., Subramaniam, V., Striker, G. & Seidel, C.A.M. (1999) Identification of single molecules in aqueous solution by time-resolved fluorescence anisotropy. J. Phys. Chem. A 103 (3 331 336.
22. Schoutteten, L., Denjean, P., Joliff-Botrel, G., Bernard, C., Pansu, D. & Pansu, R.B. (1999) Development of intracellular calcium measurement by time-resolved photon-counting fluorescence. Photochem. Photobiol. 70 (5 701 709.
23. Schoutteten, L., Denjean, P. & Pansu, R.B. (1997) Characterisation of a time resolved photon counting confocal fluorescence microscope. J. Fluoresc. 7 (2 155 165.
24. Siegel, J., Suhling, K., Leveque-Fort, S., et al 2003) Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM): imaging the rotational mobility of a fluorophore. Rev. Sci. Instrum. 74 (1 182 192.
25. So, P.T., French, T., Yu, W., Berland, K., Dong, C.Y. & Gratton, E. (1995) Time-resolved fluorescence microscopy using two-photon excitation. Bioimaging 3, 49 63.
26. So, P.T., Konig, K., Berland, K., et al 1998) New time-resolved techniques in two-photon microscopy. Cell. Mol. Biol. (Noisy-le-grand) 44 (5 771 793.
27. Tramier, M., Kemnitz, K., Durieux, C., Coppey, J., Denjean, P., Pansu, R.B. & Coppey-Moisan, M. (2000) Restrained torsional dynamics of nuclear DNA in living proliferative mammalian cells. Biophys. J. 78 (5 2614 2627.
28. Tripathi, N. (2005) Densities, viscosities, and refractive indices of mixtures of hexane with cyclohexane, decane, hexadecane, and squalane at 298.15K. Int. J. Thermophys. 26 (3 693 703.
29. Valeur, B. (2001) Molecular Fluorescence: Principle and Applications. Wiley, New York.
30. Wang, X.F., Perisamy, A. & Herman, B. (1992) Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications. Crit. Rev. Anal. Chem. 23, 369 395.