Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites

Journal of Applied Microbiology

Volumn 92, Issue 5, 2002, Pages 912-919

  Chen, M L ^a   Tsen, Hau Yang ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ANNEALING; BACTERIOLOGY; GENES; POLYMERASE CHAIN REACTION; RNA;

16S RRNA; 16S RRNA GENE; BACILLUS SPP; BACILLUS THURINGIENSIS; GYRB GENES; PCR METHOD; PCR PRIMERS; SEQUENCE ANALYSIS; THURINGIENSIS; VARIABLE REGIONS;

BACILLUS CEREUS;

BACTERIAL RNA; DNA 16S; PRIMER DNA; RNA 16S;

BACTERIUM; POLYMERASE CHAIN REACTION;

ARTICLE; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA PROBE; GENE SEQUENCE; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SPECIES DIFFERENCE;

BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 0036231441     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2002.01606.x     Document Type: Article

References (17)

1. Genotypic diversity among Bacillus cereus and Bacillus thuringiensis strains
2. Enterotoxin-producing strains of Bacillus thuringiensis isolated from food
3. Bacillus cereus and related species
4. Discrimination between Bacillus cereus and Bacillus thuringiensis using specific DNA probes based on variable regions of 16S rRNA
5. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis - One species on the basis of genetic evidence
6. Differentiation of Bacillus anthracis and other 'Bacillus cereus group' bacteria using IS231-derived sequences
7. Insecticidal crystal proteins of Bacillus thuringiensis
8. Enterotoxigenic profiles and polymerase chain reaction detection of Bacillus cereus group cells and B. cereus strains from foods and food-borne outbreaks
9. Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-Amplified DNA
10. Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies
11. Diversity and differential distribution of IS231, IS232 and IS240 among Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides
12. Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms
13. Deoxyribonucleic acid homology and taxonomy of the genus Bacillus
14. Bacillus anthracis
15. Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice
16. A comparative study of enzyme variation in Bacillus cereus and B. thuringiensis