Immunochemical identification and partial characterization of a native hepatitis C viral non-structural 4 antigen in sera of HCV infected patients

Clinica Chimica Acta

Volumn 388, Issue 1-2, 2008, Pages 115-122

  Attallah, Abdelfattah M ^a   Ismail, Hisham ^a   Shiha, Gamal E ^b   Abou Dobara, Mohamed I ^c   El Sherbiny, Rania E ^a   El Dosoky, Ibrahim ^b  

^a Biotechnology Research Center of New Damietta   (Egypt)

^b MANSOURA UNIVERSITY   (Egypt)

^c MANSOURA UNIVERSITY   (Egypt)

Author keywords

27 kDa; Diagnosis; ELISA; Hepatitis C Virus; Liver; NS4 antigen; Serum

Indexed keywords

HEPATITIS C VIRAL NON STRUCTURAL 4 ANTIGEN; POLYPEPTIDE; UNCLASSIFIED DRUG; VIRUS ANTIGEN; VIRUS RNA;

ADOLESCENT; ADULT; ANTIGEN DETECTION; ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; CYTOPLASM; DIAGNOSTIC PROCEDURE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HEPATITIS C; HUMAN; HUMAN TISSUE; IMMUNOCHEMISTRY; IMMUNOHISTOCHEMISTRY; LIVER BIOPSY; LIVER CELL; LIVER DISEASE; MAJOR CLINICAL STUDY; MALE; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; SCHOOL CHILD; SEROTYPE; SILVER STAINING; WESTERN BLOTTING;

ADOLESCENT; ADULT; BLOTTING, WESTERN; CHILD; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HEPATITIS C; HEPATITIS C ANTIGENS; HUMANS; IMMUNOHISTOCHEMISTRY; LIVER; MALE; MIDDLE AGED; VIRAL NONSTRUCTURAL PROTEINS;

HEPATITIS C VIRUS; ORYCTOLAGUS CUNICULUS;

EID: 37249053852     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2007.10.019     Document Type: Article

References (39)

1. Heintges T., and Wands J.R. Hepatitis C virus: epidemiology and transmission. Hepatology 26 (1997) 521-526
2. Tsukuma H., Hiyama T., Tanaka S., et al. Risk factors for hepatocellular carcinoma among patients with chronic liver disease. N Engl J Med 328 (1993) 1797-1801
3. Frank C., Mohamed M.K., Strickland G.T., et al. The role of parentral anti-schistosomal therapy in the spread of hepatitis C virus in Egypt. Lancet 355 (2000) 887-891
4. Choo Q.-L., Kuo G., Weiner A.J., Overby L.R., Bradley D.W., and Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244 (1989) 359-362
5. Grakoui A., Wychowski C., Lin C., Feinstone S.M., and Rice C.M. Expression and identification of hepatitis C virus polyprotein cleavage products. J Virol 67 (1993) 1385-1395
6. Bartenschlager R., and Lohmann V. Replication of hepatitis C virus. J Gen Virol 81 (2000) 1631-1648
7. Veillon P., Payan C., Picchio G., Maniez-Montreuil M., Guntz P., and Lunel F. Comparative evaluation of the total hepatitis C virus core antigen, branched-DNA, and amplicor monitor assays in determining viremia for patients with chronic hepatitis C during interferon plus ribavirin combination therapy. J Clin Microbiol 41 (2003) 3212-3220
8. Pradat P., Maynard M., Buti M., et al. The predictive value of core antigen testing for the management of hepatitis C patients receiving pegylated interferon/ribavirin treatment. J Med Virol 73 (2004) 392-396
9. Kim J.E., Song W.K., Chung K.M., Back S.H., and Jang S.K. Subcellular localization of hepatitis C viral proteins in mammalian cells. Arch Virol 144 (1999) 329-343
10. Masalova O.V., Lakina E.I., Abdulmedzhidova A.G., et al. Characterization of monoclonal antibodies and epitope mapping of the NS4 protein of hepatitis C virus. Immunol Lett 83 (2002) 187-196
11. Konan K.V., Giddings T.H., Ikeda M., Li K., Lemon S.M., and Kirkegaard K. Nonstructural protein precursor NS4A/B from hepatitis C virus alters function and ultrastructure of host secretory apparatus. J Virol 77 (2003) 7843-7855
12. Gretton S.N., Taylor A.I., and McLauchlan J. Mobility of the hepatitis C virus NS4B protein on the endoplasmic reticulum membrane and membrane-associated foci. J Gen Virol 86 (2005) 1415-1421
13. Attallah A.M., Ismail H., Tabll A.A., Shiha G.E., and El-Dosoky I. A novel antigen detection immunoassay for field diagnosis of hepatitis C virus infection. J Immunoassay Immunochem 24 (2003) 395-407
14. Attallah A.M., Shiha G.E., Abdel-Malak C.A., Hagras H.E., Abdel-Razik W.S., and Ismail H. Utility of a novel HCV antigen detection immunoassay for monitoring treatment of HCV-infected individuals with pegylated interferon α-2a. Hepatol Res 28 (2004) 68-72
15. Laemmli U.K. Cleavage of structure of proteins during assembly of the head of the bacteriophage T4. Nature 277 (1970) 680-685
16. Lowry O.H., Rosenbrough N.J., Farr A.L., and Randall R.J. Protein measurement with Folin-Phenol reagent. J Biol Chem 193 (1951) 265-275
17. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedure and some applications. Proc Natl Acad Sci U S A 76 (1979) 4350-4354
18. Bhattacherjee N., Prescott L.E., Pike I., et al. Use of NS-4 peptides to identify type-specific antibody to hepatitis C virus genotypes 1, 2, 3, 4, 5 and 6. J Gen Virol 76 (1995) 1737-1748
19. Beld M., Penning M., van Putten M., et al. Quantitative antibody responses to structural (Core) and nonstructural (NS3, NS4, and NS5) hepatitis C virus proteins among seroconverting injecting drug users: impact of epitope variation and relationship to detection of HCV-RNA in blood. Hepatology 29 (1999) 1288-1298
20. Miyanari Y., Hijikata M., Yamaji M., Hosaka M., Takahashi H., and Shimotohno K. Hepatitis C virus non-structural proteins in the probable membranous compartment function in viral genome replication. J Biol Chem 278 (2003) 50301-50308
21. Reed K.E., and Rice C.M. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr Top Microbiol Immunol 242 (2000) 55-84
22. Ferrieu-Weisbuch C., Bettsworth F., Becquart L., et al. Usefulness of the phage display technology for the identification of a hepatitis C virus NS4A epitope recognized early in the course of the disease. J Virol Methods 131 (2006) 175-183
23. Gonzalez-Peralta R.P., Fang J.W., Gary L., et al. Significance of hepatic expression of hepatitis C viral antigens in chronic hepatitis C. Dig Dis Sci 40 (1995) 2595-2601
24. Desombere I., Van Vlierberghe H., Weiland O., et al. Serum levels of anti-NS4a and anti-NS5a predict treatment response of patients with chronic hepatitis C. J Med Virol 79 (2007) 701-713
25. Gerlach J.T., Ulsenheimer A., Gruner N.H., et al. Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins. J Virol 79 (2005) 12425-12433
26. Sansonno D., and Dammacco F. Hepatitis C virus c100 antigen in liver tissue from patients with acute and chronic infection. Hepatology 18 (1993) 240-245
27. Brody R.I., Eng S., Melamed J., et al. Immunohistochemical detection of hepatitis C antigen by monoclonal antibody TORDJI-22 compared with PCR viral detection. Am J Clin Pathol 110 (1998) 32-37
28. Lundin M., Monné M., Widell A., von Heijne G., and Persson M.A. Topology of the membrane-associated hepatitis C virus protein NS4B. J Virol 77 (2003) 5428-5438
29. Zheng Y., Ye L., Liu J., et al. Gene Expression profiles of HeLa Cells Impacted by hepatitis C virus non-structural protein NS4B. J Biochem Mol Biol 38 (2005) 151-160
30. Sansonno D., Cornacchiulo V., Racanelli V., and Dammacco R. In situ simultaneous detection of hepatitis C virus RNA and hepatitis C virus-related antigens in hepatocellular carcinoma. Cancer 80 (1997) 22-33
31. Aoyagi K., Iida K., Ohue C., et al. Performance of a conventional enzyme immunoassay for hepatitis C virus core antigen in the early phases of hepatitis C infection. Clin Lab 47 (2001) 119-127
32. Leary T.P., Gutierrez R.A., Muerhoff A.S., Birkenmeyer L.G., Desai S.M., and Dawson G.J. A chemiluminescent, magnetic particle-based immunoassay for the detection of hepatitis C virus core antigen in human serum or plasma. J Med Virol 78 (2006) 1436-1440
33. Pokriefka R.A., Manzor O., Markowitz N.P., Saravolatz L.D., Kvale P., and Donovan R.M. Increased detection of human immunodeficiency virus antigenemia after dissociation of immune complexes at low pH. J Clin Microbiol 31 (1993) 1656-1658
34. Desombere I., van Vlierberghe H., Couvent S., Clinckspoor F., and Leroux-Roels G. Comparison of qualitative (COBAS AMPLICOR HCV 2.0 versus VERSANT HCV-RNA) and quantitative (COBAS AMPLICOR HCV monitor 2.0 versus VERSANT HCV-RNA 3.0) assays for hepatitis C virus (HCV) RNA detection and quantification: impact on diagnosis and treatment of HCV infections. J Clin Microbiol 43 (2005) 2590-2597
35. Puoti C., Castellacci R., Bellis L., et al. Hepatitis C virus RNA quantitation in hepatic veins and peripheral blood in patients with liver cirrhosis: evidence for low level intrahepatic hepatitis c virus replication in advanced liver disease. Dig. Liver Dis. 34 (2002) 802-807
36. Duvoux C., Pawlotski J.M., Bastie A., Cherqui D., Soussy C.J., and Dhumeaux D. Low HCV replication levels in end-stage hepatitis C-related liver disease. J Hepatol 31 (1999) 593-597
37. McGuinness P.H., Bishop A.G., Painter D.M., Chan D., and McCaughan G.W. Intrahepatic C RNA levels do not correlate with degree of liver injury in patients with chronic hepatitis C. Hepatology 23 (1996) 676-687
38. Moldavy J., Deny P., Pol S., Bechot C., and Lamas E. Detection of hepatitis C virus in peripheral blood mononuclear cells of infected patients by in situ hybridization. Blood 83 (1994) 269-273
39. Haruna Y., Kanda T., Honda M., Takao T., and Hayashi N. Detection of hepatitis C virus in the bile and bile duct epithelial cells of hepatitis C virus-infected patients. Hepatology 33 (2001) 977-980