Immunochemical detection of central nervous tissue in retail meat products using myelin proteolipid protein (PLP) as marker

Archiv fur Lebensmittelhygiene

Volumn 58, Issue 6, 2007, Pages 214-219

  Hammon, Antje ^a   Düthorn, Tina ^a   Bäuerlein, Rainer ^b   Becker, Cord Michael ^b   Pischetsrieder, Monika ^b   Pichner, Rohtraud ^a   Gareis, Manfred ^a  

^a FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

Central nervous system (CNS); Meat products; Myelin proteolipid protein (PLP); Western blotting

Indexed keywords

EID: 37149043849     PISSN: 0003925X     EISSN: None     Source Type: Journal    
DOI: 10.2377/0003-925X-58-214     Document Type: Article

References (25)

1. Anonymous (2001): Deutsches Lebensmittelbuch. Leitsätze für Fleisch und Fleischerzeugnisse. Köln: Bundesanzeiger Verlag.
2. Aupperle, H., E. Lücker, M. Overhoff, H.A. Schoon (2002): Verfahren zum Nachweis von im Hinblick auf die BSE unerwünschten Zutaten in Fleischerzeugnissen. Fleischwirtsch. 3, 100-104.
3. Commission Directive 2001/1001/EC: Commission Directive 2001/1001/EC of November 2001 amending Directive 200/13/ EC of the European Parliament and of the Council on the approximation of the laws of the Member States relating to the labelling, presentation and advertising of foodstuffs. Off. J. Eur. Comm. L 310, 19-21.
4. Dormont, D. (2002a): Prion diseases: pathogenesis and public health concerns. FEBS Lett. 529, 17-21.
5. Dormont, D. (2002b): Prions, BSE and food. Int. J. Food Microbiol. 78, 181-189.
6. Folch, J. (1942): Brain cephalin, a mixture of phosphatides. J. Biol. Chem. 146, 35-44.
7. Hill, A.F., M. Desbruslais, S. Joiner, K.C. Sidle, I. Gowland, J. Collinge, LJ. Doey, P. Lantos (1997): The same prion strain causes vCJD and BSE. Nature 389, 448-150.
8. Kelley, L.C., S. Hafner, P.C. McCaskey, M.T. Sutton, K.A. Langheinrich (2000): An evaluation of methods for the detection of spinal cord in product derived from advanced meat recovery systems. J. Food Prot. 63, 1107-1112.
9. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
10. Lees, M.B., S.W. Brostoff (1984): Proteins of Myelin. In: P. Morell, ed. Myelin. NewYork: Plenum Press, 197-224.
11. Lücker, M., E. Eigenbrodt, S. Wenisch, K. Failing, R. Leiser, M. Bülte (1999): Development of an integrated procedure for the detection of central nervous tissue in meat products using cholesterol and neuron-specific enolase as markers. J. Food Prot. 62, 268-276.
12. Lücker, M., E. Eigenbrodt, S. Wenisch, R. Leiser, M. Bülte (2000): Identification of central nervous system tissue in retail meat products. J. Food Prot. 63, 258-263.
13. Lücker, E., S. Horlacher, E. Eigenbrodt (2001 ): Brain in human nutrition and variant Creutzfeldt-Jakob disease risk (vCJD): detection of brain in retail liver sausages using cholesterol and neuron specific enolase (NSE) as markers. Br. J. Nutr. 86, 115-119.
14. Maignien, T., C.I. Lasmezas, V. Beringue, D. Dormont, J.P. Desleys (1999): Pathogenesis of the oral route of infection of mice with scrapie and bovine spongiform encephalopathy agents. J. Gen. Virol. 80, 3035-3042.
15. Nave, K.-A., C. Lai, F.E. Bloom, R.J. Millner (1987): Splice site selection in the proteolipid protein (PLP) gene transcript and primary structure of the DM-20 protein of central nervous system myelin. Proc. Natl. Acad. Sci. USA 84, 5665-5669.
16. Pamh-Dinh, D., M.C. Birling, G. Roussel, A. Dautigny, J.L. Nussbaum (1991 ): Proteolipid DM-20 predominates over PLP in peripheral nervous system. Neuroreport 2, 89-92.
17. Prusiner, S.B. (1982): Novel proteinaceous infectious particles cause scrapie. Science 216, 136-144.
18. Regulation EC No. 999/2001: Regulation (EC) No. 999/2001 of the European Parliament and of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. Off. J. Eur. Comm. L 147, 1-40.
19. Sandmeier, B., C. Villmann, T. Düthorn, M. Gareis, C.M. Becker, M. Pischetsrieder (2006): Myelin proteolipid protein as a new target for the detection of central nervous tissues in food. Lebensmittelchemie 60, 19.
20. Sandmeier, B., C. Villmann, R. Bäuerlein, T. Düthorn, M. Gareis, C.M. Becker, M. Pischetsrieder (2007): Detection of central nervous system tissue in meat and meat products with a newly developed immunoassay selective for myelin proteolipid protein. Food Chem., available online at: doi:10.1016/j.foodchem.2007.01.071.
21. Schmidt, G.R., K.L. Hossner, R.S. Yemm, D.H. Gould, J.P. O'Callaghan (1999): An enzyme-linked immunosorbent assay for glial fibrillary acidic protein as an indicator of the presence of brain or spinal cord in meat. J. Food Prot. 62, 394-397.
22. Villmann, C., B. Sandmeier, M. Pischetsrieder, C.M. Becker (2007): Myelin proteoplipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. J. Agric. Food Chem., available online at: doi: 10.1021/jf0707278.
23. Will, R.G., J.W. Ironside, M. Zeidler, S.N. Cousens, K. Estibeiro, A. Alperovitch, S. Poser, M. Pocchiari, A. Hofman (1996): A new variant of Creutzfeldt-Jacob disease in UK. Lancet 347, 921-925.
24. WHO (2007a): Bovine spongiform encephalopathy. http:/www.who. int/zoonoses/diseases/bse/en.
25. WHO (2007b): Variant Creutzfeldt Jakob disease. http:/www.who. int/zoonoses/diseases/variantcjd/en.