Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex

Molecular Biology of the Cell

Volumn 18, Issue 12, 2007, Pages 4992-5003

  Pavicic Kaltenbrunner, Visnja ^a   Mishima, Masanori ^b   Glotzer, Michael ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; GAP JUNCTION PROTEIN; KINESIN; KINESIN 6; RAC PROTEIN; RHO FACTOR; UNCLASSIFIED DRUG;

ANAPHASE; ARTICLE; CONTROLLED STUDY; CYTOKINESIS; GENE ISOLATION; GENETIC ANALYSIS; NONHUMAN; PLASTICITY; PRIORITY JOURNAL; PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CELL CYCLE PROTEINS; CONSERVED SEQUENCE; DIMERIZATION; GENE EXPRESSION REGULATION; GTPASE-ACTIVATING PROTEINS; HUMANS; KINESIN; MALE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHOPROTEINS; PROTEIN BINDING; SEQUENCE ALIGNMENT;

METAZOA;

EID: 37049038634     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E07-05-0468     Document Type: Article

References (34)

1. Anderson, P. (1995). Mutagenesis. Methods Cell Biol. 48, 31-58.
2. Berger, B., Wilson, D. B., Wolf, E., Tonchev, T., Milla, M., and Kim, P. S. (1995). Predicting coiled coils by use of pairwise residue correlations. Proc. Natl. Acad. Sci. USA 92, 8259-8263.
3. Bringmann, H., and Hyman, A. A. (2005). A cytokinesis furrow is positioned by two consecutive signals. Nature 436, 731-734.
4. Dechant, R., and Glotzer, M. (2003). Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation. Dev. Cell 4, 333-344.
5. Desai, A., and Walczak, C. E. (2001). Assays for microtubule- destabilizing kinesins. Methods Mo;. Biol. 164, 109-121.
6. Falke, J. J., Dernburg, A. F., Sternberg, D. A., Zalkin, N., Milligan, D. L., and Koshland, D.E.J. (1988). Structure of a bacterial sensory receptor. A site-directed sulfhydryl study. J. Biol. Chem. 263, 14850-14858.
7. Glotzer, M. (2005). The molecular requirements for cytokinesis. Science 307, 1735-1739.
8. Gromley, A., Yeaman, C., Rosa, J., Redick, S., Chen, C. T., Mirabelle, S., Guha, M., Sillibourne, J., and Doxsey, S. J. (2005). Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle- mediated abscission. Cell 123, 75-87.
9. Jantsch-Plunger, V., Gönczy, P., Romano, A., Schnabel, H., Hamill, D., Schnabel, R., Hyman, A. A., and Glotzer, M. (2000). CYK-4, A Rho family GTPase activating protein (GAP) required for central spindle formation and cytokinesis. J. Cell Biol. 149, 1391-1404.
10. Kaitna, S., Mendoza, M., Jantsch-Plunger, V., and Glotzer, M. (2000). Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis. Curr. Biol. 10, 1172-1181.
11. Kamijo, K., Ohara, N., Abe, M., Uchimura, T., Hosoya, H., Lee, J. S., and Miki, T. (2006). Dissecting the role of Rho-mediated signaling in contractile ring formation. Mol. Biol. Cell 17, 43-55.
12. Kurasawa, Y., Earnshaw, W. C., Mochizuki, Y., Dohmae, N., and Todokoro, K. (2004). Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 23, 3237-3248.
13. Lupas, A., Van Dyke, M., and Stock, J. (1991). Predicting coiled coils from protein sequences. Science 252, 1162-1164.
14. Mello, C., and Fire, A. (1995). DNA transformation. Methods Cell Biol. 48, 451-482.
15. Mishima, M., Kaitna, S., and Glotzer, M. (2002). Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell 2, 41-54.
16. Mishima, M., Pavicic, V., Gruneberg, U., Nigg, E. A., and Glotzer, M. (2004). Cell cycle regulation of central spindle assembly. Nature 430, 908-913.
17. Mollinari, C., Kleman, J. P., Jiang, W., Schoehn, G., Hunter, T., and Margolis, R. L. (2002). PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 157, 1175-1186.
18. Mollinari, C., Kleman, J. P., Saoudi, Y., Jablonski, S. A., Perard, J., Yen, T. J., and Margolis, R. L. (2005). Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not. Mol. Biol. Cell 16, 1043-1055.
19. Nishimura, Y., and Yonemura, S. (2006). Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. J. Cell Sci. 119, 104-114.
20. O'Shea, E. K., Klemm, J. D., Kim, P. S., and Alber, T. (1991). X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil. Science 254, 539-544.
21. Oakley, M. G., and Kim, P. S. (1998). A buried polar interaction can direct the relative orientation of helices in a coiled coil. Biochemistry 37, 12603-12610.
22. Pereira, G., and Schiebel, E. (2003). Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science 302, 2120-2124.
23. Powers, J., Bossinger, O., Rose, D., Strome, S., and Saxton, W. (1998). A nematode kinesin required for cleavage furrow advancement. Curr. Biol. 8, 1133-1136.
24. Raich, W. B., Moran, A. N., Rothman, J. H., and Hardin, J. (1998). Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4. Mol. Biol. Cell 9, 2037-2049.
25. Sessa, F., Mapelli, M., Ciferri, C., Tarricone, C., Areces, L. B., Schneider, T. R., Stukenberg, P. T., and Musacchio, A. (2005). Mechanism of Aurora B activation by INCENP and inhibition by hesperadin. Mol. Cell 18, 379-391.
26. Severson, A. F., Hamill, D. R., Carter, J. C., Schumacher, J., and Bowerman, B. (2000). The aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis. Curr. Biol. 10, 1162-1171.
27. Somers, W. G., and Saint, R. (2003). A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev. Cell. 4, 29-39.
28. Toure, A., Dorseuil, O., Morin, L., Timmons, P., Jegou, B., Reibel, L., and Gacon, G. (1998). MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells. J. Biol. Chem. 273, 6019-6023.
29. Verbrugghe, K. J., and White, J. G. (2004). SPD-1 is required for the formation of the spindle midzone but is not essential for the completion of cytokinesis in C. elegans embryos. Curr. Biol. 14, 1755-1760.
30. Verni, F., Somma, M. P., Gunsalus, K. C., Bonaccorsi, S., Belloni, G., Goldberg, M. L., and Gatti, M. (2004). Feo, the Drosophila homolog of PRC1, is required for central-spindle formation and cytokinesis. Curr. Biol. 14, 1569-1575.
31. Werner, M., Munro, E., and Glotzer, M. (2007). Astral signals spatially bias cortical Myosin recruitment to break symmetry and promote cytokinesis. Curr. Biol. 17, 1286-1297.
32. Wheatley, S. P., and Wang, Y. (1996). Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells. J. Cell Biol. 135, 981-989.
33. Yuce, O., Piekny, A., and Glotzer, M. (2005). An ECT2-centralspindlin complex regulates the localization and function of RhoA. J. Cell Biol. 170, 571-582.
34. Zhao, W. M., and Fang, G. (2005). MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis. Proc. Natl. Acad. Sci. USA 102, 13158-13163.