Functional assembly of the Na+/H+ antiporter of Helicobacter pylori from partial fragments in vivo

Biochemistry

Volumn 46, Issue 49, 2007, Pages 14272-14283

  Karasawa, Akira ^a   Mitsui, Keiji ^a   Matsushita, Masafumi ^a   Kanazawa, Hiroshi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIPORTER ACTIVITY; COMPLEMENTARY FRAGMENT PAIRS; PARTIAL FRAGMENTS; PLASMIDS; TRANSMEMBRANE DOMAINS (TM);

CELL MEMBRANES; ESCHERICHIA COLI; SODIUM COMPOUNDS;

BACTERIA;

ANTIPORTER; HYDROGEN ANTIPORTER; SODIUM ANTIPORTER; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE SEQUENCE; HELICOBACTER PYLORI; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; HELICOBACTER PYLORI; MODELS, MOLECULAR; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, SECONDARY; SODIUM-HYDROGEN ANTIPORTER; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI; HELICOBACTER PYLORI;

EID: 37049016054     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi701627e     Document Type: Article

References (45)

1. Padan, E., and Schuldiner, S. (1992) Alkali Cation Transport System in Procaryotes (Bakker, E., Ed.) pp 3-24, CRC Press, Inc., Boca Raton, FL.
2. + in cells, Biochim. Biophys. Acta 1185, 129-151.
3. Schuldiner, S., and Padan, E. (1992) Alkali Cation Transport Systems in Procaryotes (Bakker, E., Ed.) pp 25-51, CRC Press, Inc., Boca Raton, FL.
4. Padan, E. (1998) Microbiology and Biochemistry of Hyper Saline Environments (Oren, A., Ed.) pp 163-175, CRC Press, Inc., Boca Raton, FL.
5. + antiporter activity in Escherichia coli, J. Biol. Chem. 263, 10408-10414.
6. + antiporter in Escherichia coli, J. Biol. Chem. 267, 11064-11068.
7. + antiporter- deficient strains by the overexpressed gene, J. Biol. Chem. 268, 11296-11303.
8. + antiporter coded by nhaA (ant) from Escherichia coli, J. Biol. Chem. 266, 11289-11294.
9. + antiporter from Escherichia coli, J. Biol. Chem. 269, 26274-26279.
10. + antiporter from Escherichia coli, J. Biol. Chem. 271, 32288-32292.
11. + antiporter (NhaA) from Escherichia coli, FEBS Lett. 363, 264-268.
12. + antiporter (NhaA) protein from Helicobacter pylori required for ion transport and pH sensing, J. Biol. Chem. 278, 21467-21473.
13. + antiporters of Helicobacter pylori in antiporter-deficient Escherichia coli mutants, FEBS Lett. 443, 11-16.
14. + antiporter NhaA faces a water-filled channel required for ion transport, J. Biol. Chem. 279, 40567-40575.
15. + antiporter of Helicobacter pylori: Topology and function of the residues, including two consecutive essential aspartate residues, Biochemistry 45, 14834-14842.
16. + antiporter and insights into mechanism of action and regulation by Ph, Nature 435, 1197-1201.
17. + antiporter of Escherichia coli, J. Biol. Chem. 279, 23104-23113.
18. Popot, J. L., and Engelman, D. M. (1990) Membrane protein folding and oligomerization: the two-stage model, Biochemistry 29, 4031-4037.
19. Engelman, D. M., Chen, Y., Chin, C. N., Curran, A. R., Dixon, A. M., Dupuy, A. D., Lee, A. S., Lehnen, U., Matthews, E. E., Reshetnyak, Y. K., Senes, A., and Popot, J. L. (2003) Membrane protein folding: beyond the two stage model, FEBS Lett. 555, 122-125.
20. Groves, J. D., and Tanner, M. J. A., (1995) Co-expressed complementary fragments of the human red cell anion exchanger (band 3, AE1) generate stilbene disulfonate-sensitive anion transport, J. Biol. Chem. 270, 9097-9105.
21. Wang, J. D., Groves, J. D., Mawby, W. J., and Tanner, M. J. A. (1997) Complementation studies with co-expressed fragments of the human red cell anion transporter (band 3; AE1). The role of some exofacial loops in anion transport, J. Biol. Chem. 272, 10631-10638.
22. Groves, J. D., Wang, L., and Tanner, M. J. A. (1998) Functional reassembly of the anion transport domain of human red cell band 3 (AE1) from multiple and non-complementary fragments, FEBS Lett. 433, 223-227.
23. Martin, N. P., Leavitt, L. M., Sommers, C. M., and Dumont, M. E. (1999) Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments, Biochemistry 38, 682-695.
24. Bibi, E., and Kaback, H. R. (1990) In vivo expression of the lacY gene in two segments leads to functional lac permease, Proc. Natl. Acad. Sci. U.S.A. 87, 4325-4329.
25. Yamaguchi, A., Someya, Y., and Sawai, T. (1993) The in vivo assembly and function of the N- and C-terminal halves of the Tn10-encoded TetA protein in Escherichia coli, FEBS Lett. 324, 131-135.
26. + antiporter gene of Escherichia coli, J. Bacteriol. 173, 6553-6557.
27. Messing, J., and Vieira, J. (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments, Gene (Amsterdam) 19, 269-276.
28. Kanazawa, H., Miki, T., Tamura, F., Yura, T., and Futai, M. (1979) Specialized transducing phage lambda carrying the genes for coupling factor of oxidative phosphorylation of Escherichia coli: increased synthesis of coupling factor on induction of prophage lambda asn, Proc. Natl. Acad. Sci. U.S.A. 76, 1126-1130.
29. + antiporter from Helicobacter pylori by fluorescence resonance energy transfer, J. Biol. Chem. 280, 41900-41911.
30. Inoue, H., Tsuboi, Y., and Kanazawa, H. (2001) Chimeric Na(+)/H(+) antiporters constructed from NhaA of Helicobacter pylori and Escherichia coli: implications for domains of NhaA for pH sensing, J. Biochem. 129, 569-576.
31. + antiporter of Vibrio parahaemolyticus, Biochem. Biophys. Res. Commun. 222, 774-779.
32. Maniatis, T., Fritsch, F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
33. Ito, K., and Akiyama, Y. (2005) Cellular functions, mechanism of action, and regulation of FtsH protease, Annu. Rev. Microbiol. 59, 211-231.
34. 32 by Escherichia coli AAA protease FtsH, J. Struct. Biol. 146. 148-154.
35. Sakaguchi, M. (2002) Autonomous and heteronomous positioning of transmembrane segments in multispanning membrane protein, Biochem. Biophys. Res. Commun. 296, 1-4.
36. Schmidt-Rose, T., and Jentsch, T. J. (1997) Reconstitution of functional voltage-gated chloride channels from complementary fragments of CLC-1, J. Biol. Chem. 272, 20515-20521.
37. Chan, K. W., Csanady, L., Seto-Young, D., Nairn, A. C., and Gadsby, D. C. (2000) Severed molecules functionally define the boundaries of the cystic fibrosis transmembrane conductance regulator's NH(2)-terminal nucleotide binding domain, J. Gen. Physiol. 116, 163-180.
38. Liao, M. J., Huang, K. S., and Khorana, H. G. (1984) Regeneration of native bacteriorhodopsin structure from fragments, J. Biol. Chem. 259, 4200-4204.
39. Marti, T. (1998) Refolding of bacteriorhodopsin from expressed polypeptide fragments, J. Biol. Chem. 273, 9312-9322.
40. Ridge, K. D., Lee, S. J., and Yao, L. L. (1995) In vivo assembly of rhodopsin from expressed polypeptide fragments, Proc. Natl. Acad. Sci. U.S.A. 92, 3204-3208.
41. Ridge, K. D., Lee, S. S., and Abdulaev, N. G. (1996) Examining rhodopsin folding and assembly through expression of polypeptide fragments, J. Biol. Chem. 271, 7860-7867.
42. Zen, K. H., McKenna, E., Bibi, E., Hardy, D., and Kaback, H. R. (1994) Expression of lactose permease in contiguous fragments as a probe for membrane-spanning domains, Biochemisty. 33, 8198-8206.
43. +-phosphate cotransport system (NaPi-II) with a cleaved protein backbone: implications on function and membrane insertion, J. Physiol. 508, 341-350.
44. Wilkinson, B. M., Esnault, Y., Craven, R. A., Skiba, F., Fieschi, J., Kepes, F. and Stirling, C. J. (1997) Molecular architecture of the ER translocase probed by chemical crosslinking of Sss1p to complementary fragments of Sec61p, EMBO J. 16, 4549-4559.
45. Ridge, K. D., Ngo, T., Lee, S. S. J., and Abdulaev, N. G. (1999) Folding and assembly in rhodopsin. Effect of mutations in the sixth transmembrane helix on the conformation of the third cytoplasmic loop, J. Biol. Chem. 274, 21437-21442.