Quantitative detection of Citrus tristeza virus in citrus and aphids by real-time reverse transcription-PCR (TaqMan®)

Journal of Virological Methods

Volumn 147, Issue 1, 2008, Pages 43-53

  Saponari, Maria ^a   Manjunath, Keremane ^a   Yokomi, Raymond K ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Closterovirus; Disease diagnosis; Real time PCR; RNA extraction; Vectors

Indexed keywords

MESSENGER RNA;

ANALYTIC METHOD; APHID; ARTICLE; CITRUS FRUIT; CITRUS TRISTEZAVIRUS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT VIRUS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS; VIRUS DETECTION; VIRUS STRAIN;

ANIMALS; APHIDS; CITRUS; CLOSTEROVIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; PLANT DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

APHIDIDAE; APHIS GOSSYPII; CITRUS; CITRUS TRISTEZA VIRUS; CLOSTEROVIRUS;

EID: 37049015789     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.07.026     Document Type: Article

References (48)

1. Barbarossa L., and Savino V. Sensitive and specific digoxigenin-labelled RNA probes for routine detection of Citrus tristeza virus by dot-blot hybridization. J. Phytopathol. 154 (2006) 329-335
2. Bar-Joseph M., Garnsey S.M., Gonsalves D., Moscovitz M., Purcifull D.E., Clark M.F., and Loebenstein G. The use of enzyme-linked immunosorbent assay for detection of Citrus tristeza virus. Phytopathology 69 (1979) 190-194
3. Boonham N., Smith P., Walsh K., Tame J., Morris J., Spence N., Bennison J., and Barker I. The detection of tomato spotted wilt virus (TSWV) in individual thrips using real-time fluorescent RT-PCR (TaqMan). J. Virol. Methods 101 (2002) 37-48
4. Cambra M., Garnsey S.M., Permar T.A., Henderson C.T., Gumpf D., and Vela C. Detection of Citrus tristeza virus (CTV) with a mixture of monoclonal antibodies. Phytopathology 80 (1990) 103
5. Cambra M., Camarasa E., Gorris M.T., Garnsey S.M., and Carbonell E. Comparison of different immunosorbent assays for Citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies. In: Brlansky R.H., Lee R.H., and Timmer L.W. (Eds). Proceedings of the 11th Conference of International Organization of Citrus Virologists (1991), IOCV, Riverside, CA 38-45
6. Cambra M., Gorris M.T., Román M.P., Terrada E., Garnsey S.M., Camarasa E., Olmos A., and Colomer M. Routine detection of Citrus tristeza virus by direct immunoprinting-ELISA methods using specific monoclonal and recombinant antibodies. In: da Graça J.V., Lee R.F., and Yokomi R.K. (Eds). Proceedings of the 14th Conference of International Organization of Citrus Virologists (2000), IOCV, Riverside, CA 34-41
7. Cambra M., Olmos A., Gorris M.T., Marroquín C., Esteban O., Garnsey S.M., Llauger R., Batista L., Peña I., and Hermoso de Mendoza A. Detection of Citrus tristeza virus by print capture and squash capture-PCR in plant tissue and single aphids. In: da Graça J.V., Lee R.F., and Yokomi R.K. (Eds). Proceedings of the 14th Conference of International Organization of Citrus Virologists (2000), IOCV, Riverside, CA 42-49
8. Cambra M., Gorris M.T., Olmos A., Martínez M.C., Román M.P., Bertolini E., López A., and Carbonell E.A. European diagnostic protocol (DIAGPRO) for Citrus tristeza virus in adult trees. In: Duran-Vila N., Milne R.G., and da Graça (Eds). Proceedings of the 15th Conference of International Organization of Citrus Virologists (2002), IOCV, Riverside, CA 69-77
9. Cevik B., Pappu S.S., Pappu H.R., Benscher D., Irey M., Lee R.F., and Niblett C.L. Application of bi-directional PCR to Citrus tristeza virus: detection and strain differentiation. In: da Graça J.V., Moreno P., and Yokomi R.K. (Eds). Proceedings of the 13th Conference of International Organization of Citrus Virologists (1996), IOCV, Riverside, CA 17-24
10. Fabre F., Kervarrec C., Mieuzet L., Riault G., Vialatte A., and Jacquot E. Improvement of barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real-time RT-PCR. J. Virol. Methods 110 (2003) 51-60
11. Foissac X., Svanella-Dumas L., Dulucq M.J., Candresse T., and Gentit P. Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested-PCR using degenerated and inosine containing primers. Acta Horticolturae 550 (2001) 37-44
12. Garnsey S.M., Permar T.A., Cambra M., and Henderson C.T. Direct tissue blot immunoassay (DTBIA) for detection of Citrus tristeza virus (CTV). In: Moreno P., da Graça J.V., and Timmer L.W. (Eds). Proceedings of the 12th Conference of International Organization of Citrus Virologists (1993), IOCV, Riverside, CA 39-50
13. Garnsey S.M., Gottwald T.R., and Borbón J.C. Rapid dissemination of mild isolates of Citrus tristeza virus following introduction of Toxoptera citricida in the Dominican Republic. In: da Graça J.V., Moreno P., and Yokomi R.K. (Eds). Proceedings of the 13th Conference of International Organization of Citrus Virologists (1996), IOCV, Riverside, CA 92-103
14. Garnsey S.M., Civerolo E., Gumpf D.J., Paul C., Hilf M.E., Lee R.F., Brlansky R.H., Yokomi R.K., and Hartung J.S. Biological characterization of an international collection of Citrus tristeza virus (CTV) isolates. In: Hilf M.E., Duran-Vila N., and Rocha-Peña M.A. (Eds). Proceedings of the 16th Conference of International Organization of Citrus Virologists (2005), IOCV, Riverside, CA 75-93
15. Halbert S.E., Genc H., Cevik B., Brown L.G., Rosales I.M., Manjunath K., Pomerinke M., Davison D.A., Lee R.F., and Niblett C.L. Distribution and characterization of Citrus tristeza virus in south Florida following establishment of Toxoptera citricida. Plant Dis. 88 (2004) 935-941
16. Hilf M.E., and Garnsey S.M. Characterization and classification of Citrus tristeza virus isolate by amplification of multiple molecular markers. In: da Graça J.V., Lee R.F., and Yokomi R.K. (Eds). Proceedings of the 14th Conference of International Organization of Citrus Virologists (2000), IOCV, Riverside, CA 18-27
17. Hilf M.E., Mavrodieva V.A., and Garnsey S.M. Genetic marker analysis of a global collection of isolates of Citrus tristeza virus: characterization and distribution of CTV genotypes and association with symptoms. Phytopathology 95 (2005) 909-917
18. Huang Z., Rundell A.P., Guan X., and Powell A.C. Detection and isolate differentiation of Citrus tristeza virus in infected field trees based on reverse transcription-polymerase chain reaction. Plant Dis. 88 (2004) 625-629
19. Hung T.H., Wu M.L., and Su H.J. A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of Citrus tristeza virus. J. Phytopathol. 148 (2000) 469-475
20. Karasev A.V., Boyko V.P., Gowda S., Nikolaeva O., Hilf M.E., Koonin E.V., Niblett C.L., Cline K., Gumpf D., Lee R.F., Garnsey S.M., Levandowski D.J., and Dawson W.O. Complete sequence of the Citrus tristeza virus RNA genome. Virology 208 (1995) 511-520
21. Lee R.F., and Bar-Joseph M. Tristeza. In: Timmer L.W., Garnsey S.M., and Graham J.H. (Eds). Compendium of Citrus Diseases (2000), APS Press, St. Paul, MN 61-63
22. Mackay I., Arden K., and Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 30 (2002) 1292-1305
23. Marbot S., Salmon M., Vandrame M., Huwaert A., Kummert J., Dutrecq O., and Lepoivre P. Development of real-time RT-PCR assay for detection of prunus necrotic virus in fruit trees. Plant Dis. 87 (2003) 1344-1348
24. Marroquin C., Olmos A., Gorris M.T., Bertolini E., Martinez M.C., Carbonell E.A., Hermoso de Mendoza A., and Cambra M. Estimation of the number of aphids carrying Citrus tristeza virus that visit adult citrus trees. Virus Res. 100 (2004) 101-108
25. Martelli G.P., Agranovsky A.A., Bar-Joseph M., Boscia D., Candresse T., Coutts R.H.A., Dolja V.V., Falk B.W., Gonsalves D., Hu J., Jelkmann, Karasev A.V., Minafra A., Namba S., Vetten H.J., Wisler C.G., and Yoshikawa N. Virus taxonomy. In: Fauquet C.M., Mayo M.A., Maniloff J., Desselberger U., and Ball L.A. (Eds). Eighth Report of the International Committee on Taxonomy of Viruses (2005), Elsevier Academic Press, San Diego, CA
26. Mathews D.M., Riley K., and Dodds J.A. Comparison of detection methods for Citrus tristeza virus in field trees during months of nonoptimal titer. Plant Dis. 81 (1997) 525-529
27. Mawassi M., Mietkiewska E., Gofman R., Yang G., and Bar-Joseph M. Unusual sequences relationships between two isolates of Citrus tristeza virus. J. Gen. Virol. 77 (1996) 2359-2364
28. Menzel W., Jelkmann W., and Maiss E. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J. Virol. Methods 99 1/2 (2002) 81-92
29. Metha P., Brlansky R.H., Gowda S., and Yokomi R.K. Reverse-transcription polymerase chain reaction detection of Citrus tristeza virus in aphids. Plant Dis. 81 (1997) 1066-1069
30. Niblett C.L., Genc H., Cevik B., Halbert S., Brown L., Nolasco G., Bonacalza B., Manjunath K.L., Febres V.J., Pappu H.R., and Lee R.F. Progress on strain differentiation of Citrus tristeza virus and its application to the epidemiology of citrus tristeza disease. Virus Res. 71 (2000) 97-106
31. Nikolaeva O.V., Karasev A.V., Powell A.C., Gumpf D.J., Garnsey S.M., and Lee R.F. Mapping of epitopes for Citrus tristeza virus-specific monoclonal antibodies using bacterially expressed coat protein fragment. Phytopathology 86 (1996) 974-979
32. Olmos A., Bertolini E., Gil M., and Cambra M. Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. J. Virol. Methods 128 (2005) 151-155
33. Osman F., and Rowhani A. Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan). J. Virol. Methods 133 (2006) 130-136
34. ®) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9. J. Virol. Methods 141 (2007) 22-29
35. Polek M., Gumpf D.J., Wallen C.M., and Riley K.M. Biological characterization of naturally occurring Citrus tristeza virus strains in California. In: Hilf M.E., Duran-Vila N., and Rocha-Peña M.A. (Eds). Proceedings of the 16th Conference of International Organization of Citrus Virologists (2005), IOCV, Riverside, CA 68-74
36. Rocha-Pena M.A., and Lee R.F. Serological technique for detection of Citrus tristeza virus. J. Virol. Methods 34 (1991) 311-331
37. Roistacher C.N. Graft-Transmissible Diseases of Citrus: Handbook for Detection and Diagnosis (1991), IOCV and FAO, Rome p. 286
38. Roy A., Fayad A., Barthe G., and Brlansky R.H. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. J. Virol. Methods 129 (2005) 47-55
39. Schneider W.L., Sherman D.J., Stone A.L., Damsteegt V.D., and Frederick R.D. Specific detection and quantification of plum pox virus by real-time fluorescent reverse transcription-PCR. J. Virol. Methods 120 (2004) 97-105
40. Sieburth P.J., Nolan K.G., Hilf M.E., Lee R.F., Moreno P., and Garnsey S.M. Discrimination of stem-pitting from other isolates of Citrus tristeza virus. In: Hilf M.E., Duran-Vila N., and Rocha-Peña M.A. (Eds). Proceedings of the 16th Conference of International Organization of Citrus Virologists (2005), IOCV, Riverside, CA 1-10
41. Singh R.P., Dilworth A.D., Singh M., and McLaren D.L. Evaluation of a simple membrane-based nucleic acid preparation protocol for RT-PCR detection of potato viruses from aphid and plant tissues. J. Virol. Methods 121 (2004) 163-170
42. Terrada E., Kerschbaumer R.J., Giunta G., Galeffi P., Himmler G., and Cambra M. Fully "recombinant enzyme-linked immunosorbent assay" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus. Phytopathology 90 (2000) 1337-1344
43. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24 (1997) 4876-4882
44. Varga A., and James D. Detection and differentiation of plum pox virus using real-time multiplex PCR with SYBR green and melting curve analysis: a rapid method for strain typing. J. Virol. Methods 123 (2005) 213-220
45. Varga A., and James D. Real-time RT-PCR and SYBR green I melting curve analysis for the identification of plum pox virus strains C, EA, and W: effect of amplicon size, melt rate, and dye translocation. J. Virol. Methods 132 (2006) 146-153
46. Wallace J.M., and Drake R.J. Recent developments in studies of quick decline and related diseases. Phytopathology 41 (1951) 785-793
47. Wang, C., Ni, H., Wang, G.P., Zhang, J.K., Hui, L., 2007. Molecular analysis of a Chinese Citrus tristeza virus isolate with differential serological reactivity. J. Plant Pathol. 89, in press.
48. Yokomi R.K., and Deborde R.L. Incidence, transmissibility, and genotype of Citrus tristeza virus (CTV) isolates from a CTV eradicative and a non-eradicative district in central California. Plant Dis. 89 8 (2005) 859-866