An improved method of gene synthesis based on DNA works software and overlap extension PCR

Molecular Biotechnology

Volumn 37, Issue 3, 2007, Pages 195-200

  Dong, Bingxue ^a   Mao, Runqian ^b   Li, Baojian ^a   Liu, Qiuyun ^a   Xu, Peilin ^a   Li, Gang ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b Guangdong Academy of Sciences   (China)

Author keywords

DNA Works; Gene synthesis; OE PCR

Indexed keywords

AUTOMATION; BIOASSAY; COMPUTER SOFTWARE; DNA; DNA SEQUENCES; MUTAGENESIS; OLIGONUCLEOTIDES;

DNA WORKS; GENE SYNTHESIS; PHANEROCHAETE CHRYSOSPORIUM;

GENE ENCODING;

CYCLIN DEPENDENT KINASE INHIBITOR 1; DNA FRAGMENT; LACCASE; MANGANESE PEROXIDASE;

ARTICLE; AUTOMATION; COMPUTER PROGRAM; CONTROLLED STUDY; DNA SEQUENCE; GENE SYNTHESIS; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

CLONING, MOLECULAR; DATABASES, NUCLEIC ACID; GENES; MODELS, BIOLOGICAL; PEROXIDASES; PICHIA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SOFTWARE;

COPRINOPSIS CINEREA; PHANEROCHAETE CHRYSOSPORIUM; TRAMETES VERSICOLOR;

EID: 36949020332     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-0039-8     Document Type: Article

References (16)

1. Van Hest, J. C. M., & Tirrell, D. A. (2001). Protein-based materials, toward a new level of structural control. Chemical Communications, 19, 1897-1904.
2. Gustafsson, C., Govindarajan, S., & Minshull, J. (2004). Codon bias and heterologous protein expression. Trends in Biotechnology, 22, 346-353.
3. Yang, Z. Y., Kong, W. P., & Huang, Y., et al. (2004). A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice. Nature, 428, 561-564.
4. Scarpulla, R. C., Narang, S., & Wu, R. (1982). Use of a new retrieving adaptor in the cloning of a synthetic human insulin A-chain gene. Anaytical Biochemistry, 121, 356-365.
5. Mandecki, W., & Bolling, T. J. (1988). FokI method of gene synthesis. Gene, 68, 101-107.
6. Dillon, P. J., & Rosen, C. A. (1990). A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques, 9, 298-300.
7. Chen, G. Q., Choi, I., & Ramachandran, B., et al. (1994). Total gene synthesis: novel single step and convergent strategies applied to the costruction of a 779 base pair bacteriorhodopsin gene. Journal of the American Chemical Society, 11, 8799-8800.
8. Hayashi, N., Welschof, M., & Zewe, M., et al. (1994). Simutaneous mutagenesis of antibody CDR regions by overlap extension and PCR. Biotechniques, 17, 310-314.
9. Stemmer, W. P., Crameri, A., & Ha, K. D., et al. (1995). Single-step assembly of a gene and entire plasmid from large number of oligodeoxyribonucleotides. Gene, 164, 49-53.
10. Lin, Y., Cheng, X., & Clark, T. G. (2002). The use of synthetic genes for the expression of ciliate proteins in heterologous systems. Gene, 288, 85-94.
11. Gao, X., Yo, P., & Keith, A., et al. (2003). Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Research, 31, e134.
12. Young, L., & Dong, Q. H. (2004). Two-step total gene synthesis method. Nucleic Acids Research, 32, e59.
13. Hoover, D. M., & Lubkowski, J. (2002). DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucleic Acids Research, 30, e43.
14. An, Y. F., Ji, J. F., & Wu, W. F., et al. (2005). A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR. Applied Microbiology and Biotechnology, 68, 774-778.
15. Smith, H. O., Hutchison, C. A., & Pfannkoch, C., et al. (2003). Generating a synthetic genome by whole genome assembly: phi X174 bacteriophage from synthetic oligonucleotides. Proceedings of the National Academy of Sciences of the United States of America, 100, 15440-15445.
16. Xiong, A. S., Yao, Q. H., & Peng, R. H., et al. (2004). A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences. Nucleic Acids Research, 32, e98.