Morphological and physiological properties of serotonergic neurons in dissociated cultures from the postnatal rat dorsal raphe nucleus

Journal of Neuroscience Methods

Volumn 167, Issue 2, 2008, Pages 258-267

  Yasufuku Takano, Junko ^a   Nakajima, Shigehiro ^a   Nakajima, Yasuko ^a  

^a UNIVERSITY OF ILLINOIS   (United States)

Author keywords

Bombesin; Gastrin releasing peptide; Inward rectifier K currents; Phenylephrine; Postnatal rat dorsal raphe nucleus; Serotonergic neuron culture; Serotonin immunocytochemistry; Whole cell patch clamp

Indexed keywords

2 DIPROPYLAMINO 8 HYDROXYTETRALIN; BOMBESIN; GASTRIN RELEASING PEPTIDE; PHENYLEPHRINE;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; BRAIN SLICE; CONTROLLED STUDY; CULTURE MEDIUM; DEPOLARIZATION; DORSAL RAPHE NUCLEUS; ELECTRIC POTENTIAL; GLIA CELL; IMMUNOCYTOCHEMISTRY; NERVE CELL CULTURE; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SEROTONINERGIC NERVE CELL; WHOLE CELL;

8-HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN; ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, RADIATION; ELECTRIC STIMULATION; MEMBRANE POTENTIALS; NEURONS; NEUROTRANSMITTER AGENTS; PATCH-CLAMP TECHNIQUES; RAPHE NUCLEI; RATS; SEROTONIN; SEROTONIN AGONISTS; TIME FACTORS;

EID: 36849058170     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.08.018     Document Type: Article

References (59)

1. Aghajanian G.K. Modulation of a transient outward current in serotonergic neurones by alpha 1-adrenoceptors. Nature 315 (1985) 501-503
2. Aghajanian G.K., and Lakoski J.M. Hyperpolarization of serotonergic neurons by serotonin and LSD: studies in brain slices showing increased K+ conductance. Brain Res 305 (1984) 181-185
3. Aghajanian G.K., and Wang R.Y. Physiology and pharmacology of central serotonergic neurons. In: Lipton M.A., Dimascio A., and Killam K.F. (Eds). Psychopharmacology: a generation of progress (1978), Raven Press, New York 171-183
4. Aitken A.R., and Tork I. Early development of serotonin-containing neurons and pathways as seen in wholemount preparations of the fetal rat brain. J Comp Neurol 274 (1988) 32-47
5. Altman J., and Bayer S.A. Development of the brain stem in the rat. V. Thymidine-radiographic study of the time of origin of neurons in the midbrain tegmentum. J Comp Neurol 198 (1981) 677-716
6. Azmitia E.C., and Segal M. An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat. J Comp Neurol 179 (1978) 641-667
7. Bajic D., Hoang Q.V., Nakajima S., and Nakajima Y. Dissociated histaminergic neuron cultures from the tuberomammillary nucleus of rats: culture methods and ghrelin effects. J Neurosci Methods 132 (2004) 177-184
8. Baraban J.M., and Aghajanian G.K. Suppression of firing activity of 5-HT neurons in the dorsal raphe by alpha-adrenoceptor antagonists. Neuropharmacology 19 (1980) 355-363
9. Barnes N.M., and Sharp T. A review of central 5-HT receptors and their function. Neuropharmacology 38 (1999) 1083-1152
10. Battey J., and Wada E. Two distinct receptor subtypes for mammalian bombesin-like peptides. Trends Neurosci 14 (1991) 524-528
11. Battey J.F., Way J.M., Corjay M.H., Shapira H., Kusano K., Harkins R., et al. Molecular cloning of the bombesin/gastrin-releasing peptide receptor from Swiss 3T3 cells. Proc Natl Acad Sci USA 88 (1991) 395-399
12. Brunton L.L., Lazo J.S., and Parker K.L. Goodman & Gilman's the pharmacological basis of therapeutics. 11th ed. (2006), McGraw-Hill, New York
13. Corjay M.H., Dobrzanski D.J., Way J.M., Viallet J., Shapira H., Worland P., et al. Two distinct bombesin receptor subtypes are expressed and functional in human lung carcinoma cells. J Biol Chem 266 (1991) 18771-18779
14. Dahlstroem A., and Fuxe K. Evidence for the existence of monoamine-containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurons. Acta Physiol Scand Suppl Suppl. 232 (1964) 1-55
15. Dahlstroem A., and Fuxe K. Evidence for the existence of monoamine neurons in the central nervous system. II. Experimentally induced changes in the intraneuronal amine levels of bulbospinal neuron systems. Acta Physiol Scand Suppl Suppl. 247 (1965) 1-36
16. Descarries L., Watkins K.C., Garcia S., and Beaudet A. The serotonin neurons in nucleus raphe dorsalis of adult rat: a light and electron microscope radioautographic study. J Comp Neurol 207 (1982) 239-254
17. Dudai Y., Ben-Barak J., Silman I., and Gazit H. Ontogenesis and modulation of cholinergic receptors in rat brain. In: Littauer U.Z., Dudai Y., Silman I., Teichberg V.I., and Vogel Z. (Eds). Neurotransmitters and their receptors (1980), Wiley, New York 217-239
18. Farkas R.H., Chien P.Y., Nakajima S., and Nakajima Y. Properties of a slow nonselective cation conductance modulated by neurotensin and other neurotransmitters in midbrain dopaminergic neurons. J Neurophysiol 76 (1996) 1968-1981
19. Fathi Z., Corjay M.H., Shapira H., Wada E., Benya R., Jensen R., et al. BRS-3: a novel bombesin receptor subtype selectively expressed in testis and lung carcinoma cells. J Biol Chem 268 (1993) 5979-5984
20. Feldman R.S., and Quenzer L.F. Serotonin. Fundamentals of neuropsychopharmacology (1983), Sinauer Associates, Sunderland
21. Gonzalez B.J., Leroux P., Bodenant C., Laquerriere A., Coy D.H., and Vaudry H. Ontogeny of somatostatin receptors in the rat brain: biochemical and autoradiographic study. Neuroscience 29 (1989) 629-644
22. Grigg J.J., Kozasa T., Nakajima Y., and Nakajima S. Single-channel properties of a G-protein-coupled inward rectifier potassium channel in brain neurons. J Neurophysiol 75 (1996) 318-328
23. Hagiwara S., Miyazaki S., Moody W., and Patlak J. Blocking effects of barium and hydrogen ions on the potassium current during anomalous rectification in the starfish egg. J Physiol 279 (1978) 167-185
24. Hery M., Semont A., Fache M.P., Faudon M., and Hery F. The effects of serotonin on glucocorticoid receptor binding in rat raphe nuclei and hippocampal cells in culture. J Neurochem 74 (2000) 406-413
25. Hoang Q.V., Bajic D., Yanagisawa M., Nakajima S., and Nakajima Y. Effects of orexin (hypocretin) on GIRK channels. J Neurophysiol 90 (2003) 693-702
26. Hoang Q.V., Zhao P., Nakajima S., and Nakajima Y. Orexin (hypocretin) effects on constitutively active inward rectifier K+ channels in cultured nucleus basalis neurons. J Neurophysiol 92 (2004) 3183-3191
27. Jennings C.A., Harrison D.C., Maycox P.R., Crook B., Smart D., and Hervieu G.J. The distribution of the orphan bombesin receptor subtype-3 in the rat CNS. Neuroscience 120 (2003) 309-324
28. Jin Y.H., and Akaike N. Tandospirone-induced K+ current in acutely dissociated rat dorsal raphe neurones. Br J Pharmacol 124 (1998) 897-904
29. Johnson M.D. Electrophysiological and histochemical properties of postnatal rat serotonergic neurons in dissociated cell culture. Neuroscience 63 (1994) 775-787
30. Kim K.M., Nakajima Y., and Nakajima S. G protein-coupled inward rectifier modulated by dopamine agonists in cultured substantia nigra neurons. Neuroscience 69 (1995) 1145-1158
31. Kirby L.G., Pernar L., Valentino R.J., and Beck S.G. Distinguishing characteristics of serotonin and non-serotonin-containing cells in the dorsal raphe nucleus: electrophysiological and immunohistochemical studies. Neuroscience 116 (2003) 669-683
32. Koike-Tani M., Collins J.M., Kawano T., Zhao P., Zhao Q., Kozasa T., et al. Signal transduction pathway for the substance P-induced inhibition of rat Kir3 (GIRK) channel. J Physiol 564 (2005) 489-500
33. Ladenheim E.E., Jensen R.T., Mantey S.A., and Moran T.H. Distinct distributions of two bombesin receptor subtypes in the rat central nervous system. Brain Res 593 (1992) 168-178
34. Lautenschlager M., Holtje M., von Jagow B., Veh R.W., Harms C., Bergk A., et al. Serotonin uptake and release mechanisms in developing cultures of rat embryonic raphe neurons: age- and region-specific differences. Neuroscience 99 (2000) 519-527
35. Leifer D., Lipton S.A., Barnstable C.J., and Masland R.H. Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture. Science 224 (1984) 303-306
36. Lidov H.G., and Molliver M.E. Immunohistochemical study of the development of serotonergic neurons in the rat CNS. Brain Res Bull 9 (1982) 559-604
37. Masuko S., Nakajima S., and Nakajima Y. Dissociated high-purity dopaminergic neuron cultures from the substantia nigra and the ventral tegmental area of the postnatal rat. Neuroscience 49 (1992) 347-364
38. Masuko S., Nakajima Y., Nakajima S., and Yamaguchi K. Noradrenergic neurons from the locus ceruleus in dissociated cell culture: culture methods, morphology, and electrophysiology. J Neurosci 6 (1986) 3229-3241
39. Nakajima Y., and Masuko S. A technique for culturing brain nuclei from postnatal rats. Neurosci Res 26 (1996) 195-203
40. Nakajima Y., and Masuko S. Dissociated cell cultures of microdissected brainstem and basal forebrain regions on feeder layers. In: Haynes L.W. (Ed). The neuron in tissue culture (1999), John Wiley & Sons Ltd., West Sussex 510-518
41. Nakajima Y., Nakajima S., Obata K., Carlson C.G., and Yamaguchi K. Dissociated cell culture of cholinergic neurons from nucleus basalis of Meynert and other basal forebrain nuclei. Proc Natl Acad Sci USA 82 (1985) 6325-6329
42. Nishi M., Kawata M., and Azmitia E.C. Trophic interactions between brain-derived neurotrophic factor and s100beta on cultured serotonergic neurons. Brain Res 868 (2000) 113-118
43. Penington N.J., Kelly J.S., and Fox A.P. Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat. J Physiol 469 (1993) 387-405
44. Pineyro G., and Blier P. Autoregulation of serotonin neurons: role in antidepressant drug action. Pharmacol Rev 51 (1999) 533-591
45. Pinnock R.D., Reynolds T., and Woodruff G.N. Different types of bombesin receptors on neurons in the dorsal raphe nucleus and the rostral hypothalamus in rat brain slices in vitro. Brain Res 653 (1994) 119-124
46. Rudge J.S., Eaton M.J., Mather P., Lindsay R.M., and Whittemore S.R. CNTF induces raphe neuronal precursors to switch from a serotonergic to a cholinergic phenotype in vitro. Mol Cell Neurosci 7 (1996) 204-221
47. Spindel E.R., Giladi E., Brehm P., Goodman R.H., and Segerson T.P. Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor. Mol Endocrinol 4 (1990) 1956-1963
48. Steinbusch H.W. Distribution of serotonin-immunoreactivity in the central nervous system of the rat-cell bodies and terminals. Neuroscience 6 (1981) 557-618
49. Steinbusch H.W., Nieuwenhuys R., Verhofstad A.A., and Van der Kooy D. The nucleus raphe dorsalis of the rat and its projection upon the caudatoputamen. A combined cytoarchitectonic, immunohistochemical and retrograde transport study. J Physiol (Paris) 77 (1981) 157-174
50. Steinbusch H.W., Verhofstad A.A., and Joosten H.W. Localization of serotonin in the central nervous system by immunohistochemistry: description of a specific and sensitive technique and some applications. Neuroscience 3 (1978) 811-819
51. Sternberger L.A. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger L.A. (Ed). Immunocytochemistry (1979), John Wiley, New York 104-169
52. Takano K., Yasufuku-Takano J., Kozasa T., Nakajima S., and Nakajima Y. Different G proteins mediate somatostatin-induced inward rectifier K+ currents in murine brain and endocrine cells. J Physiol 502 (1997) 559-567
53. Törk I. Raphe nuclei and serotonin containing systems. In: Paxinos G. (Ed). The rat nervous system: hindbrain and spinal cord vol. 2 (1985), Academic Press, Australia 43-78
54. Vandermaelen C.P., and Aghajanian G.K. Electrophysiological and pharmacological characterization of serotonergic dorsal raphe neurons recorded extracellularly and intracellularly in rat brain slices. Brain Res 289 (1983) 109-119
55. Wada E., Way J., Shapira H., Kusano K., Lebacq-Verheyden A.M., Coy D., et al. cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor. Neuron 6 (1991) 421-430
56. Wallace J.A., and Lauder J.M. Development of the serotonergic system in the rat embryo: an immunocytochemical study. Brain Res Bull 10 (1983) 459-479
57. Yamamoto M., Steinbusch H.W., and Jessell T.M. Differentiated properties of identified serotonin neurons in dissociated cultures of embryonic rat brain stem. J Cell Biol 91 (1981) 142-152
58. Yasufuku-Takano J., Nakajima S., and Nakajima Y. Dissociated serotonergic neuron cultures from the dorsal raphe nucleus of postnatal rats: culture methods and transmitter effects. Society for Neuroscience 34th annual meeting. Program Number 620.14 (2004)
59. Yoshimura M., Higashi H., and Nishi S. Noradrenaline mediates slow excitatory synaptic potentials in rat dorsal raphe neurons in vitro. Neurosci Lett 61 (1985) 305-310