Human conjunctival epithelial precursor cells and their progeny in 3D organotypic culture

International Journal of Developmental Biology

Volumn 51, Issue 8, 2007, Pages 739-743

  Rosellini, Alfredo ^a,b   Papini, Sandra ^a,b   Giannarini, Claudio ^c   Nardi, Marco ^a   Revoltella, Roberto P ^a,b,d  

^a UNIVERSITY OF PISA   (Italy)

^b INSTITUTE OF BIOMEDICAL TECHNOLOGIES   (Italy)

^c Hospital of Lucca   (Italy)

^d University of Pisa ^*  (Italy)

Author keywords

Epithelial basal precursor; Epithelium regeneration; Interlamellar leukocytes; Niche; Stem cell

Indexed keywords

PROTEIN P63;

ANTIBODY SPECIFICITY; ANTIGEN DETECTION; ARTICLE; CELL MIGRATION; CONJUNCTIVA; EPITHELIUM CELL; HUMAN; HUMAN TISSUE; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; ORGAN CULTURE; PHENOTYPE; PRIORITY JOURNAL; PROGENY; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; ANTIGENS, CD; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COCULTURE TECHNIQUES; CONJUNCTIVA; CULTURE MEDIA; EPITHELIAL CELLS; EPITHELIUM; GELATIN; GLYCOPROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; LEUKOCYTES; MICE; NIH 3T3 CELLS; ORGAN CULTURE TECHNIQUES; PEPTIDES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS;

EID: 36749088137     PISSN: 02146282     EISSN: None     Source Type: Journal    
DOI: 10.1387/ijdb.062198ar     Document Type: Article

References (27)

1. AZUMI N. and BATTIFORA H. (1987). The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumors. Am. J. Clin. Pathol. 88: 286-296.
2. BARA J., GAUTIER R., MOURADIAN P., DECAENS C. and DAHER N. (1991). Oncofetal mucin M1 epitope family: characterization and expression during colonic carcinogenesis. Int. J. Cancer 47: 304-310.
3. BORRE M., STAUSBOL-GRON B., NERSTROM B. and OVERGAARD J. (1998). Immunohistochemical BCL-2 and Ki-67 expression predict survival in prostate cancer patients followed expectantly. Prostate Cancer 5: 268-275.
4. BOZANIC D, BOCINA I. and SARAGA-BABIC M. (2006). Involvement of cytoskeletal proteins and growth factor receptors during development of the human eye. Anat. Embryol. 211: 367-77.
5. CHISHIMA T., YANG M., MIYAGI Y., LI L. and TAN Y. (1997). Baranov E, Shimada H, Moossa AR, Penman S, Hoffman RM. Governing step of metastasis visualized in vitro. Proc. Natl. Acad. Sci. USA 94: 11573-11576.
6. CONNON C.J., NAKAMURA T., QUANTOCK A.J. and KINOSHITA S. (2006). The persistence of transplanted amniotic membrane in corneal stroma. Am. J. Ophthalmol. 141: 190-192.
7. ITAKURA Y., SASANO F., DATE F., KATO K., SEKINE H., MORI S. and NATURA H. (1996). DNA ploidy, P53 expression and cellular proliferation in normal epithelium and squamous dysplasia of non-cancerous and cancerous human oesophagi. Anticancer. Res. 16: 201-208.
8. JOSEPH A., POWELL-RICHARDS A.O., SHANMUGANATHAN V.A. and DUA H.S. (2004). Epithelial cell characteristics of cultured human limbal explants. Br. J. Ophthalmol. 88: 393-398.
9. LAVKER R.M., WEI Z.G. and SUN T.T. (1998). Phorbol ester preferentially stimulates mouse fornical conjunctival and limbal epithelial cells to proliferate in vivo. Invest. Ophthalmol. Vis. Sci. 39: 301-307.
10. MELLER D., PIRES R, T. and TSENG S.C. (2002). Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br.J.Ophthalmol. 86: 463-471.
11. MICHELINI M., ROSELLINI A., SIMONCINI T., PAPINI S. and REVOLTELLA R.P. (2004). A three-dimensional culture of the human exocervix for studying epithelial differentiation and migrating leukocytes. Differentiation 72: 1-12.
12. O'CONNELL J.T., MUTTER G.L., CVIKO A., NUCCI M., QUADE B.J., KOZAKEWICH H.P., NEFFEN E., SUN D., YANG A., MCKEON F.D. and CRUM C.P. (2001). Identification of a basal/reserve cell immunophenotype in benign and neoplastic endometrium: a study with the p53 homologue p63. Gynecol Oncol. 80: 30-36.
13. PALADINO G., MARINO C., LA TERRA MULE S., CIVIALE C., RUSCIANO D. and ENEA V. (2004). Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva. Tissue Cell 36: 323-332.
14. PAPINI S., ROSELLINI A., CAMPANI D., DEMATTEIS A., SELLI C. and REVOLTELLA R.P. (2004). Selective growth of epithelial basal cells from human prostate in a three-dimensional organ culture. Prostate 59: 383-392.
15. PAPINI S., ROSELLINI A., NARDI M., GIANNARINI C. and REVOLTELLA R.P. (2005). Selective growth and expansion of human corneal epithelial basal stem cells in a three-dimensional-organ culture. Differentiation 73: 61-68.
16. PELLEGRINI G., GOLISANO O., PATERNA P., LAMBIASE A., BONINI S., RAMA P. and DE LUCA M. (1999). Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J. Cell. Biol. 145: 769-782.
17. PFLUGFELDER S.C., TSENG S.C., YOSHINO K., MONROY D., FELIX C. and REIS B.L. (1997). Correlation of goblet cell density and mucosal epithelial membrane mucin expression with rose bengal staining in patients with ocular irritation. Ophthalmology 104: 223-235.
18. POTTEN C.S. (1997). Stem cell. Academy Press, Harcourt Brace & Co, London UK.
19. SANTOS M.S., GOMES J.A., HOFLING-LIMA A.L., RIZZO L.V., ROMANO A.C. and BELFORT R. JR. (2005). Survival analysis of conjunctival limbal grafts and amniotic membrane transplantation in eyes with total limbal stem cell deficiency. Am. J. Ophthalmol. 140: 223-230.
20. SHATOS M.A., RIOS J.D., HORIKAWA Y., HODGES R:R:, CHANG E.L., BERNARDINO C.R., RUBIN P. A.D. and DARTT D.A., (2003) Isolation and characterization of conjunctival goblet cells. Inv.Ophthalmol.Vis.Sci. 44: 2477-2486.
21. SIGNORETTI S., MONTIRONI R., MANOLA J., ALTIMARI A., TAM C., BUBLEY G., BALK S., THOMAS G., KAPLAN I., HLATKY L., HAHNFELDT P., KANTOFF P. and LODA M. (2000). p63 is a prostate basal cell marker and is required for prostate development. Am. J. Pathol. 157: 1769-1775.
22. STASIAK P.C., PURKIS P.E., LEIGH I.M. and LANE E.B. (1989). Keratin 19: predicted amino acid sequence and broad tissue distribution suggest it evolved from keratinocyte keratins. J. Invest. Dermatol. 92: 707-716.
23. TSENG S.C., JARVINEN M.J., NELSON W.G., HUANG J.W., WOODCOCK-MITCHELL J. and SUN T.T. (1982). Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell 30:361-72.
24. VASCOTTO S.G. and GRIFFITH M. (2006). Localization of candidate stem and progenitor cell markers within the human cornea, limbus and bulbar conjunctiva in vivo and in cell culture. Anat. Rec. A. Discov. Mol. Cell. Evol. Biol. 288: 921-931.
25. WARNKE R.A., GATTER K.C., FALINI B., HILDRETH P., WOOLSTON R.E., PULFORD K., CORDELL J.L., COHEN B., DE WOLF-PEETERS C. and MASON D.Y. (1983). Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N. Engl. J. Med. 309: 1275-1281.
26. WEI Z.G., LIN T., SUN T.T. and LAVKER R.M. (1997). Clonal analysis of the in vivo differentiation potential of keratinocytes. Invest. Ophthalmol. Vis. Sci. 38: 753-761.
27. YAMAGAMI S., YOKOO S., USUI T., YAMAGAMI H., AMANO S. and EBIHARA N. (2005). Distinct populations of dendritic cells in the normal human donor corneal epithelium. Invest. Ophthalmol. Vis. Sci. 46: 4489-4494.