Spectrophotometric quantification of ascorbic acid contents of fruit and vegetables using the 2,4-dinitrophenylhydrazine method

Journal of Food, Agriculture and Environment

Volumn 5, Issue 3-4, 2007, Pages 165-168

  Al Ani, Majeed ^a   Opara, Linus U ^a   Al Bahri, Dalila ^a   Al Rahbi, Nadia ^a  

^a SULTAN QABOOS UNIVERSITY   (Oman)

Author keywords

2,4 dinitrophenylhydrazine; Ascorbic acid; Fruit and vegetables; Spectrophotometric method; Total vitamin C

Indexed keywords

EID: 36649030602     PISSN: 14590255     EISSN: 14590263     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. th edn. W. Horwitz (Ed.), Chapter 45, pp. 16-20, AOAC International, Office of the Federal Register, U.S. Government, Washington, D.C.
2. Ball, G.F.M. 2006. Vitamins in Foods - Analysis, Bioavailability, and Stability. CRC Taylor & Francis, Boca Raton.
3. Behrens, W.A. and Madere, R. 1987. A highly sensitive high-performance liquid chromatography method for the estimation of ascorbic acid and dehydroascorbic acid in tissues, biological fluids, and foods. Anal. Biochem. 165:102-107.
4. Ferreira, S.L.C., Bandeira, M.L.S., Lemos, V.A., dos Santons, H.C., Spinola Costa, A.C. and de Jesus, D.S. 1997. Sensitive spectrophotometric determination of ascorbic acid in fruit juices and pharmaceutical formulations using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP). Fresenius J. Anal. Chem. 357:1174-1178.
5. rd edn, The Association of Vitamin Chemists, Inc., J. Wiley, Interscience Publishers, New York, pp. 287-344.
6. th edn, Thomson Wadsworth, Belmont, pp. 368-377.
7. Hemandez, Y., Lobo, M.G. and Gonzalez, M. 2006. Determination of vitamin C in tropical fruits: A comparative evaluation of methods. Food Chem. 96:654-664.
8. Hewitt, E.J. and Dickes, G.J. 1961. Spectrophotometric measurements on ascorbic acid and their use for the estimation of ascorbic acid and dehydroascorbic acid in plant tissues. Biochem. J. 78:384-391.
9. Lau, O.W., Luk, S.F. and Wong, K.S. 1986. Background correction method for the determination of ascorbic acid in soft drinks, fruit juices and cordials using direct ultraviolet spectrophotometry. The Analyst 111:665-670.
10. Lee, S.K. and Kader, A.A. 2000. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biol. Technol. 20:207-220.
11. rd edn, W.B. Saunders Co., Philadelphia, pp. 999-1028.
12. Nisperos-Carriedo, M.O., Buslig, B.S. and Shaw, P.E. 1992. Simultaneous detection of dehydroascorbic, ascorbic, and some organic acids in fruits and vegetables by HPLC. J. Agric. Food Chem. 40:1127-1130.
13. Ozyrek, M., Guclu, K., Bektasoglu, B. and Apak, R. 2007. Spectrophotometric determination of ascorbic acid by the modified CUPRAC method with extractive separation of flavonoids-La(III) complexes. Analytica Chimica Acta doi:10.1016/j.aca.2007.01.078 (in press).
14. th edn. W.B. Saunders Co., Philadelphia, pp. 955-1028.
15. Roe, J.H. and Kuether, C.A. 1943. The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J. Biol. Chem. 147:399-407.
16. Schaffert, R.R. and Kingsley, G.R. 1955. A rapid method for the determination of reduced, dehydro-, and ascorbic acid in biological materials. J. Biol. Chem. 212:59-68.
17. Szeto, Y.T., Tomlinson, B. and Benzie, F.F. 2002. Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications of dietary planning and food preservation. Brit. J. Nutr. 87:55-59.
18. Tsumura, F., Oshako, Y., Haraguchi, Y., Kumagai, H., Sakurai, H. and Ishii, K. 1993. Rapid enzymatic assay for ascorbic acid in various foods using peroxidase. J. Food Sci. 58:619-622, 687.
19. Vanderslice, J.T. and Higgs, D.J. 1991. Vitamin C content of foods: Sample variability. Am. J. Clin. Nutr. 54:1323S-1327S.
20. th edn, Thomson Wadsworth, Belmont, pp. 377-385.