Effects of CDB-4022 on leydig cell function in adult male rats

Biology of Reproduction

Volumn 77, Issue 6, 2007, Pages 1017-1026

  Chen, Yu Chyu ^a   Cochrum, Renate K ^a   Tseng, Michael T ^b   Ghooray, Dushan T ^b   Moore, Joseph P ^b   Winters, Stephen J ^a,b   Clark, Barbara J ^a,b  

^a UNIVERSITY OF LOUISVILLE   (United States)

^b University of Louisville School of Medicine   (United States)

Author keywords

Activin; CDB 4022; CYP17A1; Gene regulation; Leydig cells; STAR; Steroid hormones; Steroidogenesis; Testis

Indexed keywords

2 ETHYL 2,3,4,4A,5,9B HEXAHYDRI 8 IODO 7 METHYL 5 CARBOMETHOXYPHENYL 1H INDENO[1,2 C]PYRIDINE; ACTIVIN A; CDB 4022; CHORIONIC GONADOTROPIN; CYCLIC AMP; CYTOCHROME P450 17; INHIBIN B; MESSENGER RNA; PROGESTERONE; PYRIDINE DERIVATIVE; STEROID 17ALPHA MONOOXYGENASE; TESTOSTERONE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CELL FUNCTION; CONTRACEPTION; CONTROLLED STUDY; DRUG EFFECT; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; GENE CONTROL; GONADOTROPIN SECRETING CELL; LEYDIG CELL; MALE; NONHUMAN; PRIORITY JOURNAL; PROGESTERONE BLOOD LEVEL; PROGESTERONE RELEASE; PROTEIN EXPRESSION; RAT; SECRETORY GRANULE; SINGLE DRUG DOSE; TESTOSTERONE BLOOD LEVEL;

ACTIVINS; ANIMALS; CELL LINE, TUMOR; GENE EXPRESSION PROFILING; GONADAL STEROID HORMONES; INDENES; INHIBINS; LEYDIG CELLS; MALE; MICE; PHOSPHOPROTEINS; PIPERIDINES; PITUITARY GLAND; RATS; RATS, SPRAGUE-DAWLEY; RNA; STEROID 17-ALPHA-HYDROXYLASE;

RATTUS;

EID: 36649009935     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.106.059204     Document Type: Article

References (40)

1. Pineau C, Velez de la Calle JF, Pinon-Lataillade G, Jegou B. Assessment of testicular function after acute and chronic irradiation: further evidence for an influence of late spermatids on Sertoli cell function in the adult rat. Endocrinology 1989; 124:2720-2728.
2. Shetty G, Wilson G, Hardy MP, Niu E, Huhtaniemi I, Meistrich ML. Inhibition of recovery of spermatogenesis in irradiated rats by different androgens. Endocrinology 2002; 143:3385-3396.
3. Krawczuk-Rybak M, Solarz E, Wolczynski S. Male gonadal function before and after chemotherapy in prepubertal boys. Rocz Akad Med Bialymst 2004; 49(suppl 1):126-128.
4. Hild SA, Reel JR, Larner JM, Blye RP. Disruption of spermatogenesis and Sertoli cell structure and function by the indenopyridine CDB-4022 in rats. Biol Reprod 2001; 65:1771-1779.
5. Fail PA, Anderson SA, Cook CE. 28-day toxicology test: indenopyridine RTI 4587-056 in male Sprague-Dawley rats. Reprod Toxicol 2000; 14:265-274.
6. Hodel C, Suter K. Reversible inhibition of spermatogenesis with an indenopyridine (20-438). Arch Toxicol Suppl 1978; 1:323-326.
7. Hild SA, Meistrich ML, Blye RP, Reel JR. Lupron depot prevention of antispermatogenic/antifertility activity of the indenopyridine, CDB-4022, in the rat. Biol Reprod 2001; 65:165-172.
8. Hild SA, Attardi BJ, Reel JR. The ability of a gonadotropin-releasing hormone antagonist, acyline, to prevent irreversible infertility induced by the indenopyridine, CDB-4022, in adult male rats: the role of testosterone. Biol Reprod 2004; 71:348-358.
9. Matter BE, Jaeger I, Suter W, Tsuchimoto T, Deyssenroth H. Actions of an antispermatogenic, but non-mutagenic, indenopyridine derivative in mice and Salmonella typhimurium. Mutat Res 1979; 66:113-127.
10. Clark BJ, Stocco DM. Steroidogenic acute regulatory protein: the StAR still shines brightly. Mol Cell Endocrinol 1997; 134:1-8.
11. Clark BJ, Wells J, King SR, Stocco DM. The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR). J Biol Chem 1994; 269:28314-28322.
12. Payne AH, Hales DB. Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr Rev 2004; 25:947-970.
13. Winters SJ, Clark BJ. Testosterone synthesis, transport and metabolism. In: Bremmer WJ (ed.), Contemporary Endocrinology: Androgens in Health and Disease. Totowa, NJ: Humana Press, Inc.; 2003:3-22.
14. Majdic G, McNeilly AS, Sharpe RM, Evans LR, Groome NP, Saunders PT. Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat. Endocrinology 1997; 138:2136-2147.
15. Buzzard JJ, Loveland KL, O'Bryan MK, O'Connor AE, Bakker M, Hayashi T, Wreford NG, Morrison JR, de Kretser DM. Changes in circulating and testicular levels of inhibin A and B and activin A during postnatal development in the rat. Endocrinology 2004; 145:3532-3541.
16. Lin T, Calkins JK, Morris PL, Vale W, Bardin CW. Regulation of Leydig cell function in primary culture by inhibin and activin. Endocrinology 1989; 125:2134-2140.
17. Hsueh AJ, Dahl KD, Vaughan J, Tucker E, Rivier J, Bardin CW, Vale W. Heterodimers and homodimers of inhibin subunits have different paracrine action in the modulation of luteinizing hormone-stimulated androgen biosynthesis. Proc Natl Acad Scei U S A 1987; 84:5082-5086.
18. Drummond AE, Dyson M, Mercer JE, Findlay JK. Differential responses of post-natal rat ovarian cells to FSH and activin. Mol Cell Endocrinol 1996; 122:21-32.
19. Fynn-Thompson E, Cheng H, Teixeira J. Inhibition of steroidogenesis in Leydig cells by Mullerian-inhibiting substance. Mol Cell Endocrinol 2003; 211:99-104.
20. Johnson AL, Bridgham JT, Woods DC. Cellular mechanisms and modulation of activin A- and transforming growth factor beta-mediated differentiation in cultured hen granulosa cells. Biol Reprod 2004; 71:1844-1851.
21. Laurich VM, Trbovich AM, O'Neill FH, Houk CP, Sluss PM, Payne AH, Donahoe PK, Teixeira J. Mullerian inhibiting substance blocks the protein kinase A-induced expression of cytochrome p450 17alpha-hydroxylase/C(17-20) lyase mRNA in a mouse Leydig cell line independent of cAMP responsive element binding protein phosphorylation. Endocrinology 2002; 143:3351-3360.
22. Lejeune H, Chuzel F, Sanchez P, Durand P, Mather JP, Saez JM. Stimulating effect of both human recombinant inhibin A and activin A on immature porcine Leydig cell functions in vitro. Endocrinology 1997; 138:4783-4791.
23. Vanttinen T, Liu J, Kuulasmaa T, Kivinen P, Voutilainen R. Expression of activin/inhibin signaling components in the human adrenal gland and the effects of activins and inhibins on adrenocortical steroidogenesis and apoptosis. J Endocrinol 2003; 178:479-489.
24. Suzuki J, Otsuka F, Inagaki K, Takeda M, Ogura T, Makino H. Novel action of activin and bone morphogenetic protein in regulating aldosterone production by human adrenocortical cells. Endocrinology 2004; 145:639-649.
25. Dooley CA, Attia GR, Rainey WE, Moore DR, Carr BR. Bone morphogenetic protein inhibits ovarian androgen production. J Clin Endocrinol Metab 2000; 85:3331-3337.
26. Klinefelter GR, Hall PF, Ewing LL. Effect of luteinizing hormone deprivation in situ on steroidogenesis of rat Leydig cells purified by a multistep procedure. Biol Reprod 1987; 36:769-783.
27. Ascoli M. Characterization of several clonal lines of cultured Leydig tumor cells: gonadotropin receptors and steroidogenic responses. Endocrinology 1981; 108:88-95.
28. Moore JP Jr, Burger LL, Dalkin AC, Winters SJ. Pituitary adenylate cyclase activating polypeptide messenger RNA in the paraventricular nucleus and anterior pituitary during the rat estrous cycle. Biol Reprod 2005; 73:491-499.
29. Fronhoffs S, Totzke G, Stier S, Wernert N, Rothe M, Bruning T, Koch B, Sachinidis A, Vetter H, Ko Y. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction. Mol Cell Probes 2002; 16:99-110.
30. Auchus RJ, Lee TC, Miller WL. Cytochrome b5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer. J Biol Chem 1998; 273:3158-3165.
31. Nakayama I, Nickerson PA, Skelton FR. An electron microscopic study of the changes in ACTH- and FSH-producing cells during development of methylandrostenediol hypertension in the rat. Am J Pathol 1970; 58:377-401.
32. Ariyaratne HB, Chamindrani Mendis-Handagama S. Changes in the testis interstitium of Sprague-Dawley rats from birth to sexual maturity. Biol Reprod 2000; 62:680-690.
33. Hardy MP, Zirkin BR, Ewing LL. Kinetic studies on the development of the adult population of Leydig cells in testes of the pubertal rat. Endocrinology 1989; 124:762-770.
34. Vergouwen RP, Huiskamp R, Bas RJ, Roepers-Gajadien HL, Davids JA, de Rooij DG. Postnatal development of testicular cell populations in mice. J Reprod Fertil 1993; 99:479-485.
35. Clark BJ, Combs R, Hales KH, Hales DB, Stocco DM. Inhibition of transcription affects synthesis of steroidogenic acute regulatory protein and steroidogenesis in MA-10 mouse Leydig tumor cells. Endocrinology 1997; 138:4893-4901.
36. Imai T, Globerman H, Gertner JM, Kagawa N, Waterman MR. Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Biol Chem 1993; 268:19681-19689.
37. Tseng MT, Kittinger GW, Spies HG. An ultrastructural study of the gonadotrophs in oophorectomized rhesus (Macaca mulatta) adults treated with estrogen. Proc Soc Exp Biol Med 1974; 147:412-417.
38. Koduri S, Hild SA, Pessaint L, Pham T, Reel JR, Attardi BJ. Mechanism of action of 1-CDB-4022, a potential nonhormonal male contraceptive, in the seminiferous epithelium of the rat testis. 89th Annual Meeting of the Endocrine Society 2007; OR36-3.
39. Houk CP, Pearson EJ, Martinelle N, Donahoe PK, Teixeira J. Feedback inhibition of steroidogenic acute regulatory protein expression in vitro and in vivo by androgens. Endocrinology 2004; 145:1269-1275.
40. de Kretser DM. Is spermatogenic damage associated with Leydig cell dysfunction [editorial]? J Clin Endocrinol Metab 2004; 89:3158-3160.