Membrane protein insertion and secretion in bacteria

Methods in Molecular Biology

Volumn 390, Issue , 2007, Pages 17-31

  De Keyzer, Jeanine ^a   Van Der Laan, Martin ^a   Driessen, Arnold J M ^a  

^a NONE

Author keywords

E. coli translocase; Fluorescent imaging; Membrane protein insertion; Protein translocation; SecA; SecYEG; Transcription; Translation

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIUM; BIOLOGICAL MODEL; METABOLISM; PROTEIN PROCESSING; PROTEIN TRANSPORT;

BACTERIA; BACTERIAL PROTEINS; ESCHERICHIA COLI PROTEINS; MEMBRANE TRANSPORT PROTEINS; MODELS, BIOLOGICAL; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 36549072493     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-466-4:17     Document Type: Article

References (16)

1. Osborne, A. R., Rapoport, T. A., and van den Berg, B. (2005) Protein translocation by the Sec61/SecY channel. Annu. Rev. Cell Dev. Biol. 21, 529-550.
2. de Keyzer, J., van der Does, C., and Driessen, A. J. M. (2003) The bacterial translocase: a dynamic protein channel complex. Cell Mol. Life Sci. 60, 2034-2052.
3. Brundage, L., Hendrick, J. P., Schiebel, E., Driessen, A. J., and Wickner, W. (1990) The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 62, 649-657.
4. Economou, A. and Wickner, W. (1994) SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78, 835-843.
5. Hartl, F. U., Lecker, S., Schiebel, E., Hendrick, J. P., and Wickner, W. (1990) The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell 63, 269-279.
6. Valent, Q. A., Scotti, P. A., High, S., et al. (1998) The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J. 17, 2504-2512.
7. H- and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell 64, 927-939.
8. van der Does, C., Manting, E. H., Kaufmann, A., Lutz, M., and Driessen, A. J. M. (1998) Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state. Biochemistry 37, 201-210.
9. Casadaban, M. J. (1976) Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104, 541-555.
10. Baneyx, F. and Georgiou, G. (1990) In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT. J. Bacteriol. 172, 491-494.
11. Kaufmann, A., Manting, E. H., Veenendaal, A. K. J., Driessen, A. J. M., and van der Does, C. (1999) Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE. Biochemistry 38,9115-9125.
12. van der Wolk, J. P., Klose, M., de Wit, J. G., den, B. T., Freudl, R., and Driessen, A. J. M. (1995) Identification of the magnesium-binding domain of the high-affinity ATP-binding site of the Bacillus subtilis and Escherichia coli SecA protein. J. Biol. Chem. 270, 18975-18982.
13. Oliver, D. B. and Beckwith, J. (1981) E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25, 765-772.
14. Manting, E. H., van der Does, C., Remigy, H., Engel, A., and Driessen, A. J. M. (2000) SecYEG assembles into a tetramer to form the active protein translocation channel. EMBO J. 19, 852-861.
15. van der Laan, M., Nouwen, N., and Driessen, A. J. M. (2004) SecYEG proteoliposomes catalyze the ΔΨ-dependent membrane insertion of FtsQ. J. Biol. Chem. 279, 1659-1664.
16. de Keyzer, J., van der Does, C., and Driessen, A. J. M. (2002) Kinetic analysis of the translocation of fluorescent precursor proteins into Escherichia coli membrane vesicles. J. Biol. Chem. 277, 46059-46065.