Expression and characterization of a metalloprotease from a Vibrio parahaemolyticus isolate

Canadian Journal of Microbiology

Volumn 53, Issue 10, 2007, Pages 1168-1173

  Luan, Xiaoyan ^a   Chen, Jixiang ^a   Zhang, Xiao Hua ^a   Li, Yun ^a   Hu, Guobin ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Gelatin activity staining; Vibrio parahaemolyticus; Zinc metalloprotease

Indexed keywords

CHARACTERIZATION; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR MASS; ORGANOMETALLICS; ZINC COMPOUNDS;

GELATIN ACTIVITY STAINING; VIBRIO PARAHAEMOLYTICUS; ZINC METALLOPROTEASE;

ENZYME ACTIVITY;

AMINO ACID; GELATIN; HISTIDINE; METAL; METALLOPROTEINASE; POLYPEPTIDE; RECOMBINANT PROTEIN; VIRULENCE FACTOR;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; PATHOGENICITY; RECOMBINATION; VIRULENCE;

ARTICLE; BACTERIAL VIRULENCE; BINDING AFFINITY; CYTOTOXICITY; ENZYME ACTIVITY; ESCHERICHIA COLI; FISH; GENE; GENE OVEREXPRESSION; GILL; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PATHOGENICITY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; VIBRIO PARAHAEMOLYTICUS; VPM GENE;

ANIMALS; CELLS, CULTURED; CLONING, MOLECULAR; ESCHERICHIA COLI; FISH DISEASES; FLOUNDER; GASTROENTERITIS; GELATIN; GILLS; HUMANS; METALLOPROTEASES; STAINING AND LABELING; VIBRIO INFECTIONS; VIBRIO PARAHAEMOLYTICUS; ZEBRAFISH;

ESCHERICHIA COLI; PLEURONECTOIDEI; VIBRIO PARAHAEMOLYTICUS;

EID: 36549024081     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W07-085     Document Type: Article

References (13)

1. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins using the principle of protein-dye binding. Anal. Biochem. 72: 248-254. doi:10.1016/0003-2697(76)90527-3.
2. Häse, C.C., and Finkelstein, R.A. 1993. Bacterial extracellular zinc-containing metalloproteases. Microbiol. Rev. 57: 823-837.
3. Kim, S.K., Yang, J.Y., and Cha, J. 2002. Cloning and sequence analysis of a novel metalloprotease gene from Vibrio parahaemolyticus 04. Gene, 283: 277-286. doi:10.1016/S0378-1119(01)00882-4.
4. Kreger, A., and Lockwood, D. 1981. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect. Immun. 33: 583-590.
5. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London, U.K.), 227: 680-685. doi:10.1038/227680a0.
6. Lee, J.H., Ahn, S.H., Lee, E.M., Kim, Y.O., Lee, S.J., and Kong, I.S. 2003. Characterization of the enzyme activity of an extracellular metalloprotease (VMC) from Vibrio mimicus and its C-terminal deletions. FEMS Microbiol. Lett. 223: 293-300. doi:10.1016/S0378-1097(03)00401-4.
7. Lin, C., Yu, R.C., and Chou, C.C. 2004. Susceptibility of Vibrio parahaemolyticus to various environmental stresses after cold shock treatment. Int. J. Food Microbiol. 92: 207-215. doi:10.1016/j. ijfoodmicro.2003.10.004.
8. Miyoshi, S., and Shinoda, S. 2000. Microbial metalloproteases and pathogenesis. Microbes Infect. 2: 91-98. doi:10.1016/S1286-4579(00)00280- X.
9. Park, K.S., Ono, T., Rokuda, M., Jang, M.H., Okada, K., Iida, T., and Honda, T. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect. Immun. 72: 6659-6665. doi:10.1128/IAI.72.11.6659-6665.2004.
10. Ross, G.F., Meuth, J., Ohning, B., Kim, Y., and Whitsett, J.A. 1986. Purification of canine surfactant-associated glycoproteins A. Identification of a collagenase-resistant domain. Biochim. Biophys. Acta, 870: 267-278.
11. Sarkar, B.L., Kumar, R., De, S.P., and Pal, S.C. 1987. Hemolytic activity of and lethal toxin production by environmental strains of Vibrio parahaemolyticus. Appl. Environ. Microbiol. 53: 2696-2698.
12. Vallee, B.L., and Auld, D.S. 1990. Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry, 29: 5647-5659. doi:10.1021/bi00476a001.
13. Yu, M.S., Yap, M.N., and Lee, C.Y. 2000. Metal content and biochemical analyses of a recombinant collagenase PrtV from Vibrio parahaemolyticus. Microbiol. Immunol. 44: 805-813.