Characterization of the enzyme activity of an extracellular metalloprotease (VMC) from Vibrio mimicus and its C-terminal deletions

FEMS Microbiology Letters

Volumn 223, Issue 2, 2003, Pages 293-300

  Lee, Jong Hee ^a   Ahn, Sun Hee ^a   Lee, Eun Mi ^a   Kim, Young Ok ^b   Lee, Sang Jun ^b   Kong, In Soo ^a  

^a Pukyong National University   (South Korea)

^b National Fisheries Research and Development Institute (NFRDI)   (South Korea)

Author keywords

C terminal deletion; Collagenase; Metalloprotease; Overexpression; Vibrio mimicus

Indexed keywords

AMINO ACID; BOVINE SERUM ALBUMIN; COLLAGEN; COLLAGENASE; ENZYME; EXODEOXYRIBONUCLEASE III; EXTRACELLULAR METALLOPROTEASE; GELATIN; LACTOFERRIN; LYSOZYME; PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; AFFINITY CHROMATOGRAPHY; ALKALINITY; ARTICLE; BODY TEMPERATURE; CARBOXY TERMINAL SEQUENCE; CELL; CYTOTOXICITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; ESCHERICHIA COLI; FISH; GENE DELETION; GENE OVEREXPRESSION; GENETIC STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; VIBRIO MIMICUS;

BOVINAE; ESCHERICHIA COLI; VIBRIO MIMICUS;

EID: 0038771944     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00401-4     Document Type: Article

References (29)

1. Spira W.M., Fedorka-Cray P.J. Purification of enterotoxins from Vibrio mimicus that appear to be identical to cholera toxin. Infect. Immun. 45:1984;679-684.
2. Nishibuchi M., Seidler R.J. Medium dependent production of extracellular enterotoxin by non-O1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis. Appl. Environ. Microbiol. 45:1983;228-231.
3. Alam M., Miyoshi S., Tomochika K., Shinoda S. Purification and characterization of novel hemagglutinins from Vibrio mimicus: a 39-kilodalton major outermembrane protein and lipopolysaccharide. Infect. Immun. 64:1996;4035-4041.
4. Kang J.H., Lee J.H., Park J.H., Huh S.H., Kong I.S. Cloning and identification of a phospholipase gene from Vibrio mimicus. Biochim. Biophys. Acta. 1394:1998;85-89.
5. Kim G.T., Lee J.Y., Huh S.H., Yu J.H., Kong I.S. Nucleotide sequence of the vmhA gene encoding hemolysin from Vibrio mimicus. Biochim. Biophys. Acta. 1360:1997;102-104.
6. Häse C.C., Finkelstein R.A. Bacterial extracellular zinc containing metalloproteases. Microbiol. Rev. 57:1993;823-837.
7. Finkelstein R.A., Boesman-Finkelstein M., Holt P. Vibrio cholerae hemagglutinin/protease hydrolyzes fibrinonectin and ovomucin: F.M. Burnet revisited. Proc. Natl. Acad. Sci. USA. 80:1983;1092-1095.
8. Chowdhury M.A.R., Miyoshi S.I., Shinoda S. Vascular permeability enhancement by Vibrio mimicus protease and the mechanisms of action. Microbiol. Immunol. 35:1991;1049-1058.
9. Chowdhury M.A.R., Miyoshi S.I., Shinoda S. Purification and characterization of a protease produced by Vibrio mimicus. Infect. Immun. 58:1990;4159-4162.
10. Benitz J.A., Spelbrink R.G., Silva A., Phillips T.E., Stanley C.M., Boesman-Finkelstein M., Finkelstein R.A. Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model. Infect. Immun. 65:1997;3474-3477.
11. Cheng J.C., Shao C.P., Hor L.I. Cloning and nucleotide sequencing of the protease gene of Vibrio vulnificus. Gene. 183:1996;255-257.
12. David V.A., Deutch A.H., Sloma A., Pawlyk D., Ally A., Durham D.R. Cloning, sequencing and expression of the gene encoding the extracellular neutral protease, vibriolysin, of Vibrio proteolyticus. Gene. 112:1992;107-112.
13. Miyoshi S.I., Sonoda Y., Yakiyama H., Rahman M.M., Tomochika K.I., Shinoda S., Yamamoto S., Tobe K. An exocellular thermolysin like metalloprotease produced by Vibrio fluvialis: purification, characterization, and gene cloning. Microb. Pathog. 33:2002;127-134.
14. Miyoshi S., Nakazawa H., Kawata K., Tomochika K., Tobe K. Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease, a member of the thermolysin family. Infect. Immun. 66:1998;4851-4855.
15. Miyoshi S.I., Wakae H., Tomochika K.I., Shinoda S. Functional domain of a zinc metalloprotease from Vibrio vulnificus. J. Bacteriol. 179:1997;7606-7609.
16. Miyoshi S., Kawata K., Tomochika K., Shinoda S., Yamamoto S. The C-terminal domain promotes the hemorrhagic damage caused by Vibrio vulnificus metalloprotease. Toxicon. 39:2001;1883-1886.
17. Lee J.H., Kim G.T., Lee J.Y., Jun H.K., Yu J.H., Kong I.S. Isolation and sequence analysis of metalloprotease gene from Vibrio mimicus. Biochim. Biophys. Acta. 1384:1998;1-6.
18. Lee C.Y., Su S.C., Liaw R.B. Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus. Microbiology. 141:1995;2569-2576.
19. Takeuchi H., Shibano Y., Morihara K., Fukushima J., Inami S., Kell B., Gills A.M., Kawatamoto S., Okuda K. Structural gene and complete amino acid sequence of Vibrio alginolyticus collagenase. Biochem. J. 281:1992;703-708.
20. Norqvist A., Morrman B., Wolf-Wats H. Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum. Infect. Immun. 58:1990;3731-3736.
21. Sasagawa Y., Kamio Y., Matsubara Y., Matsubara Y., Suzuki K., Kojima H., Izaki K. Purification and properties of collagenase from Cytophaga sp. L43-1 strain. Biosci. Biotechnol. Biochem. 57:1993;1894-1898.
22. Philippe B., François L., Maria M., Verneuil B. Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Appl. Environ. Microbiol. 65:1999;2570-2576.
23. Lee J.H., Ahn S.H., Kim S.H., Choi Y.H., Park K.J., Kong I.S. Characterization of Vibrio mimicus phospholipase A (PhlA) and cytotoxicity on fish cell. Biochem. Biophys. Res. Commun. 298:2002;269-276.
24. Yu M.S., Yap M.N., Lee C.Y. Metal content and biochemical analyses of a recombinant collagenase PrtV from Vibrio parahaemolyticus. Microbiol. Immunol. 44:2000;805-813.
25. Kim S.K., Yang J.Y., Cha J.H. Cloning and sequence analysis of a novel metalloprotease gene from Vibrio parahaemolyticus 04. Gene. 283:2002;277-286.
26. Harrington D.J. Bacterial collagenases and collagen degrading enzymes and their potential roles in human disease. Infect. Immun. 64:1996;1885-1891.
27. Milton D.L., Norqvist A., Wolf-Watz H. Cloning of a metalloprotease gene involved in the virulence mechanism of Vibrio anguillarum. J. Bacteriol. 174:1992;7235-7244.
28. Häse C.C., Finkelstein R.A. Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of a HA/protease negative strain. J. Bacteriol. 173:1991;3311-3317.
29. Matsushida O., Jung C.M., Minami J., Katayama S., Nishi N., Okabe A. A study of the collagen binding domain of a 116kDa Clostridium histolyticum collagenase. J. Biol. Chem. 273:1998;3643-3648.