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The magnetic susceptibility of Ademtech 300-nm particles at 48 Oe is 0.05 emu/g, as measured with alternating gradient field magnetometry.
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The magnetic susceptibility of Ademtech 300-nm particles at 48 Oe is 0.05 emu/g, as measured with alternating gradient field magnetometry.
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More information on the correction for the thermal drift on the baseline signal can be found in Supporting Information
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More information on the correction for the thermal drift on the baseline signal can be found in Supporting Information.
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Four different antibodies (S21, S23, S36, S53) were tested in epitope-mapping experiments. We found that S53 as the primary antibody and S36 as the secondary antibody was the pair that gave the most sensitive and specific S100ββ detection (data not shown).
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Four different antibodies (S21, S23, S36, S53) were tested in epitope-mapping experiments. We found that S53 as the primary antibody and S36 as the secondary antibody was the pair that gave the most sensitive and specific S100ββ detection (data not shown).
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It has to be noted that we could couple more then 10 000 RU, but we stopped the coupling manually around 8500 RU to avoid oversaturation of the surface, which could result in hindered S100ββ binding and a drifting baseline.
-
It has to be noted that we could couple more then 10 000 RU, but we stopped the coupling manually around 8500 RU to avoid oversaturation of the surface, which could result in hindered S100ββ binding and a drifting baseline.
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The noise level (N) is calculated as the standard deviation of 10 blank injections. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, while S is reported as the slope of the linear curve.
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The noise level (N) is calculated as the standard deviation of 10 blank injections. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, while S is reported as the slope of the linear curve.
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More information on both particles can be found on the company websites (http://www.ademtech.com and http://www.invitrogen.com). SEM images of both particles are shown in Supporting Information (Figures S3 and S4).
-
More information on both particles can be found on the company websites (http://www.ademtech.com and http://www.invitrogen.com). SEM images of both particles are shown in Supporting Information (Figures S3 and S4).
-
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36448982314
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For the detection of magnetic particles via microscope imaging, the DL can be calculated using the same formula as given in eq 2, with the remark that the units are different compared to SPR sensing. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I are given in percent and S is given in percent mL/ng.
-
For the detection of magnetic particles via microscope imaging, the DL can be calculated using the same formula as given in eq 2, with the remark that the units are different compared to SPR sensing. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I are given in percent and S is given in percent mL/ng.
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For the detection of magnetic particles via the MBS, the units are different compared to SPR sensing. In the case of magnetic bead sensing, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I do not have units and S is given in mL/ng.
-
For the detection of magnetic particles via the MBS, the units are different compared to SPR sensing. In the case of magnetic bead sensing, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I do not have units and S is given in mL/ng.
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