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Volumn 79, Issue 22, 2007, Pages 8669-8677

Magnetic bead sensing platform for the detection of proteins

Author keywords

[No Author keywords available]

Indexed keywords

MAGNETIC BEAD SENSING PLATFORMS; MAGNETOIMMUNO ASSAY;

EID: 36448991211     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac070821n     Document Type: Article
Times cited : (48)

References (55)
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    • Wirix-Speetjens, R.; De Boeck, In, J. Encyclopedia of Sensors; Grimes, C. A., Dickey, E. C., Pishko, M. V., Eds.; American Scientific Publishers: Stevenson Ranch, CA, 2006; 5, pp 403-414.
    • Wirix-Speetjens, R.; De Boeck, In, J. Encyclopedia of Sensors; Grimes, C. A., Dickey, E. C., Pishko, M. V., Eds.; American Scientific Publishers: Stevenson Ranch, CA, 2006; Vol. 5, pp 403-414.
  • 21
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    • Poli-de-Figueiredo, L. F.; Biberthaler, P.; Simao, Filho, C.; Hauser, C.; Mutschler, W.; Jochum, M. Clinics 2006, 61, 41.
    • Poli-de-Figueiredo, L. F.; Biberthaler, P.; Simao, Filho, C.; Hauser, C.; Mutschler, W.; Jochum, M. Clinics 2006, 61, 41.
  • 22
    • 36448970333 scopus 로고    scopus 로고
    • http://www.canag.com/home.htm.
  • 25
    • 36448968041 scopus 로고    scopus 로고
    • The magnetic susceptibility of Ademtech 300-nm particles at 48 Oe is 0.05 emu/g, as measured with alternating gradient field magnetometry.
    • The magnetic susceptibility of Ademtech 300-nm particles at 48 Oe is 0.05 emu/g, as measured with alternating gradient field magnetometry.
  • 30
    • 36448969938 scopus 로고    scopus 로고
    • More information on the correction for the thermal drift on the baseline signal can be found in Supporting Information
    • More information on the correction for the thermal drift on the baseline signal can be found in Supporting Information.
  • 31
  • 33
    • 36448967311 scopus 로고    scopus 로고
    • Four different antibodies (S21, S23, S36, S53) were tested in epitope-mapping experiments. We found that S53 as the primary antibody and S36 as the secondary antibody was the pair that gave the most sensitive and specific S100ββ detection (data not shown).
    • Four different antibodies (S21, S23, S36, S53) were tested in epitope-mapping experiments. We found that S53 as the primary antibody and S36 as the secondary antibody was the pair that gave the most sensitive and specific S100ββ detection (data not shown).
  • 34
    • 36448948136 scopus 로고    scopus 로고
    • It has to be noted that we could couple more then 10 000 RU, but we stopped the coupling manually around 8500 RU to avoid oversaturation of the surface, which could result in hindered S100ββ binding and a drifting baseline.
    • It has to be noted that we could couple more then 10 000 RU, but we stopped the coupling manually around 8500 RU to avoid oversaturation of the surface, which could result in hindered S100ββ binding and a drifting baseline.
  • 37
    • 0003625084 scopus 로고
    • Nakamura, R, Kasahara, Y, Rechnitz, G. A, Eds, American Society for Microbiology: Washington, DC
    • Immunochemical Assap and Biosensor Technologies for the 1990s; Nakamura, R., Kasahara, Y., Rechnitz, G. A., Eds.; American Society for Microbiology: Washington, DC, 1992.
    • (1992) Immunochemical Assap and Biosensor Technologies for the 1990s
  • 38
    • 36448959616 scopus 로고    scopus 로고
    • The noise level (N) is calculated as the standard deviation of 10 blank injections. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, while S is reported as the slope of the linear curve.
    • The noise level (N) is calculated as the standard deviation of 10 blank injections. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, while S is reported as the slope of the linear curve.
  • 43
    • 36448985104 scopus 로고    scopus 로고
    • More information on both particles can be found on the company websites (http://www.ademtech.com and http://www.invitrogen.com). SEM images of both particles are shown in Supporting Information (Figures S3 and S4).
    • More information on both particles can be found on the company websites (http://www.ademtech.com and http://www.invitrogen.com). SEM images of both particles are shown in Supporting Information (Figures S3 and S4).
  • 44
    • 36448982314 scopus 로고    scopus 로고
    • For the detection of magnetic particles via microscope imaging, the DL can be calculated using the same formula as given in eq 2, with the remark that the units are different compared to SPR sensing. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I are given in percent and S is given in percent mL/ng.
    • For the detection of magnetic particles via microscope imaging, the DL can be calculated using the same formula as given in eq 2, with the remark that the units are different compared to SPR sensing. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I are given in percent and S is given in percent mL/ng.
  • 46
    • 11144241809 scopus 로고    scopus 로고
    • Otsuka, H.; Nagasaki, Kataoka, Y., K. Langmuir 2004, 20, 11285.
    • Otsuka, H.; Nagasaki, Kataoka, Y., K. Langmuir 2004, 20, 11285.
  • 50
    • 35348976463 scopus 로고    scopus 로고
    • Fluidic Force Discrimination
    • U.S. Patent 0,253,744, December 16
    • Rife, J. C.; Whitman, L. J. Fluidic Force Discrimination. U.S. Patent 0,253,744, December 16, 2004.
    • (2004)
    • Rife, J.C.1    Whitman, L.J.2
  • 53
    • 36448962561 scopus 로고    scopus 로고
    • For the detection of magnetic particles via the MBS, the units are different compared to SPR sensing. In the case of magnetic bead sensing, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I do not have units and S is given in mL/ng.
    • For the detection of magnetic particles via the MBS, the units are different compared to SPR sensing. In the case of magnetic bead sensing, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ. Both N and I do not have units and S is given in mL/ng.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.