Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex

Journal of Microbiological Methods

Volumn 71, Issue 3, 2007, Pages 343-346

  Raphael, Brian H ^a   Andreadis, Joanne D ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

Clostridium botulinum; Pathogen detection; Real time PCR

Indexed keywords

BOTULINUM TOXIN; HEMAGGLUTININ; NUCLEIC ACID;

ARTICLE; BACTERIAL STRAIN; CLOSTRIDIUM BOTULINUM; CLOSTRIDIUM BUTYRICUM; COMPLEX FORMATION; CONTROLLED STUDY; DNA PURIFICATION; DNA SEQUENCE; DNA TEMPLATE; GENE CLUSTER; HYDROLYSIS; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEROTYPE;

BOTULINUM TOXINS; CLOSTRIDIUM BOTULINUM; DNA, BACTERIAL; GENES, BACTERIAL; HEMAGGLUTININS; NEUROTOXINS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CLOSTRIDIA; CLOSTRIDIUM BARATII; CLOSTRIDIUM BOTULINUM; CLOSTRIDIUM BUTYRICUM;

EID: 36349011431     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.09.016     Document Type: Article

References (12)

1. Arndt J.W., Jacobson M.J., Abola E.E., Forsyth C.M., Tepp W.H., Marks J.D., Johnson E.A., and Stevens R.C. A structural perspective of the sequence variability within botulinum neurotoxin subtypes A1-A4. J. Mol. Biol. 362 (2006) 733-742
2. Braasch D.A., and Corey D.R. Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA. Chem. Biol. 8 (2001) 1-7
3. Centers for Disease Control and Prevention. Botulism in the United States (1899-1996). Handbook for Epidemiologists, Clinicians, and Laboratory Workers (1998), U.S. Department of Health and Human Services, CDC, Atlanta, Ga
4. East A.K., and Collins M.D. Conserved structure of genes encoding components of botulinum neurotoxin complex M and the sequence of the gene coding for the nontoxic component in nonproteolytic Clostridium botulinum type F. Curr. Microbiol. 29 (1994) 69-77
5. Hatheway C.L. Toxigenic clostridia. Clin. Microbiol. Rev. 3 (1990) 66-98
6. Hauser D., Gibert M., Marvaud J.C., Eklund M.W., and Popoff M.R. Botulinal neurotoxin C1 complex genes, clostridial neurotoxin homology, and genetic transfer in Clostridium botulinum. Toxicon 33 (1995) 515-526
7. Henderson I., Whelan S.M., Davis T.O., and Minton N.P. Genetic characterisation of the botulinum toxin complex of Clostridium botulinum strain NCTC 2916. FEMS Microbiol. Lett 140 (1996) 151-158
8. Kools J.J., Ferreira J.L., Maslanka S.E., and Andreadis J.D. Abstr. 42nd Interagency Botulism Research Coordinating Committee Meeting (2005) 17
9. McCroskey L.M., Hatheway C.L., Fenicia L., Pasolini B., and Aureli P. Characterization of an organism that produces type E botulinal toxin but which resembles Clostridium butyricum from the feces of an infant with type E botulism. J. Clin. Microbiol. 23 (1986) 201-202
10. McCroskey L.M., Hatheway C.L., Woodruff B.A., Greenberg J.A., and Jurgenson P. Type F botulism due to neurotoxigenic Clostridium baratii from an unknown source in an adult. J. Clin. Microbiol 29 (1991) 2618-2620
11. Sakaguchi Y., Hayashi T., Kurokawa K., Nakayama K., Oshima K., Fujinaga Y., Ohnishi M., Ohtsubo E., Hattori M., and Oguma K. The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny. Proc. Natl. Acad. Sci. U.S.A. 102 (2005) 17472-17477
12. Shukla H.D., and Sharma S.K. Clostridium botulinum: a bug with beauty and weapon. Crit. Rev. Microbiol. 31 (2005) 11-18