Development of a flow cytometric, non-radioactive cytotoxicity assay in Eisenia fetida: An in vitro system designed to analyze immunosuppression of natural killer-like coelomocytes in response to 7, 12 dimethylbenz[a]anthracene (DMBA)

European Journal of Soil Biology

Volumn 43, Issue SUPPL. 1, 2007, Pages

  Patel, Mukti ^a   Francis, Joseph ^b   Cooper, Edwin L ^c   Fuller Espie, Sheryl L ^a  

^a Cabrini University   (United States)

^b The Master's College   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

DMBA; Flow cytometry; Immunosuppression; In vitro cytotoxicity assay; NK like activity; Non radioactive

Indexed keywords

EISENIA FETIDA;

EID: 36348984060     PISSN: 11645563     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejsobi.2007.08.013     Document Type: Article

References (11)

1. Archuleta M.M., Schieven G.L., Ledbetter J.A., Deanin G.G., and Burchiel S.W. 7,12-Dimethylbenz[a]anthracene activates protein-tyrosine kinases Fyn and Lck in the HPB-ALL human T-cell line and increases tyrosine phosphorylation of phospholipase C-gamma 1, formation of inositol 1,4,5-trisphosphate, and mobilization of intracellular calcium. Proc. Natl. Acad. Sci. USA 90 (1993) 6105-6109
2. Burchiel S., Thompson T., and Davis D. Alterations in mitogen-induced calcium mobilization and intracellular free calcium produced by 7,12 dimethylbenz[a]anthracene in the Jurkat human T cell line. Int. J. Immunopharmacol. 13 (1991) 109-115
3. Cooper E.L., Cossarizza A., Suzuki M.M., Salvioli S., Capri M., Quaglino D., and Franceschi C. Autogeneic but not allogeneic earthworm effector coelomocytes kill the mammalian tumor target K562. Cell. Immunol. 166 (1995) 113-122
4. Cossarizza A., Cooper E.L., Suzuki M.M., Salvioli S., Capri M., Gri G., Quaglino D., and Franceschi C. Earthworm leukocytes that are not phagocytic and cross-react with several human epitopes can kill human tumor cell lines. Exp. Cell Res. 224 (1996) 174-182
5. Hendawi M., Sauve S., Ashour M., Brousseau P., and Fournier M. A new ultrasound protocol for extrusion of coelomocyte cells from the earthworm Eisenia fetida. Ecotoxicol. Environ. Saf. 59 (2004) 17-22
6. Komiyama K., Okaue M., Miki Y., Ohkubo M., Moro I., and Cooper E.L. Non-specific cellular function of Eisenia fetida regulated by polycyclic aromatic hydrocarbons. Pedobiologia 47 (2003) 717-723
7. Kroesen B.J., Mesander G., ter Haar J.G., and The T.H. L. de Leij, Direct visualisation and quantification of cellular cytotoxicity using two colour fluorescence. J. Immunol. Methods 156 (1992) 47-54
8. Ladics G.S., Kawabata T.T., and White Jr. K.L. Suppression of the in vitro humoral immune response of mouse splenocytes by 7,12 dimethylbenz[a]anthracene metabolites and inhibition of immunosuppression by alpha-naphthoflavone. Toxicol. Appl. Pharmacol. 110 (1991) 31-44
9. Suzuki M.M., and Cooper E.L. Spontaneous cytotoxic earthworm leukocytes kill K562 tumor cells. Zool. Sci. 112 (1995) 443-451
10. Thurmond L.M., House R.V., Laurer L.D., and Dean J.H. Suppression of splenic lymphocyte function by 7,12 dimethylbenz[a]anthracene (DMBA) in vitro. Toxicol. Appl. Pharmacol. 93 (1988) 369-377
11. Trochim W.M.K. The t-Test. Retrieved August 16 (2006). http://www.socialresearchmethods.net/kb/stat_t.htm from