Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals

Journal of Medical Microbiology

Volumn 56, Issue 10, 2007, Pages 1309-1316

  Mukherjee, Falguni ^a   Jain, Endra ^a   Patel, Vipul ^a   Nair, Mrinalini ^b  

^a National Dairy Development Board India   (India)

^b M S UNIVERSITY OF BARODA   (India)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME RNA;

ARTICLE; BACTERIAL GENE; BCSP GENE; BLOOD SAMPLING; BRUCELLA; BRUCELLOSIS; CONTROLLED STUDY; DIAGNOSTIC TEST; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; MARKER GENE; NONHUMAN; OMP2 GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEROLOGY;

ANIMALS; ANTIBODIES, BACTERIAL; BIOLOGICAL MARKERS; BRUCELLA; BRUCELLOSIS; CATTLE; DNA, BACTERIAL; DNA, RIBOSOMAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MICE; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 36148988433     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.47160-0     Document Type: Article

References (49)

1. Alton, G. G., Jones, L. M., Angus, R. D. & Verger, J. M. (1988). Techniques for the Brucellosis Laboratory. Paris: Institute National De La Recherche Agronomique.
2. Amin, A. S., Hamdy, M. E. & Ibrahim, A. K. (2001). Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol 83, 37-44.
3. Ariza, J., Pellicer, T., Pallares, R., Foz, A. & Gudiol, F. (1992). Specific antibody profile in human brucellosis. Clin Infect Dis 14, 131-140.
4. Baily, G. G., Krahn, J. B., Drasar, B. S. & Stoker, N. G. (1992). Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95, 271-275.
5. Bardenstein, S., Mandelboim, M., Ficht, T. A., Baum, M. & Banai, M. (2002). Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 gene. J Clin Microbiol 40, 1475-1480.
6. Baumann, P., Furniss, A. L. & Lee, J. V. (1984). Genus I Vibrio Pancini. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 518-538. Edited by N. R. Krieg & J. G. Holt. Baltimore. Williams & Wilkins.
7. Bogdanovich, T., Skurnik, M., Lubeck, P. S., Ahrens, P. & Hoorfar, J. (2004). Validated 5′ nuclease PCR assay for rapid identification of the genus Brucella. J Clin Microbiol 42, 2261-2263.
8. Bricker, B. J. (2002). PCR as a diagnostic tool for brucellosis. Vet Microbiol 90, 435-446.
9. Bricker, B. J. & Ewalt, D. R. (2006). HOOF prints: Brucella strain typing by PCR amplification of multilocus tandem-repeat polymorphisms. Methods Mol Biol 345, 141-173.
10. Bricker, B. J., Tabatabai, L. B., Deyoe, B. L. & Mayfield, J. A. (1988). Conservation of antigenicity in a 31 kDa Brucella protein. Vet Microbiol 18, 313-325.
11. Corbel, M. J. (1997). Brucellosis: an overview. Emerg Infect Dis 3, 213-221.
12. Corbel, M. J. & Brinley-Morgan, W. J. (1984). Genus Brucella Meyer and Shaw 1920, 173. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 377-388. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
13. Cousins, D. V., Williams, S. N., Ross, B. C. & Ellis, T. M. (1993). Use of a repetitive element isolated from Mycobacterium tuberculosis in hybridization studies with Mycobacterium bovis: a new tool for epidemiological studies for bovine tuberculosis. Vet Microbiol 37, 1-17.
14. Da Costa, M., Guillou, J. P., Garin-Bastuji, B., Theibaud, M. & Dubray, G. (1996). Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol 81, 267-275.
15. Dorsch, M., Moreno, E. & Stackebrandt, E. (1989). Nucleotide sequence of the 16S rRNA from Brucella abortus. Nucleic Acids Res 17, 1765.
16. Elfaki, M. G., Uz-Zaman, T., Al-Hokail, A. A. & Nakeeb, S. M. (2005). Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction. Diagn Microbiol Infect Dis 53, 1-7.
17. Ewalt, D. R. & Bricker, B. J. (2000). Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. J Clin Microbiol 38, 3085-3086.
18. Fekete, A., Bantle, J. A. & Halling, S. M. (1992). Detection of Brucella by polymerase chain reaction in bovine fetal and maternal tissues. J Vet Diagn Invest 4, 79-83.
19. Ferrao-Beck, L., Cardoso, R., Munoz, P. M., de Miguel, M. J., Albert, D., Ferreira, A. C., Marin, C. M., Thiebaud, M., Jacques, I. & other authors (2006). Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine. Vet Microbiol 115, 269-277.
20. Fitch, T. A., Bearden, S. W., Sowa, B. A. & Adams, L. G. (1989). DNA sequence and expression of the 36 kilo-dalton outer membrane protein gene of Brucella abortus. Infect Immun 57, 3281-3291.
21. Gee, J. E., De, B. K., Levett, P. N., Whitney, A. M., Novak, R. T. & Popovic, T. (2004). Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. J Clin Microbiol 42, 3649-3654.
22. Godfroid, J., Saegerman, C., Wellemans, V., Walravens, K., Letesson, J. J., Tibor, A., McMillan, A., Spencer, S., Sanna, M. & other authors (2002). How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet Microbiol 90, 461-477.
23. Le Fleche, P., Jacques, I., Grayon, M., Al Dahouk, S., Bouchon, P., Denoeud, F., Nockler, K., Neubauer, H., Guilloteau, L. A. & Vergnaud, G. (2006). Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 6, 9.
24. Leal-Klevezas, D. S., Martinez-Vaquez, I. O., Lopez-Merino, A. & Martinez-Soriano, J. P. (1995). Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol 33, 3087-3090.
25. Leyla, G., Kadri, G. & Umran, O. (2003). Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol 93, 53-61.
26. Lopez-Goni, I. (2001). Detection of Brucella by PCR: application limits and needs. In Brucellosis in Animals and Man. Proceedings of the Combined COST Meeting for Working Groups 1 (Epidemiology), Pamplona, Spain June 11-12, 2001. Progress Report of Action 845: Food & Agriculture Domain. Brussels: European Cooperation in the Field of Scientific and Technical Research (COST).
27. Lulu, A. R., Araz, G. F., Khatib, M. I., Mustafa, M. Y., Yusuf, A. R. & Fenech, F. F. (1988). Human brucellosis in Kuwait: a prospective study of 400 cases. Q J Med 66, 39-54.
28. Marianelli, C., Ciuchini, F., Tarantino, M., Pasquali, P. & Adone, R. (2006). Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping. Microbes Infect 8, 860-865.
29. Mayfield, J. E., Bricker, B. J., Godfrey, H., Crosby, R. M., Knight, D. J., Halling, S. M., Balinsky, D. & Tabataba, L. B. (1988). The cloning, expression, and nucleotide sequence of the BCSP 31 gene from Brucella abortus. Gene 63, 1-9.
30. Morata, P., Quiepo-Ortuno, M. I., Reguera, J. M., Garcia-Ordonez, M. A., Ca'rdenas, A., Juan, D. & Colmenero, J. D. (2003). Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis. J Clin Microbiol 41, 144-148.
31. Mukherjee, F., Jain, J., Grillo, M. J., Blasco, J. M. & Nair, M. (2005). Evaluation of Brucella abortus S19 vaccine strains by bacteriological tests, molecular analysis of ery loci and virulence in BALB/c mice. Biologicals 33, 153-160.
32. Navarro, E., Escribano, J., Fernández, J. A. & Solera, J. (2002). Comparison of three different PCR methods for detection of Brucella spp in human blood samples. FEMS Immunol Med Microbiol 34, 147-151.
33. Navarro, E., Segura, J. C., Castaño, M. J. & Solera, J. (2006). Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin Infect Dis 42, 1266-1273.
34. Nicoletti, P. (1980). The epidemiology of bovine brucellosis. Adv Vet Sci Comp Med 24, 69-89.
35. O'Leary, S., Sheahan, M. & Sweeney, T. (2006). Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res Vet Set 81, 170-176.
36. OIE (1996). WHO, Manual of Standards for Diagnostic Tests and Vaccine, 3rd edn, chapter 3.2.1, pp. 242-255. Paris: Office International des Epizootics.
37. Probert, W. S., Schrader, K. N., Khuong, N. Y., Bystrom, S. L. & Graves, M. H. (2004). Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol 42, 1290-1293.
38. Queipo-Ortuno, M. I., Colmenero, J. D., Reguera, J. M., Garcia-Ordonez, M. A., Pachon, M. E., Gonzalez, M. & Morata, P. (2005). Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. Clin Microbiol Infect 11, 713-718.
39. Queipo-Ortuno, M. I., Morata, P., Ocon, P., Manchado, P. & de Dios Colmenero, J. (2006). Rapid diagnosis of Brucella epididymo-orchitis by real-time polymerase chain reaction assay in urine samples. J Urol 176, 2290-2293.
40. Radostits, O. M., Blood, D. C. & Gay, C. C. (editors) (1994). Veterinary Medicine. A Text Book of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 8th edn, pp. 789-812. London: Bailhere Tindall.
41. Redkar, R., Rose, S., Bricker, B. & DelVecchio, V. (2001). Real-time detection of Brucella abortus, Brucella melitensis, and Brucella suis. Mol Cell Probes 15, 43-52.
42. Rijpens, N. P., Jannes, G., Asbroeck, M. V., Rossau, R. & Herman, L. M. F. (1996). Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Appl Environ Microbiol 62, 1683-1688.
43. Romero, C., Gamazo, C., Pardo, M. & López-Goñi, I. (1995a). Specific detection of Brucella DNA by PCR. J Clin Microbiol 33, 615-617.
44. Romero, C. M., Padro, M., Grillo, M. J., Diaz, R., Blasco, J. M. & Lopez-Goni, I. (1995b). Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in cattle. J Clin Microbiol 33, 3198-3200.
45. Sen, G. P. & Sharma, G. L. (1975). Speciation of seventy-eight Indian strains of Brucella: an epidemiological study. Indian J Anim Sci 45, 537-542.
46. Sifuentes-Rincon, A. M., Revol, A. & Barrera-Saldana, H. A. (1997). Detection and differentiation of the six Brucella species by polymerase chain reaction. Mol Med 3, 734-739.
47. Sreevatsan, S., Bookout, J. B., Ringpis, F., Perumaalla, V. S., Fitch, T. A., Adams, L. G., Haguis, S. D., Elzer, P. H., Bricker, B. J. & other authors (2000). A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle. J Clin Microbiol 38, 2602-2610.
48. Weynants, V., Tibor, A., Denoel, P. A., Saegerman, C., Godfroid, J., Thiange, P. & Letesson, J. J. (1996). Infection of cattle with Yersinia enterocolitica O : 9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet Microbiol 48, 101-112.
49. Yagupsky, P. (1994). Detection of Brucella melitensis by BACTEC NR660 blood culture system. J Clin Microbiol 32, 1899-1901.