Screening for a human single chain Fv antibody against epitope on amyloid-beta 1-40 from a human phage display library

Chinese Medical Journal

Volumn 120, Issue 21, 2007, Pages 1931-1934

  Zhao, Zhen Fu ^a   Gao, Guo Quan ^a   Liu, Shu ^a   Zou, Jun Tao ^a   Xie, Yao ^a   Yuan, Qun Fang ^a   Wang, Hua Qiao ^a   Yao, Zhi Bin ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Alzheimer's disease; Amyloid beta peptide; Phage display library; Single chain Fv antibody

Indexed keywords

AMYLOID BETA PROTEIN[1-40]; EPITOPE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ALZHEIMER DISEASE; AMINO ACID SEQUENCE; ANTIBODY LIBRARY; ANTIGEN BINDING; ARTICLE; B LYMPHOCYTE; BINDING AFFINITY; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; DNA SYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; GENE AMPLIFICATION; HUMAN; HUMAN CELL; IMMUNOTHERAPY; PASSIVE IMMUNIZATION; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN TARGETING; SEQUENCE ANALYSIS;

EID: 36148978941     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.1097/00029330-200711010-00016     Document Type: Article

References (11)

1. Schenk D, Barbour R, Dunn W, Gordon G, Grajeda, H, Guido T, et al. Immunization with amyloid-β attenuates Alzheimer-disease-like pathology in the PDAPP mouse. Nature 1999; 400: 173-177.
2. Dasilva KA, Aubert I, McLaurin J. Vaccine development for Alzheimer's disease. Curr Phirm Des 2006; 12: 4283-4293.
3. Schenk D, Hagen M, Seubert P. Current progress in beta-amyloid immunotherapy Curr Opin Immunol 2004; 16: 599-606.
4. Wilcock DM, DiCarlo G, Henderson D, Jackson J, Clarke K, Ugen KE, et al. Antracranially administered anti-Abeta antibodies reduce beta-amyloid deposition by mechanisms, both independent of and associated with microglial activation. J Neurosci 2003; 23: 3745-3751.
5. Bacskai BJ, Kajdasz-ST, McLellan ME, Games D, Seubert P, Schenk D, et al. Non-Fc-mediated mechanisms are involved in clearance of amyloid-β in vivo by immunotherapy. J Neurosci 2001; 22: 7973-7878.
6. Pfeifer M, Boncristiano S, Bondolfi L, Stalder A, Séller T, Staufenbiel M, et al. Cerebral hemorrhage after passive anti-Aβ immunotherapy. Science 2002; 298: 1379.
7. Munguia, N15, Govezensky T, Martinez R, Manoutcharian K, Gevorkian C. Identification of amyloid-beta 1-42 binding protein fragments by screening of a human brain cDNA library. Neurosci Lett 2006; 307: 79-82.
8. Sblattero D, Bradbury A. A definitive set of oligonucleotide, primers for simplifying human V regions. Immunotechnology 1998; 3: 271-278.
9. Morgan D, Bruce DG. Evidence supporting a role for anti-Aβ antibodies in the treatment of Alzheimer's disease. Neurobiol Aging 2004; 15: 605-608.
10. Duan JZ, Yan XY, Guo XM, Cao WC, Han W, Qi C, et al. A human SARS-CoV neutralizing antibody against-epitope on S2 protein. Biochem Biophys Res Commun 2005; 333: 186-193.
11. Li SB, Wing HQ, Lin X, Xie Y, Yuan QF, Yao ZB. Specific humoral immune responses in rhesus monkeys vaccinated with the Alzheimer's disease-associated β-amyloid 1-15 peptide vaccine. Chin Med J 2005; 118: 660-664.