High-level expression of recombinant active human renin in Sf-9 cells: Rapid purification and characterization

Bioscience, Biotechnology and Biochemistry

Volumn 71, Issue 10, 2007, Pages 2610-2613

  Takahashi, Saori ^a   Hata, Keishi ^a   Kikuchi, Ken Ichi ^b   Gotoh, Takeshi ^b  

^a Forestry and Fisheries Research Center   (Japan)

^b AKITA UNIVERSITY   (Japan)

Author keywords

Baculovirus; Expression; Prorenin; Renin; Sf 9 cells

Indexed keywords

BIOTECHNOLOGY; COLUMN CHROMATOGRAPHY; CULTIVATION; ENZYMES;

CULTURE MEDIUM; HUMAN RENIN; SF-9 CELLS;

CELLS;

COLORING AGENT; RECOMBINANT PROTEIN; RENIN; TRYPAN BLUE;

AMINO ACID SEQUENCE; ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; CELL COUNT; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; TIME;

AMINO ACID SEQUENCE; ANIMALS; BACULOVIRIDAE; CELL COUNT; CELL SURVIVAL; CELLS, CULTURED; COLORING AGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; KINETICS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; RENIN; SPODOPTERA; TIME FACTORS; TRYPAN BLUE;

HEXAPODA;

EID: 36148947900     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70416     Document Type: Article

References (19)

1. Takahashi, S., Ohsawa, T., Miura, R., and Miyake, Y., Purification of high molecular weight (MHW) renin from porcine kidney and direct evidence that HMW renin is a complex of renin with renin binding protein (RnBP). J. Biochem., 93, 265-274 (1983).
2. Inoue, H., Fukui, K., Takahashi, S., and Miyake, Y., Molecular cloning and sequence analysis of a cDNA encoding a porcine kidney renin-binding protein. J. Biol. Chem., 265, 6556-6561 (1990).
3. Takahashi, S., Inoue, H., Fukui, K., and Miyake, Y., Structure and function of renin binding protein. Kidney Int., 46, 1525-1527 (1994).
4. Imai, T., Cho, T., Takamatsu, H., Hori, H., Saito, M., Masuda, T., Hirose, S., and Murakami, K., Synthesis and characterization of human prorenin in Escherichia coli. J. Biochem., 100, 425-432 (1986).
5. Takahashi, S., Ogasawara, H., Watanabe, T., Kumagai, M., Inoue, H., and Hori, K., Refolding and activation of human prorenin expressed in Escherichia coli: application of recombinant human renin for inhibitor screening. Biosci. Biotechnol. Biochem., 70, 2913-2918 (2006).
6. Poorman, R. A., Palermo, D. P., Post, L. E., Murakami, K., Kinner, J. H., Smith, C. W., Reardon, I., and Heinrikson, R. L., Isolation and characterization of native human renin derived from Chinese hamster ovary cells. Proteins, 1, 139-145 (1986).
7. Fritz, L. C., Arfsten, A. E., Dzau, V. J., Atlas, S. A., Baxter, J. D., Fiddes, J. C., Shine, J., Cofer, C. L., Kushner, P., and Ponte, P. A., Characterization of human prorenin expressed in mammalian cells from cloned cDNA. Proc. Natl. Acad. Sci. USA, 83, 4114-4118 (1986).
8. Vlahos, C. J., Walls, J. D., Berg, D. T., and Grinnell, B. W., The purification and characterization of recombinant human renin expressed in the human kidney cell line 293. Biochem. Biophys. Res. Commun., 171, 375-383 (1990).
9. Holzman, T. F., Chung, C. C., Edalji, R., Egan, D. A., Gubbins, E. J., Rueter, A., Howard, G., Yang, L. K., Pederson, T. M., Krafft, G. A., and Wang, G. T., Recombinant human prorenin from CHO cells: expression and purification. J. Protein Chem., 9, 663-672 (1990).
10. Nakagawa, T., Nishiuch, K., Akaki, J., Iwata, H., Satou, R., Suzuki, F., and Nakamura, Y., Efficient production of recombinant human (pro)renin utilizing a decahistidine tag attached at the C-terminus. Biosci. Biotechnol. Biochem., 71, 256-260 (2007).
11. Mathews, S., Döbeli, H., Pruschy, M., Bosser, R., D'Arcy, A., Oefner, C., Zulauf, M., Gentz, R., Breu, V., Matile, H., Schlaeger, J., and Fischli, W., Recombinant human renin produced in different expression systems: biochemical properties and 3D structure. Protein Expr. Purif., 7, 81-91 (1996).
12. Inoue, H., Takahashi, S., and Miyake, Y., Modulation active renin secretion by renin binding protein (RnBP) in mouse pituitary AtT-20 cells transfected with human renin and RnBP cDNAs. J. Biochem., 111, 407-412 (1992).
13. Takahashi, S., Miura, R., Yutani, C., and Miyake, Y., Purification and characterization of human kidney renin. J. Biochem., 99, 307-311 (1986).
14. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
15. Gotoh, T., Miyazaki, Y., Kikuchi, K.-I., and Bentley, W. E., Investigation of sequential behavior of carboxyl proteinase and cysteine protease activities in virus-infected Sf-9 insect cell culture by inhibition assay. Appl. Microbiol. Biotechnol., 56, 742-749 (2001).
16. Takahashi, S., Ushiyama, S., Suzuki, T., Ogawa, K., and Oda, K., Purification and characterization of cysteine proteinase from a baculovirus gene. Biosci. Biotechnol. Biochem., 61, 1507-1511 (1997).
17. Inagami, T., and Murakami, K., Pure renin: isolation from hog kidney and characterization. J. Biol. Chem., 252, 2978-2983 (1977).
18. Imai, T., Miyazaki, H., Hirose, S., Hori, H., Hayashi, T., Kageyama, R., Ohkubo, H., Nakanishi, S., and Murakami, K., Cloning and sequence analysis of cDNA for human renin precursor. Proc. Natl. Acad. Sci. USA, 80, 7405-7409 (1983).
19. Rothwell, V., Kosowski, S., Hadjilambris, O., Baska, R., and Norman, J., Glycosylation of active human renin is necessary for secretion: effect of targeted modifications of Asn-5 and Asn-75. DNA Cell Biol., 12, 291-298 (1993).