Efficient Production of Recombinant Human (Pro)renin Utilizing a Decahistidine Tag Attached at the C-Terminus

Bioscience, Biotechnology and Biochemistry

Volumn 71, Issue 1, 2007, Pages 256-260

  Nakagawa, Tsutomu ^a   Nishiuchi, Kazuhiro ^a   Akaki, Jyunji ^a   Iwata, Hideyuki ^a   Satou, Ryousuke ^a   Suzuki, Fumiaki ^a   Nakamura, Yukio ^a  

^a GIFU UNIVERSITY   (Japan)

Author keywords

Chinese hamster ovary (CHO) cells; Decahistidine tag; Prorenin; Recombinant protein; Renin

Indexed keywords

CATALYSIS; CHROMATOGRAPHY; GROWTH KINETICS; PROTEINS; TISSUE CULTURE;

CHINESE HAMSTER OVARY (CHO) CELLS; DECAHISTIDINE TAG; METAL CHELATE AFFINITY CHROMATOGRAPHY; PRORENIN; RECOMBINANT PROTEIN; RENIN;

CELLS;

RECOMBINANT PROTEIN; RENIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; GENE VECTOR; GENETICS; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHO CELLS; CRICETINAE; CRICETULUS; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RENIN;

CRICETULUS GRISEUS;

EID: 33846539186     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60455     Document Type: Article

References (18)

1. Inagami, T., The renin-angiotensin system. Essays Biochem., 28, 147-164 (1994).
2. Stanton, A., Therapeutic potential of renin inhibitors in the management of cardiovascular disorders. Am. J. Cardiovasc. Drugs, 3, 389-394 (2003).
3. Azizi, M., Webb, R., Nussberger, J., and Hollenberg, N. K., Renin inhibition with aliskiren: where are we now, and where are we going? J. Hypertens., 24, 243-256 (2006).
4. Meek, T. D., Catalytic mechanisms of the aspartic protease. In "Comprehensive Biological Catalysis: A Mechanistic Reference," ed. Sinnott, M., Academic Press, San Diego, pp. 327-344 (1998).
5. Suzuki, F., Nakagawa, T., Kakidachi, H., Murakami, K., Inagami, T., and Nakamura, Y., The dominant role of the prosegment of prorenin in determining the rate of activation by acid or trypsin: studies with molecular chimeras. Biochem. Biophys. Res. Commun., 267, 577-580 (2000).
6. Nguyen, G., Delarue, F., Burckle, C., Bouzhir, L., Giller, T., and Sraer, J.-D., Pivotal role of the renin/prorenin receptor in angiotensin II production and cellular responses to renin. J. Clin. Invest., 109, 1417-1427 (2002).
7. Poorman, R. A., Palermo, D. P., Post, L. E., Murakami, K., Kinner, J. H., Smith, C. W., Reardon, I., and Heinrikson, R. L., Isolation and characterization of native human renin derived from Chinese hamster ovary cells. Proteins, 1, 139-145 (1986).
8. Ishizuka, Y., Shoda, A., Yoshida, S., Kawamura, Y., Haraguchi, K., and Murakami, K., Isolation and characterization of recombinant human prorenin in Chinese hamster ovary cells. J. Biochem., 109, 30-35 (1991).
9. Asselbergs, F. A. M., Rahuel, J., Cumin, F., and Leist, C., Scaled-up production of recombinant human renin in CHO cells for enzymatic and X-ray structure analysis. J. Biotechnol., 32, 191-202 (1994).
10. Pilote, L., McKercher, G., Thibeault, D., and Lamarre, D., Enzymatic characterization of purified recombinant human renin. Biochem. Cell Biol., 73, 163-170 (1995).
11. Mathews, S., Dobeli, H., Pruschy, M., Bosser, R., D'Arcy, A., Oefner, C., Zulauf, M., Gentz, R., Breu, V., Matile, H., Schlaeger, J., and Fischli, W., Recombinant human renin produced in different expression systems: biochemical properties and 3D structure. Protein Expr. Purif., 7, 81-91 (1996).
12. Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol., 60, 523-533 (2003).
13. Nagase, M., Suzuki, F., Fukamizu, A., Takeda, N., Takeuchi, K., Murakami, K., and Nakamura, Y., Sequencing and expression of sheep angiotensinogen cDNA. Biosci. Biotechnol. Biochem., 58, 1884-1885 (1994).
14. Hatsuzawa, K., Kim, W. S., Murakami, K., and Nakayama, K., Purification of mouse Ren 2 prorenin produced in Chinese hamster ovary cells. J. Biochem., 107, 854-857 (1990).
15. Nagase, M., Suzuki, F., Sawai, Y., Orihashi, T., Inui, Y., Nakagawa, T., and Nakamura, Y., Purification and some properties of recombinant sheep angiotensinogen expressed in Chinese hamster ovary cells. Biomed. Res., 18, 439-443 (1997).
16. Suzuki, F., Hatano, Y., Nakagawa, T., Terazawa, K., Gotoh, A., Nasir, U. M., Ishida, Y., and Nakamura, Y., Non-proteolytic activation of human prorenin by antiprorenin prosegment (pf#1: 1P-15P) antiserum. Biosci. Biotechnol. Biochem., 63, 550-554 (1999).
17. Suzuki, F., Takahashi, A., Hyodoh, A., Miyazaki, S., Ishizuka, Y., Murakami, K., and Nakamura, Y., A microplate enzyme-linked immunosorbent assay system for human renin using 3,3′,5,5′-tetramethylbenzidine. Biomed. Res., 13, 321-326 (1992).
18. Nasir, U. M., Takahashi, K., Nagai, T., Nakagawa, T., Suzuki, F., and Nakamura, Y., Two peaks in pH dependence of renin-angiotensinogen reaction. Biosci. Biotechnol. Biochem., 62, 338-340 (1998).