Hypo-phosphorylation leads to nuclear retention of NF-κB p65 due to impaired IκBα gene synthesis

FEBS Letters

Volumn 581, Issue 28, 2007, Pages 5493-5499

  Hochrainer, Karin ^a   Racchumi, Gianfranco ^a   Anrather, Josef ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

I B ; NF B; Nuclear localization; Protein phosphorylation; Transcriptional activity

Indexed keywords

I KAPPA B ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; SYNAPTOTAGMIN I; TRANSCRIPTION FACTOR REL;

ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSOL; EMBRYO; GENE SYNTHESIS; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; MUTANT; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY;

ANIMALS; CELL LINE; CELL NUCLEUS; CYTOSOL; HUMANS; I-KAPPA B KINASE; MICE; MUTATION; PHOSPHORYLATION; SERINE; TRANSCRIPTION FACTOR RELA;

EID: 36049045123     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.10.056     Document Type: Article

References (23)

1. Pahl H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18 (1999) 6853-6866
2. Kumar A., Takada Y., Boriek A.M., and Aggarwal B.B. Nuclear factor-κB: its role in health and disease. J. Mol. Med. 82 (2004) 434-448
3. Beg A.A., Ruben S.M., Scheinman R.I., Haskill S., Rosen C.A., and Baldwin Jr. A.S. I κ B interacts with the nuclear localization sequences of the subunits of NF-κ B: a mechanism for cytoplasmic retention. Genes Dev. 6 (1992) 1899-1913
4. Scherer D.C., Brockman J.A., Chen Z., Maniatis T., and Ballard D.W. Signal-induced degradation of I κ B α requires site-specific ubiquitination. Proc. Natl. Acad. Sci. USA 92 (1995) 11259-11263
5. Traenckner E.B., Pahl H.L., Henkel T., Schmidt K.N., Wilk S., and Baeuerle P.A. Phosphorylation of human I κ B-α on serines 32 and 36 controls I κ B-α proteolysis and NF-κ B activation in response to diverse stimuli. EMBO J. 14 (1995) 2876-2883
6. Sun S.C., Ganchi P.A., Ballard D.W., and Greene W.C. NF-κ B controls expression of inhibitor I κ B α: evidence for an inducible autoregulatory pathway. Science 259 (1993) 1912-1915
7. Arenzana-Seisdedos F., Turpin P., Rodriguez M., Thomas D., Hay R.T., Virelizier J.L., and Dargemont C. Nuclear localization of I κ B α promotes active transport of NF-κ B from the nucleus to the cytoplasm. J. Cell Sci. 110 Pt 3 (1997) 369-378
8. Perkins N.D. Post-translational modifications regulating the activity and function of the nuclear factor κ B pathway. Oncogene 25 (2006) 6717-6730
9. Sakurai H., Chiba H., Miyoshi H., Sugita T., and Toriumi W. IκB kinases phosphorylate NF-κB p65 subunit on serine 536 in the transactivation domain. J. Biol. Chem. 274 (1999) 30353-30356
10. Wang D., Westerheide S.D., Hanson J.L., and Baldwin Jr. A.S. Tumor necrosis factor α-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II. J. Biol. Chem. 275 (2000) 32592-32597
11. Duran A., Diaz-Meco M.T., and Moscat J. Essential role of RelA Ser311 phosphorylation by ζPKC in NF-κB transcriptional activation. EMBO J. 22 (2003) 3910-3918
12. Zhong H., Voll R.E., and Ghosh S. Phosphorylation of NF-κ B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Mol. Cell 1 (1998) 661-671
13. Anrather J., Racchumi G., and Iadecola C. cis-acting element-specific transcriptional activity of differentially phosphorylated nuclear factor-κ B. J. Biol. Chem. 280 (2005) 244-252
14. Beg A.A., Sha W.C., Bronson R.T., Ghosh S., and Baltimore D. Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-κ B. Nature 376 (1995) 167-170
15. Huxford T., Huang D.B., Malek S., and Ghosh G. The crystal structure of the IκBα/NF-κB complex reveals mechanisms of NF-κB inactivation. Cell 95 (1998) 759-770
16. Hoffmann A., Levchenko A., Scott M.L., and Baltimore D. The IκB-NF-κB signaling module: temporal control and selective gene activation. Science 298 (2002) 1241-1245
17. Carlotti F., Dower S.K., and Qwarnstrom E.E. Dynamic shuttling of nuclear factor κ B between the nucleus and cytoplasm as a consequence of inhibitor dissociation. J. Biol. Chem. 275 (2000) 41028-41034
18. Chen L.F., Williams S.A., Mu Y., Nakano H., Duerr J.M., Buckbinder L., and Greene W.C. NF-κB RelA phosphorylation regulates RelA acetylation. Mol. Cell Biol. 25 (2005) 7966-7975
19. Chen L., Fischle W., Verdin E., and Greene W.C. Duration of nuclear NF-κB action regulated by reversible acetylation. Science 293 (2001) 1653-1657
20. Kiernan R., et al. Post-activation turn-off of NF-κ B-dependent transcription is regulated by acetylation of p65. J. Biol. Chem. 278 (2003) 2758-2766
21. Drier E.A., Huang L.H., and Steward R. Nuclear import of the Drosophila Rel protein Dorsal is regulated by phosphorylation. Genes Dev. 13 (1999) 556-568
22. Zhong H., May M.J., Jimi E., and Ghosh S. The phosphorylation status of nuclear NF-κ B determines its association with CBP/p300 or HDAC-1. Mol. Cell 9 (2002) 625-636
23. Anest V., Hanson J.L., Cogswell P.C., Steinbrecher K.A., Strahl B.D., and Baldwin A.S. A nucleosomal function for IκB kinase-α in NF-κB-dependent gene expression. Nature 423 (2003) 659-663