Use of L-buthionine sulfoximine for the efficient expression of disulfide-containing proteins in cell-free extracts of Escherichia coli

Biotechnology and Bioprocess Engineering

Volumn 12, Issue 5, 2007, Pages 574-578

  Oh, In Seok ^a   Kim, Tae Wan ^a   Ahn, Jin Ho ^a   Keum, Jung Won ^a   Choi, Cha Yong ^a   Kim, Dong Myung ^b  

^a Seoul National University   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

Cell free protein synthesis system; Disulfide bond; Glutathione; L buthionine sulfoximine; Recombinant plasminogen activator; S30 extract; Sulfhydryl redox potential

Indexed keywords

BUTHIONINE SULFOXIMINE; CYSTEINE; DISULFIDE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; LIGASE INHIBITOR; RECOMBINANT PLASMINOGEN ACTIVATOR;

ARTICLE; CELL FREE SYSTEM; CHEMICAL REACTION; CONTROLLED STUDY; DISULFIDE BOND; ENZYME INHIBITION; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; NONHUMAN; OXIDATION REDUCTION STATE; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 36049040936     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931357     Document Type: Article

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