An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency

Analytical Biochemistry

Volumn 372, Issue 1, 2008, Pages 21-31

  Mashiyama, Susan T ^a,b   Hansen, Christine M ^c   Roitman, Esther ^a,b   Sarmiento, Stella ^a,b   Leklem, James E ^d   Shultz, Terry D ^c   Ames, Bruce N ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CHILDREN S HOSPITAL OAKLAND RESEARCH INSTITUTE   (United States)

^c WASHINGTON STATE UNIVERSITY   (United States)

^d OREGON STATE UNIVERSITY   (United States)

Author keywords

DNA damage; DNA uracil; Folate; Folic acid; Pyridoxal phosphate; Uracil; Vitamin B6

Indexed keywords

ALKYLATION; AMINO ACIDS; COENZYMES; DNA; GAS CHROMATOGRAPHY; LYMPHOCYTES; MASS SPECTROMETRY;

DNA DAMAGES; FOLATE; FOLIC ACIDS; PYRIDOXAL PHOSPHATES; URACIL; VITAMIN B-6;

AROMATIC COMPOUNDS;

DNA; URACIL;

ADULT; ARTICLE; ASSAY; CONTROLLED STUDY; CYANOCOBALAMIN DEFICIENCY; DIET; HUMAN; HUMAN EXPERIMENT; LYMPHOCYTE; NORMAL HUMAN; OXIDATION; PRIORITY JOURNAL; SMOKING;

EID: 36049028766     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.08.034     Document Type: Article

References (37)

1. Blount B.C., Mack M.M., Wehr C.M., Macgregor J.T., Hiatt R.A., Wang G., Wickramasinghe S.N., Everson R.B., and Ames B.N. Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: Implications for cancer and neuronal damage. Proc. Natl. Acad. Sci. USA 94 (1997) 3290-3295
2. Bailey L.B., Moyers S., and Gregory III J.F. Folate, Present Knowledge in Nutrition. Chapter 21 (2001), ILSI Press, Washington, D.C. pp. 214-229
3. Duthie S.J., and Hawdon A. DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro. FASEB J. 12 (1998) 1491-1497
4. Ames B.N., and Wakimoto P. Are vitamin and mineral deficiencies a major cancer risk?. Nat. Rev. Cancer 2 (2002) 694-704
5. Das K.C., and Herbert V. In vitro DNA synthesis by megaloblastic bone marrow: effect of folates and cobalamins on thymidine incorporation and de novo thymidylate synthesis. Am. J. Hematol. 31 (1989) 11-20
6. Wickramasinghe S.N., and Fida S. Bone marrow cells from vitamin B12- and folate-deficient patients misincorporate uracil into DNA. Blood 83 (1994) 1656-1661
7. Goulian M., Bleile B., and Tseng B.Y. Methotrexate-induced misincorporation of uracil into DNA. Proc. Natl. Acad. Sci. USA 77 (1980) 1956-1960
8. Reidy J.A. Role of deoxyuridine incorporation and DNA repair in the expression of human chromosomal fragile sites. Mutat. Res. 200 (1988) 215-220
9. Luzzatto L., Falusi A.O., and Joju E.A. Uracil in DNA in megaloblastic anemia. N. Engl. J. Med. 305 (1981) 1156-1157
10. Voet D., and Voet J.G. Biochemistry (1990), John Wiley & Sons, New York
11. Lindahl T. Suppression of spontaneous mutagenesis in human cells by DNA base excision-repair. Mutat. Res. 462 (2000) 129-135
12. Dianov G.L., Timchenko T.V., Sinitsina O.I., Kuzminov A.V., Medvedev O.A., and Salganik R.I. Repair of uracil residues closely spaced on the opposite strands of plasmid DNA results in double-strand break and deletion formation. Mol. Gen. Genet. 225 (1991) 448-452
13. Kim Y.I., Pogribny I.P., Basnakian A.G., Miller J.W., Selhub J., James S.J., and Mason J.B. Folate deficiency in rats induces DNA strand breaks and hypomethylation within the p53 tumor suppressor gene. Am. J. Clin. Nutr. 65 (1997) 46-52
14. Crott J.W., Mashiyama S.T., Ames B.N., and Fenech M. The effect of folic acid deficiency and MTHFR C677T polymorphism on chromosome damage in human lymphocytes in vitro. Cancer Epidemiol. Biomarkers Prev. 10 (2001) 1089-1096
15. Leklem J.E. Chapter 18: Vitamin B-6. In: Ziegler E.E., and Filer Jr. L.J. (Eds). Present Knowledge in Nutrition (1996), ILSI Press, Washington, D.C 191-205
16. Institute of Medicine, DRI Reference Intakes for Thiamine, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline., National Academy Press, Washington, DC, 1998.
17. A. Moshfegh, J. Goldman, L. Cleveland, What We Eat in America, NHANES 2001-2002: Usual Nutrient Intakes from Food Compared to Dietary Reference Intakes. U.S. Department of Agriculture, Agricultural Research Service, 2005.
18. Ames B.N. Low micronutrient intake may accelerate the degenerative diseases of aging through allocation of scarce micronutrients by triage. Proc. Natl. Acad. Sci. USA 103 (2006) 17589-17594
19. Ozerol E., Ozerol I., Gokdeniz R., Temel I., and Akyol O. Effect of smoking on serum concentrations of total homocysteine, folate, vitamin B12, and nitric oxide in pregnancy: a preliminary study. Fetal. Diagn. Ther. 19 (2004) 145-148
20. Wallock L.M., Tamura T., Mayr C.A., Johnston K.E., Ames B.N., and Jacob R.A. Low seminal plasma folate concentrations are associated with low sperm density and count in male smokers and nonsmokers. Fertil. Steril. 75 (2001) 252-259
21. Lykkesfeldt J., Christen S., Wallock L.M., Chang H.H., Jacob R.A., and Ames B.N. Ascorbate is depleted by smoking and repleted by moderate supplementation: a study in male smokers and nonsmokers with matched dietary antioxidant intakes. Am. J. Clin. Nutr. 71 (2000) 530-536
22. Fracasso M.E., Doria D., Franceschetti P., Perbellini L., and Romeo L. DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace. Toxicol. Lett. 167 (2006) 131-141
23. Sepaniak S., Forges T., Gerard H., Foliguet B., Bene M.C., and Monnier-Barbarino P. The influence of cigarette smoking on human sperm quality and DNA fragmentation. Toxicology 223 (2006) 54-60
24. Mashiyama S.T., Courtemanche C., Elson-Schwab I., Crott J., Lee B.L., Ong C.N., Fenech M., and Ames B.N. Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytes. Anal. Biochem. 330 (2004) 58-69
25. Antony A.C. Folate receptors. Annu. Rev. Nutr. 16 (1996) 501-521
26. Blount B.C., and Ames B.N. Analysis of uracil in DNA by gas chromatography-mass spectrometry. Anal. Biochem. 219 (1994) 195-200
27. Ramsahoye B.H., Burnett A.K., and Taylor C. Nucleic acid composition of bone marrow mononuclear cells in cobalamin deficiency. Blood 87 (1996) 2065-2070
28. Koury M.J., Horne D.W., Brown Z.A., Pietenpol J.A., Blount B.C., Ames B.N., Hard R., and Koury S.T. Apoptosis of late-stage erythroblasts in megaloblastic anemia: association with DNA damage and macrocyte production. Blood 89 (1997) 4617-4623
29. Crott J.W., Mashiyama S.T., Ames B.N., and Fenech M.F. Methylenetetrahydrofolate reductase C677T polymorphism does not alter folic acid deficiency-induced uracil incorporation into primary human lymphocyte DNA in vitro. Carcinogenesis 22 (2001) 1019-1025
30. Ren J., Ulvik A., Refsum H., and Ueland P.M. Uracil in human DNA from subjects with normal and impaired folate status as determined by high-performance liquid chromatography-tandem mass spectrometry. Anal. Chem. 74 (2002) 295-299
31. Choi S.W., Friso S., Dolnikowski G.G., Bagley P.J., Edmondson A.N., Smith D.E., and Mason J.B. Biochemical and molecular aberrations in the rat colon due to folate depletion are age-specific. J. Nutr. 133 (2003) 1206-1212
32. Beckman K.B., Saljoughi S., Mashiyama S.T., and Ames B.N. A simpler, more robust method for the analysis of 8-oxoguanine in DNA. Free Radical Biol. Med. 29 (2000) 357-367
33. Kretsch M.J., Sauberlich H.E., Skala J.H., and Johnson H.L. Vitamin B-6 requirement and status assessment: young women fed a depletion diet followed by a plant- or animal-protein diet with graded amounts of vitamin B-6. Am. J. Clin. Nutr. 61 (1995) 1091-1101
34. Gregory III J.F. Chemical and nutritional aspects of folate research: analytical procedures, methods of folate synthesis, stability, and bioavailability of dietary folates. Adv. Food Nutr. Res. 33 (1989) 1-101
35. Hansen C.M., Shultz T.D., Kwak H.K., Memon H.S., and Leklem J.E. Assessment of vitamin B-6 status in young women consuming a controlled diet containing four levels of vitamin B-6 provides an estimated average requirement and recommended dietary allowance. J. Nutr. 131 (2001) 1777-1786
36. Kwak H.K., Hansen C.M., Leklem J.E., Hardin K., and Shultz T.D. Improved vitamin B-6 status is positively related to lymphocyte proliferation in young women consuming a controlled diet. J. Nutr. 132 (2002) 3308-3313
37. Ames B.N., Elson-Schwab I., and Silver E.A. High-dose vitamin therapy stimulates variant enzymes with decreased coenzyme binding affinity (increased K(m)): relevance to genetic disease and polymorphisms. Am. J. Clin. Nutr. 75 (2002) 616-658