Comparison of variability associated with sample preparation in two-dimensional gel electrophoresis of cardiac tissue

Journal of Biomolecular Techniques

Volumn 17, Issue 3, 2006, Pages 195-199

  Bland, Alison M ^a   D'Eugenio, Louis R ^a   Dugan, Melissa A ^a   Janech, Michael G ^a   Almeida, Jonas S ^b   Zile, Michael R ^a,c   Arthur, John M ^a,c  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^c RALPH H JOHNSON VA MEDICAL CENTER   (United States)

Author keywords

Heart; Reproducibility; Variability: two dimensional gel electrophoresis

Indexed keywords

ANIMAL; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; HEART MUSCLE; HEART VENTRICLE; PROTEOMICS; RAT; REPRODUCIBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HEART VENTRICLES; MYOCARDIUM; PROTEOMICS; RATS; REPRODUCIBILITY OF RESULTS;

EID: 36049005519     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Klose J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. Humangenetik 1975;26:231-243.
2. O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975;250:4007-4021.
3. Choe LH, Lee KH. Quantitative and qualitative measure of intralaboratory two-dimensional protein gel reproducibility and the effects of sample preparation, sample load, and image analysis. Electrophoresis 2003;24:3500-3507.
4. Terry DE, Desiderio DM. Between-gel reproducibility of the human cerebrospinal fluid proteome. Proteomics 2003;3:1962-1979.
5. Voss T, Haberl P. Observations on the reproducibility and matching efficiency of two-dimensional electrophoresis gels: Consequences for comprehensive data analysis. Electrophoresis 2000;21:3345-3350.
6. Zhan X, Desiderio DM. Differences in the spatial and quantitative reproducibility between two second-dimensional gel electrophoresis systems. Electrophoresis 2003;24:1834-1846.
7. Dowsey AW, Dunn MJ, Yang GZ. The role of bioinformatics in two-dimensional gel electrophoresis. Proteomics 2003;3:1567-1596.
8. Mahon P, Dupree P. Quantitative and reproducible two-dimensional gel analysis using Phoretix 2D Full. Electrophoresis 2001;22:2075-2085.
9. Stanley BA, Neverova I, Brown HA, Van Eyk JE. Optimizing protein solubility for two-dimensional gel electrophoresis analysis of human myocardium. Proteomics 2003;3:815-820.
10. Molloy MP, Brzezinski EE, Hang J, McDowell MT, VanBogelen RA. Overcoming technical variation and biological variation in quantitative proteomics. Proteomics 2003;3:1912-1919.