Single particle refinement in electron crystallography: A pilot study

Journal of Structural Biology

Volumn 160, Issue 3, 2007, Pages 344-352

  Koeck, Philip J B ^a,b,c   Purhonen, Pasi ^a,c   Alvang, Ronny ^a,c   Grundberg, Björn ^a,c   Hebert, Hans ^a,c  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b SÖDERTÖRN UNIVERSITY   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Crystal distortion; Electron crystallography; Membrane proteins; Single particle reconstruction

Indexed keywords

MELIBIOSE; MEMBRANE PROTEIN; PERMEASE;

ARTICLE; CALCULATION; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ELECTRON CRYSTALLOGRAPHY; ELEMENTARY PARTICLE; MEASUREMENT; PILOT STUDY; PRIORITY JOURNAL; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING;

COMPUTER SIMULATION; CRYSTALLIZATION; CRYSTALLOGRAPHY; ESCHERICHIA COLI PROTEINS; FOURIER ANALYSIS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, ELECTRON, TRANSMISSION; MODELS, MOLECULAR; PILOT PROJECTS; PROTEIN CONFORMATION; SYMPORTERS;

EID: 36048997627     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.09.001     Document Type: Article

References (14)

1. Crowther R.A., Henderson R., and Smith J.M. MRC image processing programs. J. Struct. Biol. 116 (1996) 9-16
2. Frank J. New methods for averaging non-periodic objects and distorted crystals in biological electron microscopy. Optik 63 (1982) 67-89
3. Frank J., Chiu W., and Henderson R. Flopping polypeptide chains and Suleika's subtle imperfections: analysis of variations in the electron micrograph of a purple membrane crystal. Ultramicroscopy 49 (1993) 387-396
4. Henderson R., Baldwin J.M., Downing K.H., Lepault J., and Zemlin F. Structure of purple membrane from Halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution. Ultramicroscopy 19 (1986) 147-178
5. Hunte C., Screpanti E., Venturi M., Rimon A., Padan E., and Michel H. Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature 435 (2005) 1197-1202
6. Konstantinides K., and Rasure J.R. The Khoros software development environment for image and signal processing. IEEE Trans. Image Process. 3 (1994) 243-252
7. Ludtke S.J., Baldwin P.R., and Chiu W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128 (1999) 82-97
8. Marabini R., Sorzano C.O.S., Matej S., Fernandez J.J., Carazo J.M., and Herman G.T. 3-D reconstruction of 2-D crystals in real space. IEEE Trans. Image Process. 13 (2004) 549-561
9. Purhonen P., Lundbäck A.-K., Lemonnier R., Leblanc G., and Hebert H. Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy. J. Struct. Biol. 152 (2005) 76-83
10. Saxton W.O., and Baumeister W. The correlation averaging of a regularly arranged bacterial cell envelope protein. J. Microsc. 127 (1982) 127-138
11. Saxton W.O., Duerr R., and Baumeister W. From lattice distortion to molecular distortion: characterising and exploiting crystal deformation. Ultramicroscopy 46 (1992) 287-306
12. Sherman M.B., Soejima T., Chiu W., and van Heel M. Multivariate analysis of single unit cells in electron crystallography. Ultramicroscopy 74 (1998) 179-199
13. Verschoor A., Tivol W.F., and Mannella C.A. Single-particle approaches in the analysis of small 2D crystals of the mitochondrial channel VDAC. J. Struct. Biol. 133 (2001) 254-265
14. Walz T., and Grigorieff N. Electron crystallography of two-dimensional crystals of membrane proteins. J. Struct. Biol. 121 (1998) 142-161