Development of a real-time polymerase chain reaction assay for quantifying Verticillium albo-atrum DNA in resistant and susceptible alfalfa

Phytopathology

Volumn 97, Issue 11, 2007, Pages 1519-1525

  Larsen, R C ^a   Vandemark, G J ^a   Hughes, T J ^b   Grau, C R ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGI; MEDICAGO SATIVA; VERTICILLIUM ALBO-ATRUM;

EID: 36048968470     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-97-11-1519     Document Type: Article

References (37)

1. Alfalfa Council. 2005. Fall dormancy and pest resistance ratings for alfalfa varieties. Alfalfa Council, Kansas City, MO.
2. Atkinson, T. G. 1981. Verticillium wilt of alfalfa: Challenge and opportunity. Can. J. Plant Pathol. 3:266-272.
3. Busbice, T. H., Hill, R. R., and Carnahan, H. L. 1972. Genetics and breeding procedures. Pages 283-314 in: Alfalfa Science and Technology. C. H. Hanson, ed. American Society of Agronomy, Madison, WI.
4. Busch, L. V., Christie, B. R., Smith, E. A., and Boland, G., 1985. Testing alfalfa cultivars for resistance to an alfalfa strain of Verticillium albo-atrum. Can. J. Plant Pathol. 7:203-205.
5. Carder, J. H., and Barbara, D. J. 1991. Molecular variation and restriction fragment length polymorphisms (RFLPs) within and between six species of Verticillium. Mycol. Res. 95:935-942.
6. Christen, A. A. 1982. Demonstration of Verticillium albo-atrum within alfalfa seed. Phytopathology 72:412-414.
7. Christie, B. R., Papadopoulos, Y. A., and Buschet, L. V. 1985. Genetics and breeding for resistance to Verticillium wilt in alfalfa. Can. J. Plant Pathol. 7:206-210.
8. Correll, J. C., Gordon, T. R., and McCain, A. H. 1988. Vegetative compatibility and pathogenicity of Verticillium albo-atrum. Phytopathology 78:1017-1021.
9. Gordon, T. R., Correll, J. C., Gilchrist, D. G., and Martensen, A. N. 1989. Verticillium wilt of alfalfa in California. Plant Dis. 73:18-20.
10. Gossen, B. D., and Jefferson, P. G. 2004. A novel source of resistance to Verticillium wilt in alfalfa. Can. J. Plant Sci. 84:401-404.
11. Graham, J. H., Peaden, R. N., and Evans, D. W. 1977. Verticillium wilt of alfalfa found in the United States. Plant Dis. Rep. 61:337-340.
12. Grau, C. R. 1991. Standard Test: Verticillium wilt resistance. North American Alfalfa Improvement Conference. Online publication. http://www.naaic.org.
13. Grau, C. R., Delwiche, P. A., Norgren, R. L., O'Connell, T. E., and Maxwell, D. P. 1981. Verticillium wilt of alfalfa in Wisconsin. Plant Dis. 65:843-844.
14. Gray, F. A., and Roth, D. A. 1982. Verticillium wilt of alfalfa in Wyoming. Plant Dis. 66:1080.
15. Harper, A. M., and Huang, H. C. 1984. Contamination of insects by the plant pathogen Verticillium albo-atrum in an alfalfa field. Environ. Entomol. 13:117-120.
16. Heid, C. A., Stevens, J., Livak, K. J., and Williams, P. M. 1996. Real time quantitative PCR. Genome Res. 6:986-994.
17. Huang, H. C., Hanna, M. R., and Kokko, E. G. 1985. Mechanisms of seed contamination by Verticillium albo-atrum in alfalfa. Phytopathology 75:482-488.
18. Huang, H. C., Richards, K. W., and Kokko, E. G. 1986. Role of the leaf-cutter bee in dissemination of Verticillium albo-atrum in alfalfa. Phytopathology 76:75-79.
19. Huang, H. C., Harper, A. M., Kokko, E. G., and Howard, R. J. 1983. Aphid transmission of Verticillium albo-atrum to alfalfa. Can. J. Plant Pathol. 5:141-147.
20. Huang, H. C., Acharya, S. N., Hanna, M. R., Kozub, G. C., and Smith, E. G. 1994. Effect of Verticillium wilt on forage yield of alfalfa in southern Alberta. Plant Dis. 78:1181-1184.
21. Kratka, J., and Kudela, V. 1981. Biochemical changes in alfalfa plants inoculated with Verticillium albo-atrum. Phytopathol. Z. 100:289-299.
22. Larsen, R. C., Hollingsworth, C. R., Vandemark, G. J., Gritsenko, M. A., and Gray, F. A. 2002. A rapid method using PCR-based SCAR markers for the detection and identification of Phoma sclerotioides: The cause of brown root rot disease of alfalfa. Plant Dis. 86:928-932.
23. Leath, K. T., and Pennypacker, B. W. 1990. In: Compendium of Alfalfa Diseases. D. L. Stuteville and D. C. Erwin, eds. American Phytopathological Society, St. Paul, MN.
24. Newcombe, A. G, Papadopoulos, Y. A., Robb, J., and Christie, B. R. 1989. The colonization ratio: A measure of pathogen invasiveness and host resistance in Verticillium wilt of alfalfa. Can. J. Bot. 67:365-370.
25. Ostle, B. 1954. Correlation methods. Pages 174-201 in: Statistics in Research. Iowa State College Press, Ames, IA.
26. Page, M. S., Gray, F. A., Legg, D. E., and Kearl, W. G. 1992. Economic impact and management of Verticillium wilt on irrigated alfalfa hay production in Wyoming. Plant Dis. 76:504-508.
27. Papadopoulos, Y. A., Christie, B. R., Boland, G. J., and Busch, L. V. 1990. The use of histochemical analysis and stem colonization for distinguishing reactions of alfalfa to Verticillium wilt. Can. J. Bot. 69:1275-1283.
28. Paran, I., and Michelmore, R. W. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85:985-993.
29. Peaden, R. N., Gilbert, R. G., and Christen, A. A. 1985. Control of Verticillium albo-atrum on alfalfa. Can. J. Plant Pathol. 7:211-214.
30. Sambrook, J., Fritsch, E. F., and Mamatis, T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
31. Smith, E. G., Acharya, S. N., and Huang, H. C. 1995. Economics of growing Verticillium wilt resistant and adapted alfalfa cultivars in Western Canada. Agron. J. 87:1206-1210.
32. Tuite, J. 1969. Plant Pathological Methods. Burgess Publishing Co. Minneapolis, MN.
33. Vandemark, G. J., and Barker, B. M. 2003. Quantifying Phytophthora medicaginis in susceptible and resistant alfalfa with a real-time PCR assay. J. Phytopathol. 151:577-583.
34. Vandemark, G. J., Barker, B. M., and Gritsenko, M. A. 2001. Quantifying Aphanomyces euteiches in alfalfa with a fluorescent polymerase chain reaction assay. Phytopathology 92:265-272.
35. Vandemark, G. J., Larsen, R. C., and Hughes, T. J. 2006. Heritability of resistance to Verticillium wilt in alfalfa. Plant Dis. 90:314-318.
36. Viands, D. R., Lowe, C. C., Bergstrom, G. C., Vaughn, D. L., and Hansen, J. L. 1992. Association of level of resistance to Verticillium will with alfalfa forage yield and stand. J. Prod. Agric. 5:504-509.
37. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingley, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535.