Quantifying Aphanomyces euteiches in alfalfa with a fluorescent polymerase chain reaction assay

Phytopathology

Volumn 92, Issue 3, 2002, Pages 265-272

  Vandemark, G J ^a   Barker, B M ^a   Gritsenko, M A ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APHANOMYCES; APHANOMYCES EUTEICHES; FUNGI; MEDICAGO SATIVA;

EID: 0036197233     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.2002.92.3.265     Document Type: Article

References (38)

1. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants
2. Standard test: Fusarium wilt resistance
3. Genetics and breeding procedures
4. Factors affecting zoospore production by Aphanomyces euteiches
5. Characterization of Aphanomyces euteiches isolates recovered from alfalfa in Wisconsin
6. Heritability
7. Standard test to characterize alfalfa cultivars (Revised 1998): Aphanomyces root rot resistance (races 1 and 2)
8. Aphanomyces root rot
9. Standard test: Verticillium wilt resistance
10. Registration of WAPH-1 alfalfa germplasm with resistance to Aphanomyces root rot
11. Variability in virulence of Aphanomyces euteiches
12. Real time quantitative PCR
13. Detection of specific polymerase chain reaction products by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase
14. Specificity of resistance to Aphanomyces euteiches in seedling alfalfa
15. Evaluation of the forma specialis concept in Aphanomyces euteiches
16. Development of a standardized test for evaluating alfalfa for resistance to Aphanomyces euteiches race 2
17. Development of an antiserum to quantify Aphanomyces euteiches in resistant pea lines
18. Standard test: Columbia root knot nematode resistance
19. Quantification of Aphanomyces euteiches in pea roots using specific fatty acids
20. Improving resistance to Aphanomyces root rot in peas via recurrent selection
21. Advances in quantitative PCR technology
22. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR products and nucleic acid hybridization
23. Characteristics and frequency of Aphanomyces euteiches races 1 and 2 associated with alfalfa in the midwestern United States
24. An evaluation of the excised root tip method for determining the resistance of Pisum sativum to Aphanomyces euteiches
25. Detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay
26. Virulence of Aphanomyces euteiches isolates from Iowa and Wisconsin and benefits of resistance to A. euteiches in alfalfa cultivars
27. Correlation methods
28. Aphanomyces species and their root diseases in pea and sugarbeet
29. Aphanomyces euteiches f. sp. phaseoli, a causal agent of bean root and hypocotyl rot
30. Prevalence and virulence of Phytophthora, Aphanomyces, Pythium, Rhizoctonia, and Fusarium isolated from diseased alfalfa seedlings
31. Detection of potato leaf-roll virus in dormant potato tubers by immunocapture and a fluorogenic 5′ nuclease RT-PCR assay
32. Standard test: Phytophthora root rot resistance
33. A PCR-based assay by sequence-characterized DNA markers for the identification and detection of Aphanomyces euteiches
34. Improved seedling health, yield, and stand persistence with alfalfa resistant to Aphanomyces root rot
35. Alfalfa cultivar performance with differing levels of resistance to Phytophthora and Aphanomyces root rots
36. Root heave of alfalfa cultivars with differing levels of resistance to Aphanomyces root rot
37. Molecular detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds