Monoclonal antibody fluorescence for routine lymphocyte subpopulation analysis with the Abbott CELL-DYN Sapphire haematology analyser

International Journal of Laboratory Hematology

Volumn 29, Issue 6, 2007, Pages 446-453

  Molero, T ^a   Lemes, A ^a   De La Iglesia, S ^a   Scott, C S ^b  

^a HOSPITAL UNIVERSITARIO DE GRAN CANARIA DR NEGRÍN   (Spain)

^b ABBOTT GMBH AND CO KG   (Germany)

Author keywords

Flow cytometry; General haematology; Immunophenotypic analysis; Laboratory automation; Surface markers

Indexed keywords

EDETIC ACID; MONOCLONAL ANTIBODY;

ADOLESCENT; ADULT; AGED; ANALYZER; ARTICLE; B LYMPHOCYTE; BLOOD ANALYSIS; CELL SUBPOPULATION; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; FLUORESCENCE; HUMAN; IMMUNOPHENOTYPING; INFANT; LYMPHOCYTE COUNT; LYMPHOCYTOSIS; NATURAL KILLER CELL; PRIORITY JOURNAL; T LYMPHOCYTE;

ADOLESCENT; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; IMMUNOPHENOTYPING; INFANT; LABORATORIES, HOSPITAL; LYMPHOCYTE COUNT; LYMPHOCYTES; LYMPHOCYTOSIS; MALE;

EID: 35848961652     PISSN: 17515521     EISSN: 1751553X     Source Type: Journal    
DOI: 10.1111/j.1751-553X.2007.00934.x     Document Type: Article

References (11)

1. Chan W.C., Link S., Mawle A., Check I., Byrnes R.K. Winton A.F. (1986) Heterogeneity of large granular lymphocyte proliferations: delineation of two major subtypes. Blood 68, 1142 1153.
2. Gill J.E., Davis K.A., Cowart W.J., Napacena F.U. Kim Y.R. (2000) A rapid and accurate closed tube immunoassay for platelets on an automated hematology analyzer. American Journal of Clinical Pathology 114, 47 56.
3. Hunt K.M., Steed A., Morgan M.A.M., Bynoe A.G., Paparpia L.A. Scott C.S. (1990) Chronic B-cell malignancies in patients with lymphocytosis. Leukemia and Lymphoma 2, 257 258.
4. Johannessen B., Roemer B., Flatmoen L., Just T., Aarsand A.K. Scott C.S. (2006) Implementation of monoclonal antibody fluorescence on the Abbott CELL-DYN Sapphire haematology analyser: evaluation of lymphoid, myeloid and platelet markers. Clinical and Laboratory Haematology 28, 84 96.
5. + T-cell populations on the Cell-Dyn 4000 hematology analyzer. Laboratory Hematology 6, 137 143.
6. Molero T., Roemer B., Del Mar Perera Alvarez M., Lemes A., De La Iglesia Inigo S., Palacios G. Scott C.S. (2005) Analysis and enumeration of T cells, B cells and NK cells using the monoclonal antibody fluorescence capability of a routine haematology analyser (Cell-Dyn CD4000). Clinical and Laboratory Haematology 27, 224 234.
7. Scott C.S. Richards S.J. (1992) Classification of large granular lymphocyte (LGL) and NK-associated (NKa) disorders. Blood Reviews 6, 220 233.
8. Scott C.S., Richards S.J., Sivakumaran M., Short M., Child J.A., Hunt K.M., McEvoy M., Steed A.J., Balfour I.C. Parapia L.A. (1993) Transient and persistent expansions of large granular lymphocytes (LGL) and NK-associated (NKa) cells: The Yorkshire Leukaemia Group study. British Journal of Haematology 83, 505 515.
9. Scott C.S., Richards S.J. Sivakumaran M. (1994a) Disorders of large granular lymphocytes (LGL) and NK-associated (NKa) cells. Blood 83, 301 303.
10. Scott C.S., Richards S.J., Tait T. Jack A.S. (1994b) Large granular lymphocyte expansions in rheumatoid disease. British Journal of Rheumatology 33, 1094 1096.
11. Shearer W.T., Rosenblatt H.M., Gelman R.S., Oyomopito R., Plaeger S., Stiehm E.R., Wara D.W., Douglas S.D., Luzuriaga K., McFarland E.J., Yogev R., Rathore M.H., Levy W., Graham B.L. Spector S.A. (2003) Lymphocyte subsets in healthy children from birth through 18 years of age: the pediatric AIDS clinical Trails Group P1009 study. Journal of Allergy and Clinical Immunology 112, 973 980.