Analysis and enumeration of T cells, B cells and NK cells using the monoclonal antibody fluorescence capability of a routine haematology analyser (Cell-Dyn CD4000)

Clinical and Laboratory Haematology

Volumn 27, Issue 4, 2005, Pages 224-234

  Molero, T ^a   Roemer, B ^b   Del Mar Perera Alvarez, M ^a   Lemes, A ^a   De La Iglesia Inigo S ^a   Palacios, G ^c   Scott, C S ^b  

^a HOSPITAL UNIVERSITARIO DE GRAN CANARIA DR NEGRÍN   (Spain)

^b ABBOTT GMBH AND CO KG   (Germany)

^c ABBOTT LABORATORIES   (Spain)

Author keywords

Cell Dyn CD4000; Lymphocytes; Monoclonal antibodies; Quantitation

Indexed keywords

CD19 ANTIGEN; CD3 ANTIGEN; CD56 ANTIGEN; IA ANTIGEN; MONOCLONAL ANTIBODY;

ARTICLE; B LYMPHOCYTE; BLOOD ANALYSIS; BLOOD SAMPLING; CELL POPULATION; CELL STRUCTURE; FLOW CYTOMETRY; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; LYMPHOCYTE ANALYSIS; LYMPHOCYTE COUNT; NATURAL KILLER CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION;

ANTIBODIES, MONOCLONAL; AUTOANALYSIS; B-LYMPHOCYTES; CELL COUNT; FLUORESCENCE; HEMATOLOGIC TESTS; HUMANS; IMAGE CYTOMETRY; IMMUNOHISTOCHEMISTRY; IMMUNOPHENOTYPING; KILLER CELLS, NATURAL; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES;

EID: 23444443836     PISSN: 01419854     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2257.2005.00695.x     Document Type: Article

References (9)

1. Bland J.M. & Altman D.G. (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet i, 307-310.
2. Erwa W., Bauer F.R., Etschmaier R., Steiner U., Scott C.S. & Sedlmayr P. (1998) Analysis of aged samples with the Abbott CD4000 hematology analyzer. European Journal of Laboratory Medicine 6, 4-15.
3. Kim Y.R., Yee M., Metha S., Chupp V., Kendall R. & Scott C.S. (1998) Simultaneous differentiation and quantitation of erythroblasts and white blood cells on a high throughput clinical haematology analyser. Clinical and Laboratory Haematology 20, 21-29.
4. Marshall P., Hung D., Yuan J. & Kim Y.R. (2000) Rapid, automated, closed-tube quantitation of CD4+ and CD8+ T-cell populations on the Cell-Dyn 4000 hematology analyzer. Laboratory Hematology 6, 137-143.
5. Passing H. & Bablok W. (1983) A new biometrical procedure for testing the equality of measurements from two different analytical methods. Journal of Clinical Chemistry and Biochemistry 21, 709-720.
6. Rothe G. & Schmitz G. (1996) Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Leukemia 10, 877-895.
7. Scott C.S. & Richards S.J. (1992) Classification of large granular lymphocyte (LGL) and NK-associated (NKa) disorders. Blood Reviews 6, 220-233.
8. Tomkinson B., Wagner D., Nelson D. & Sullivan J. (1987) Activated lymphocytes during acute Epstein-Barr virus infection. Journal of Immunology 139, 3202-3207.
9. Van der Meer W., Scott C.S. & de Keijzer M. (2004) Automated flagging influences the inconsistency and bias of band cell and atypical lymphocyte morphological differentials. Clinical Chemistry and Laboratory Medicine 42, 371-377.