Cephalin as an efficient fusogen in hybridoma technology: Can it replace poly ethylene glycol?

Hybridoma

Volumn 26, Issue 5, 2007, Pages 296-301

  Golestani, Reza ^a   Pourfathollah, Ali Akbar ^a,b,c   Moazzeni, Seyed Mohammad ^a,b,c  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b Iranian Blood Research and Fractionation Company   (Iran)

^c IRANIAN BLOOD TRANSFUSION ORGANIZATION   (Iran)

Author keywords

[No Author keywords available]

Indexed keywords

MACROGOL; PHOSPHATIDYLETHANOLAMINE;

ARTICLE; CELL FUSION; CELL VIABILITY; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYBRIDOMA; LYMPHOBLASTOID CELL; MATERIALS TESTING; MYELOMA CELL; PRIORITY JOURNAL; TECHNOLOGY; VIRUS CELL TRANSFORMATION;

ANIMALS; CATTLE; CELL FUSION; CELL LINE; CELL SURVIVAL; HUMANS; HYBRIDOMAS; MICE; PHOSPHATIDYLETHANOLAMINES; POLYETHYLENE GLYCOLS; SAGUINUS;

EID: 35848941169     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2007.0510     Document Type: Article

References (32)

1. Payne WJ Jr, Marshall DL, Shockley RK, and Martin WJ: Clinical laboratory applications of monoclonal antibodies. Clin Microbiol Rev 1988;1:313-329.
2. Kwekkeboom J, de Groot C, and Tager JM: Efficient electric field-induced generation of hybridomas from human B lymphocytes without prior activation in vitro. Hum Antibodies Hybridomas 1992;3:48-53.
3. Panova I, and Gustafsson B: Increased human hybridoma formation by electrofusion of human B cells with heteromyeloma SPAM-8 cells. Hybridoma 1995;14:265-269.
4. Perkins S, Zimmermann U, and Foung SK: Parameters to enhance human hybridoma formation with hypoosmolar electrofusion. Hum Antibodies Hybridomas 1991;2:155-159.
5. Karsten U, Stolley P, Walther I, Papsdorf G, Weber S, Conrad K, Pasternak L, and Kopp J: Direct comparison of electric field-mediated and PEG-mediated cell fusion for the generation of antibody producing hybridomas. Hybridoma 1988;7:627-633.
6. Tomita M, Fukuda T, Ozu A, Kimura K, Tsong TY, and Yoshimura T: Antigen-based immunofluorescence analysis of B-cell targeting: advanced technology for the generation of novel monoclonal antibodies with high efficiency and selectivity. Hybridoma 2006;25:283-292.
7. van Duijn G, Langedijk JP, de Boer M, and Tager JM: High yields of specific hybridomas obtained by electrofusion of murine lymphocytes immunized in vivo or in vitro. Exp Cell Res 1989;183:463-472.
8. Subbiah K, and Ananthanarayanan M: Production of monoclonal antibodies to a tumor-associated antigen by spontaneous cell fusion. Indian J Cancer 2000;37:15-22.
9. Tokunaga T, Chiba J, and Ohnishi K: Attempts to improve hybridoma technology for the production of human monoclonal antibodies. Gan To Kagaku Ryoho 1987;14:2198-2204.
10. Papahadjopoulos D, Poste G, and Schaeffer BE: Fusion of mammalian cells by unilamellar lipid vesicles: influence of lipid surface charge, fluidity and cholesterol. Biochim Biophys Acta 1973;323:23-42.
11. MacDonald RC, Carr VM, MacDonald RI, Carballo PP, and Fisher JA: Interactions between lipid vesicles and cell membranes. Ann NY Acad Sci 1978;308:200-214.
12. Martin FJ, and MacDonald RC: Lipid vesicle-cell interactions. II. Induction of cell fusion. J Cell Biol 1976;70:506-514.
13. Maggio B, Ahkong QF, and Lucy JA: Poly(ethylene glycol), surface potential and cell fusion. Biochem J 1976;158:647-650.
14. Ahkong QF, Fisher D, Tampion W, and Lucy JA: Mechanisms of cell fusion. Nature 1975;253:194-195.
15. Ahkong QF, Fisher D, Tampion W, and Lucy JA: The fusion of erythrocytes by fatty acids, esters, retinol and alpha-tocopherol. Biochem J 1973;136:147-155.
16. Hope MJ, Bruckdorfer KR, Hart CA, and Lucy JA: Membrane cholesterol and cell fusion of hen and guinea-pig erythrocytes. Biochem J 1977;166:255-263.
17. Alkan SS, Mestel F, Jiricka J, and Blaser K: Estimation of heterokaryon formation and hybridoma growth in murine and human cell fusions. Hybridoma 1987;6:371-379.
18. Ohnishi K, Chiba J, Goto Y, and Tokunaga T: Improvement in the basic technology of electrofusion for generation of antibody-producing hybridomas. J Immunol Methods 1987;100:181-189.
19. Ohkohchi N, Itagaki H, Doi H, Taguchi Y, Satomi S, and Satoh S: New technique for producing hybridoma by using laser radiation. Lasers Surg Med 2000;27:262-268.
20. Maggio B, and Lucy JA: Polar-group behaviour in mixed monolayers of phospholipids and fusogenic lipids. Biochem J 1976;155:353-364.
21. Maggio B, and Lucy JA: Studies on mixed monolayers of phospholipids and fusogenic lipids. Biochem J 1975;149:597-608.
22. Papahadjopoulos D, and Poste G: Calcium-induced phase separation and fusion in phospholipid membranes. Biophys J 1975;15:945-948.
23. Li LH, Hensen ML, Zhao YL, and Hui SW: Electrofusion between heterogeneous-sized mammalian cells in a pellet: potential applications in drug delivery and hybridoma formation. Biophys J 1996;71:479-486.
24. Matsuya Y, and Yamane I: Cell hybridization and cell agglutination. I. Enhancement of cell hybridization by lectins. J Cell Sci 1985;78:263-271.
25. Croce CM, Koprowski H, and Eagle H: Effect of environmental pH on the efficiency of cellular hybridization. Proc Natl Acad Sci USA 1972;69:1953-1956.
26. Lane RD, Crissman RS, and Lachman MF: Comparison of polyethylene glycols as fusogens for producing lymphocyte-myeloma hybrids. J Immunol Methods 1984;72:71-76.
27. Lane RD: A short-duration polyethylene glycol fusion technique for increasing production of monoclonal antibody-secreting hybridomas. J Immunol Methods 1985;81:223-228.
28. Kudo T, Saeki H, Saijyo S, Sato N, Numasaki M, and Tachibana T: New strategies to establish human monoclonal antibodies. Tohoku J Exp Med 1992;168:323-327.
29. Olsson L, Kronstrom H, Cambon-De Mouzon A, Honsik C, Brodin T, and Jakobsen B: Antibody producing human-human hybridomas. I. Technical aspects. J Immunol Methods 1983;61:17-32.
30. Butcher RN, Obi TU, and McCullough KC: Rapid isolation of monoclonal hybridoma cultures by a fusion-cloning method: the requirement for aminopterin. Biologicals 1991;19:171-175.
31. Yokoyama WM: Production of monoclonal antibodies. In: Current Protocols in Immunology, 3rd ed. Coligan JE, and Margulies DH (Eds.). John Wiley, New York, 1995, pp. 2.5.1-2.5.17.
32. Jungfer H, Barchet H, and Albert W: Process for the production of permanently culturable animal and human cell lines and the use thereof. US patent # 5,114,847, 1992.