Electrofusion between heterogeneous-sized mammalian cells in a pellet: Potential applications in drug delivery and hybridoma formation

Biophysical Journal

Volumn 71, Issue 1, 1996, Pages 479-486

  Li, L H ^a,b   Hensen, M L ^a   Zhao, Y L ^a   Hui, S W ^a  

^a ROSWELL PARK CANCER INSTITUTE   (United States)

^b CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOPTERIN; BUFFER; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; FLUORESCENT DYE; HYPOXANTHINE; THYMIDINE;

ANIMAL CELL; ARTICLE; CELL CONTACT; CELL FUSION; CELL SWELLING; CELL VIABILITY; CENTRIFUGATION; CHO CELL; COLLOID; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; ELECTRICITY; ERYTHROCYTE; GENE TARGETING; HUMAN; HUMAN CELL; HYBRIDOMA; LEUKEMIA L 1210; LYMPHOCYTE; MOUSE; MYELOMA CELL; NONHUMAN; OSMOSIS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA; MURINAE; MUS;

EID: 0029991784     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79249-4     Document Type: Article

References (25)

1. Abidor, I. G., A. I. Barbal, D. V. Zhelev, P. Poinov, I. N. Bandrina, E. M. Osipova, and S. I. Sukharev. 1993. Electrical properties of cell pellets and cell electrofusion in a centrifuge. Biochim. Biophys. Acta. 1152: 207-218.
2. Abidor, I. G., L, H. Li, and S. W. Hui. 1994a. Studies of cell pellets: I. Electrical properties and porosity. Biophys. J. 67:418-426.
3. Abidor, I. G., L. H. Li, and S. W. Hui. 1994b. Studies of cell pellets: II. Osmotic properties, electroporation, and related phenomena: membrane interactions. Biophys. J. 67:427-435.
4. Abidor, I. G., L. H. Li, and A. E. Sowers. 1994c. Membrane electrofusion yields in membrane fractions obtained with a colloidal-osmotic hemolysis and electrohemolysis procedure. Bioelectrochem. Bioenerget. 34: 31-37.
5. Ahkong, Q. F., D. Fisher, W. Tampion, and J. A. Lucy. 1973. The fusion of erythrocytes by fatty acids, esters, retinol and α-Tocopherol. Biochemical J. 136:147-155.
6. Ahkong, Q. F., D. Fisher, W. Tampion, and J. A. Lucy. 1975. Mechanisms of cell fusion. Nature (London). 253:194-195.
7. Boni, L. T., and S. W. Hui. 1987. Mechanism of polyethylene glycol induced fusion of membranes. In Cell Fusion, A. E. Sowers, editor. Plenum Publishing Corp., New York. 301-330.
8. Furusawa, M., T. Nishimura, M. Yamaizumi, and Y. Okada. 1974. Injection of foreign substances into single cells by cell fusion. Nature (London). 249:449-450.
9. Galfre, G., and C. Milstein. 1981. Preparation of monoclonal antibodies: strategies and procedures. Meth. Enzymol. 73:3-46.
10. Garry, A., G. A. Neil, and U. Zimmermann. 1993. Electrofusion. Meth. Enzymol. 220:174-196.
11. Kao, K. N., and M. R. Michayluk. 1974. A method for high-frequency intergenric fusion of plant protoplast. Planta. 115:355-367.
12. Hui, S. W., T. Isac, L. T. Boni, and A. Sen. 1985. Action of polyethylene glycol on the fusion of human erythrocyte membranes. J. Membr. Biol. 84:137-146.
13. Hui, S. W., and D. A. Stenger. 1993. Electrofusion of cells: comparison of hybridoma productions by electro-fusion and by polyethylene glycol. Meth. Enzymol. 220:212-227.
14. Poster, G., and C. A. Pasternak. 1978. Virus induced cell fusion. In Membrane Fusion, G. Poste and E. L. Nicholson, editors. Elsevier/ North-Holland, Amsterdam. 306-367.
15. Rols, M. P., and J. Tessie. 1992. Experimental evidence for the involvement of the cytoskeleton in mammalian cell electropermeabilization. Biochim. Biophys. Acta. 1111:45-50.
16. Schmitt, J. J., U. Zimmermann, and G. A. Neil. 1988. Efficient generation of stable antibody forming hybridoma cells by electrofusion. Hybridoma. 8:107-115.
17. Song, L. Y., Q. F. Ahkong, J. M. Baldwin, R. O'Reilly, and J. A. Lucy. 1993. Divalent cations, phospholipid asymmetry and osmotic swelling in electrically-induced lysis, cell fusion, and giant cell formation with human erythrocytes. Biochim. Biophys. Acta. 1148:30-38.
18. Sowers, A. E. 1984. Characterization of electric field-induced fusion in erythrocyte ghost membranes. J. Cell Biol. 99:1989-1996.
19. Sowers, A. E. 1988. Fusion events and nonfusion contents mixing events induced in erythrocyte ghosts by an electric pulse. Biophys. J. 54: 619-626.
20. Stenger, D. A., K. V. I. S. Kaler, and S. W. Hui. 1991. Dipole interactions in electrofusion: contributions of membrane potential and effective dipole to interaction pressure. Biophys. J. 59:1074-1084.
21. Stenger, D. A., R. T. Kubinice, W. J. Purucker, H. Liang, and S. W. Hui. 1988. Optimization of electrofusion parameters for efficient production of murine hybridomas. Hybridoma. 7:505-518.
22. Teissie, J., and M. P. Rols. 1993. An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization. Biophys. J. 65:409-413.
23. Vienken, J., and U. Zimmermann. 1985. An improved electrofusion technique for production of mouse hybridoma cells. FEBS Lett. 182: 278-280.
24. Yoshihiro, Y. 1993. Microinjection of macromolecules into cultured cells by erythrocyte ghost-cell fusion. Meth. Enzymol. 221:306-317.
25. Zimmermann, U. 1980. Electric field-mediated fusion and related electrical phenomena. Biochim. Biophys. Acta. 694:227-277.