Hyperspectral image analysis of live cells in various cell cycle stages

Cell Cycle

Volumn 6, Issue 20, 2007, Pages 2563-2570

  Dicker, David T ^a   Lerner, Jeremy M ^b   El Deiry, Wafik S ^a,c  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b LightForm   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Cell cycle; Hyperspectral imaging; Spectral imaging; Spectral library; Spectral signature; Synchronization

Indexed keywords

ARTICLE; CANCER CELL CULTURE; CELL CULTURE; CELL CYCLE; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CONTROLLED STUDY; DNA BINDING; FLUORESCENCE MICROSCOPY; H460 CELL LINE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; PA 1 CELL LINE; SAOS 2 CELL LINE; SPECTROSCOPY; SW480 CELL LINE; U2OS CELL LINE;

EID: 35848936063     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.6.20.4912     Document Type: Article

References (20)

1. Canero A. Targeting the cell cycle for cancer therapy. Br J Cancer 2002; 87:129-33.
2. Vermeulen K, Van Bockstaele DR, Berneman ZN. The cell cycle: A review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif 2003; 36:131-49.
3. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry 1996; 25:1-13.
4. Darzynkiewicz Z, Juan G. DNA Content measurement for DNA ploidy and cell cycle analysis. In: Robinson JP, ed. Current Protocols in Cytometry. New York: John Wiley and Sons, Inc., 1997:7.5.1-24.
5. Smith PJ, Nakeff A, Watson JV. Flow-cytometric detection of changes in the fluorescence emission spectrum of a vital DNA-specific dye in human tumor cells. Exp Cell Res 1985; 159:37-46.
6. Njoh KL, Patterson LH, Zloh M, Wiltshire M, Fisher J, Chapppell S, Ameer-Beg S, Bai Y, Matthews D, Errington RJ, Smith PJ. Spectral analysis of the DNA targeting bisalkylaminoantthraquinone DRAQ5 in intact living cells. Cytometry Part A 2006; 69A:805-16.
7. Kennady KP, Omerod MG, Singh S, Pande G. Variation of mitochondrial size during the cell cycle: A multiparameter flow cytometric and microscopic study. Cytometry 2004; 62A:97-108.
8. Belmont BS. Mitotic chromosome structure and condensation. Curr Opin Cell Biol 2006; 18:632-8.
9. Martinez-Diez M, Santamaria G, Ortega AD, Cuezva JM. Biogenesis and dynamics of mitochondria during the cell cycle: Significance of 3″ UTRs. PLoS ONE 2006; 1:e107.
10. Watson JV, Nakeff A, Chambers SH, Smith PJ. Flow cytometric emission spectrum analysis of Hoechst-33342-stained DNA in chicken thymocytes. Cytometry 1985; 6:310-5.
11. Dobrucki J, Darzynkiewicz Z. Chromatin condensation and sensitivity of DNA in situ to denaturation during cell cycle and apoptosis - A confocal microscopy study. Micron 2001; 32:645-52.
12. Peterson TW, Ibrahim SF, Diercks AH, van den Engh G. Chromatic shifts in the fluorescence emitted by murine thymocytes stianed with Hoechst 33342. Cytometry 2004; 60A:173-81.
13. Tumeh PC, Lerner JM, Dicker DT, El-Deiry WS. Differentiation of vascular and nonvascular skin spectral signatures using in vivo hyperspectral radiometric imaging: Implications for monitoring angiogenesis. Cancer Biol and Ther 2007; 6:447-53.
14. Dicker DT, Lerner J, Van Belle P, Barth SF, DuPont G, Herlyn M, Elder DS, El-Deiry WS. Differentiation of normal and melanoma using high resolution hyperspectral imaging. Cancer Biol and Ther 2006; 5:1033-8.
15. Jin Z, Dicker DT, El-Deiry WS. Enhanced sensitivity of G1 arrested human cancer cells suggests a novel therapeutic strategy using a combination of simvastatin and TRAIL. Cell Cycle 2002; 1:82-9.
16. Lerner JM. Imaging spectrometer fundamentals for researchers in the biosciences - a tutorial. Cytometry 2006; 69A:712-34.
17. Lerner JM, Zucker RM. Calibration and validation of confocal spectral imaging systems. Cytometry 2004; 61A:8-34.
18. Last T, Nugent CD, Owens FJ. Multi component based cross correlation beat detection in electrocardiogram analysis. Biomed Eng Online 2004; 23:26.
19. Lawrence JSP, Masters G. Mapping attenuation beneath North America using waveform cross correlation and cluster analysis. Geophys Res Lett 2006; 33:LO7315.
20. Thomas N. Lighting the circle of life - Fluorescent sensors for covert surveillance of the cell cycle. Cell Cycle 2003; 2:545-9.