Increased CCN2 transcription in keloid fibroblasts requires cooperativity between AP-1 and SMAD binding sites

Annals of Surgery

Volumn 246, Issue 5, 2007, Pages 886-895

  Xia, Wei ^a,b   Kong, Wuyi ^a   Wang, Zhen ^a   Phan, Toan Thang ^c   Lim, Ivor J ^c   Longaker, Michael T ^a   Yang, George P ^a,d  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d VA PALO ALTO HEALTH CARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CCN2 PROTEIN; CONNECTIVE TISSUE GROWTH FACTOR; PROTEIN C JUN; SMAD PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR AP 1; UNCLASSIFIED DRUG;

ADOLESCENT; ADULT; ARTICLE; BINDING SITE; CELLULAR STRESS RESPONSE; CONTROLLED STUDY; ENHANCER REGION; FIBROBLAST; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMEDIATE EARLY GENE; IN VITRO STUDY; KELOID; MECHANICAL STRESS; MICROENVIRONMENT; MOLECULAR MECHANICS; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; SCAR FORMATION; SERUM; STATISTICAL SIGNIFICANCE; STIMULATION; TRANSACTIVATION; WOUND HEALING;

ADOLESCENT; ADULT; BINDING SITES; CELL CULTURE TECHNIQUES; CONNECTIVE TISSUE GROWTH FACTOR; CYSTEINE-RICH PROTEIN 61; FIBROBLASTS; HUMANS; IMMEDIATE-EARLY PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; KELOID; RNA, MESSENGER; SERUM; SMAD PROTEINS, RECEPTOR-REGULATED; TRANSCRIPTION FACTOR AP-1; TRANSFORMING GROWTH FACTOR BETA;

EID: 35649022658     PISSN: 00034932     EISSN: None     Source Type: Journal    
DOI: 10.1097/SLA.0b013e318070d54f     Document Type: Article

References (38)

1. Nemeth AJ. Keloids and hypertrophic scars. J Dermatol Surg Oncol. 1993;19:738-746.
2. Lim IJ, Phan TT, Bay BH, et al. Fibroblasts cocultured with keloid keratinocytes: normal fibroblasts secrete collagen in a keloidlike manner. Am J Physiol Cell Physiol. 2002;283:C212-C222.
3. Lee TY, Chin GS, Kim WJ, et al. Expression of transforming growth factor beta 1, 2, and 3 proteins in keloids. Ann Plast Surg. 1999;43:179-184.
4. Chin GS, Liu W, Peled Z, et al. Differential expression of transforming growth factor-beta receptors I and II and activation of Smad 3 in keloid fibroblasts. Plast Reconstr Surg. 2001;108:423-429.
5. Lau LF, Nathans D. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells. Embo J. 1985;4:3145-3151.
6. Lau LF, Nathans D. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc. Proc Natl Acad Sci USA. 1987;84:1182-1186.
7. Iyer VR, Eisen MB, Ross DT, et al. The transcriptional program in the response of human fibroblasts to serum. Science. 1999;283:83-87.
8. Perbal B. NOV (nephroblastoma overexpressed) and the CCN family of genes: structural and functional issues. Mol Pathol. 2001;54:57-79.
9. Igarashi A, Okochi H, Bradham DM, et al. Regulation of connective tissue growth factor gene expression in human skin fibroblasts and during wound repair. Mol Biol Cell. 1993;4:637-645.
10. Frazier K, Williams S, Kothapalli D, et al. Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. J Invest Dermatol. 1996;107:404-411.
11. Igarashi A, Nashiro K, Kikuchi K, et al. Connective tissue growth factor gene expression in tissue sections from localized scleroderma, keloid, and other fibrotic skin disorders. J Invest Dermatol. 1996;106:729-733.
12. Colwell AS, Phan TT, Kong W, et al. Hypertrophic scar fibroblasts have increased connective tissue growth factor expression after transforming growth factor-beta stimulation. Plast Reconstr Surg. 2005;116:1387-1390; discussion 1391-1392.
13. Khoo YT, Ong CT, Mukhopadhyay A, et al. Upregulation of secretory connective tissue growth factor (CTGF) in keratinocyte-fibroblast coculture contributes to keloid pathogenesis. J Cell Physiol. 2006;208:336-343.
14. Mori T, Kawara S, Shinozaki M, et al. Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: a mouse fibrosis model. J Cell Physiol. 1999;181:153-159.
15. Abreu JG, Ketpura NI, Reversade B, et al. Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta. Nat Cell Biol. 2002;4:599-604.
16. Abe M, Harpel JG, Metz CN, et al. An assay for transforming growth factor-beta using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Anal Biochem. 1994;216:276-284.
17. Chen CC, Mo FE, Lau LF. The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts. J Biol Chem. 2001;276:47329-47337.
18. Kireeva ML, Latinkic BV, Kolesnikova TV, et al. Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development. Exp Cell Res. 1997;233:63-77.
19. Grzeszkiewicz TM, Kirschling DJ, Chen N, et al. CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain. J Biol Chem. 2001;276:21943-21950.
20. Ryseck RP, Macdonald-Bravo H, Mattei MG, et al. Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein. Cell Growth Differ. 1991;2:225-233.
21. Prakash S, Robbins PW, Wyler DJ. Cloning and analysis of murine cDNA that encodes a fibrogenic lymphokine, fibrosin. Proc Natl Acad Sci USA. 1995;92:2154-2158.
22. Denton CP, Abraham DJ. Transforming growth factor-beta and connective tissue growth factor: key cytokines in scleroderma pathogenesis. Curr Opin Rheumatol. 2001;13:505-511.
23. Leask A, Sa S, Holmes A, et al. The control of ccn2 (ctgf) gene expression in normal and scleroderma fibroblasts. Mol Pathol. 2001;54:180-183.
24. Kovary K, Bravo R. Existence of different Fos/Jun complexes during the G0-to-G1 transition and during exponential growth in mouse fibroblasts: differential role of Fos proteins. Mol Cell Biol. 1992;12:5015-5023.
25. Lallemand D, Spyrou G, Yaniv M, et al. Variations in Jun and Fos protein expression and AP-1 activity in cycling, resting and stimulated fibroblasts. Oncogene. 1997;14:819-830.
26. Rozek D, Pfeifer GP. In vivo protein-DNA interactions at the c-jun promoter in quiescent and serum-stimulated fibroblasts. J Cell Biochem. 1995;57:479-487.
27. Leask A, Holmes A, Black CM, et al. Connective tissue growth factor gene regulation: requirements for its induction by transforming growth factor-beta 2 in fibroblasts. J Biol Chem. 2003;278:13008-13015.
28. Holmes A, Abraham DJ, Sa S, et al. CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling. J Biol Chem. 2001;276:10594-10601.
29. Latinkic BV, O'Brien TP, Lau LF. Promoter function and structure of the growth factor-inducible immediate early gene cyr61. Nucleic Acids Res. 1991;19:3261-3267.
30. Latinkic BV, Mo FE, Greenspan JA, et al. Promoter function of the angiogenic inducer Cyr61gene in transgenic mice: tissue specificity, inducibility during wound healing, and role of the serum response element. Endocrinology. 2001;142:2549-2557.
31. Derynck R, Zhang YE. Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature. 2003;425:577-584.
32. Zhang Y, Feng XH, Derynck R. Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Nature. 1998;394:909-913.
33. Qing J, Zhang Y, Derynck R. Structural and functional characterization of the transforming growth factor-beta -induced Smad3/c-Jun transcriptional cooperativity. J Biol Chem. 2000;275:38802-38812.
34. Verrecchia F, Vindevoghel L, Lechleider RJ, et al. Smad3/AP-1 interactions control transcriptional responses to TGF-beta in a promoter-specific manner. Oncogene. 2001;20:3332-3340.
35. Norwitz ER, Xu S, Xu J, et al. Direct binding of AP-1 (Fos/Jun) proteins to a SMAD binding element facilitates both gonadotropin-releasing hormone (GnRH)- and activin-mediated transcriptional activation of the mouse GnRH receptor gene. J Biol Chem. 2002;277:37469-37478.
36. Hall MC, Young DA, Waters JG, et al. The comparative role of activator protein 1 and Smad factors in the regulation of Timp-1 and MMP-1 gene expression by transforming growth factor-beta 1. J Biol Chem. 2003;278:10304 -10313.
37. Rodriguez-Pascual F, Redondo-Horcajo M, Lamas S. Functional cooperation between Smad proteins and activator protein-1 regulates transforming growth factor-beta-mediated induction of endothelin-1 expression. Circ Res. 2003;92:1288-1295.
38. Wang Z, Fong KD, Phan TT, et al. Increased transcriptional response to mechanical strain in keloid fibroblasts due to increased focal adhesion complex formation. J Cell Physiol. 2006;206:510-517.