Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells

Toxicology in Vitro

Volumn 21, Issue 7, 2007, Pages 1258-1261

  Lawton, M ^a   Iqbal, M ^a   Kontovraki, M ^a   Lloyd Mills, C ^a   Hargreaves, A J ^a  

^a NOTTINGHAM TRENT UNIVERSITY   (United Kingdom)

Author keywords

Mercury; Neurite outgrowth; Thimerosal; Tubulin tyrosination

Indexed keywords

METHYLMERCURIC CHLORIDE; THIOMERSAL;

ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; DENSITOMETRY; GLIOMA CELL; MICROTUBULE; MOUSE; NERVE FIBER GROWTH; NEUROBLASTOMA CELL; NONHUMAN; RAT;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE, TUMOR; GLIOMA; METHYLMERCURY COMPOUNDS; MICE; MICROTUBULE PROTEINS; NEURITES; NEUROBLASTOMA; RATS; THIMEROSAL; TUBULIN; TYROSINE;

RATTUS;

EID: 35648954600     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2007.03.018     Document Type: Article

References (25)

1. Atchison W.D., and Hare M.F. Mechanisms of methylmercury-induced neurotoxicity. FASEB J. 8 (2004) 622-629
2. Barra H.S., Arce C.A., and Argarana C.E. Post-translational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 2 (1988) 133-153
3. Cambray-Deakin M.A. Cytoskeleton of the growing axon. In: Burgoyne R.D. (Ed). The Neuronal Cytoskeleton (1999), Wiley-Liss, Inc., New York 233-255
4. Castoldi A.F. Neurotoxicity and molecular effects of methylmercury. Brain Res. Bull. 55 (2001) 197-203
5. Counter S.A., and Buchanan L.H. Mercury exposure in children: a review. Toxicol. Appl. Pharmacol. 198 (2004) 209-230
6. Denizot F., and Lang R. Rapid colorimetric assay for cell growth and survival - modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Meth. 89 (1986) 271-277
7. Dringen R., Kussmaul L., Gutterer J.M., Hirrlinger J., and Hamprecht B. The glutathione system of peroxide detoxification is less efficient in neurons than in astroglial cells. J. Neurochem. 72 (1999) 2523-2530
8. Erck C., Peris L., Andrieux A., Meissirel C., Pointu H., Bosc C., Salin P.A., Job D., and Wehland J. A vital role of tubulin-tyrosine-ligase for neuronal organization. Proc. Natl. Acad. Sci. USA 102 (2005) 7853-7858
9. Flaskos J., McLean W.G., Fowler M.J., and Hargreaves A.J. Tricresyl phosphate inhibits the formation of axon-like processes and disrupts neurofilaments in cultured mouse N2a and rat PC12 cells. Neurosci. Lett. 242 (1998) 101-104
10. Goldman L.R., and Shannon M.W. Technical report: mercury in the environment: implications for pediatricians. Pediatrics 108 (2001) 197-205
11. Graff R.D., Falconer M.M., Brown D.L., and Reuhl K.R. Altered sensitivity of post-translationally modified microtubules to methylmercury in differentiating embryonal carcinoma-derived neurons. Toxicol. Appl. Pharmacol. 144 (1997) 215-224
12. 2+ on human neuronal N- and R-type high-voltage activated calcium channels transiently expressed in human embryonic kidney 293 cells. J. Pharmacol. Exp. Ther. 306 (2003) 1129-1136
13. Hargreaves A.J. The cytoskeleton as a target in cell toxicity. Adv. Mol. Cell Biol. 20 (1997) 119-144
14. Heidemann S.R., Lamoureux P., and Atchison W.D. Inhibition of axonal morphogenesis by nonlethal submicromolar concentrations of methyl mercury. Toxicol. Appl. Pharmacol. 174 (2001) 49-59
15. Jacobson J.L. Contending with contradictory data in a risk assessment context: the case of methylmercury. Neurotoxicology 22 (2001) 667-675
16. James S.J., Slikker III W., Melnyk S., New E., Pogribna M., and Jernigan S. Thimerosal neurotoxicity is associated with glutathione depletion: protection with glutathione precursors. Neurotoxicology 26 (2005) 1-8
17. 4. Nature 227 (1970) 680-685
18. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
19. Miura K., Koide N., Himeno S., Nakagawa I., and Imura M. The involvement of microtubular disruption in methylmercury-induced apoptosis in neuronal and non-neuronal cell lines. Toxicol. Appl. Pharmacol. 160 (1999) 279-288
20. Olivieri G., Brack C., Müller-Spahn F., Stähelin H.B., Herrmann M., Renard P., Brockhaus M., and Hock C. Mercury induces cell cytotoxicity and oxidative stress and increases β-amyloid secretion and tau phosphorylation in SHSY5Y neuroblastoma cells. J. Neurochem. 74 (2000) 231-236
21. O'Kusky J.R. The neurotoxicity of mercury in the developing nervous system. In: Isaacson R.L., and Jensen K.F. (Eds). The Vulnerable Brain and Environmental Risks vol. 2 (1992), Plenum Press, New York 19-34
22. Parran D.R., Barone Jr. S., and Mundy W.R. Methyl mercury decreases NGF-induced TrKa autophosphorylation and neurite outgrowth in PC12 cells. Dev. Brain Res. 141 (2003) 71-81
23. Towbin S., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354
24. 2+ concentration of Rat cerebellar neurons. Toxicology 195 (2004) 77-84
25. Webster D.R., Gundersen G.G., Bulinski J.L., and Borisy G.G. Differential turnover of tyrosinated and detyrosinated microtubules. Proc. Natl. Acad. Sci. USA 84 (1987) 9040-9044