A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate

Analytical Biochemistry

Volumn 371, Issue 2, 2007, Pages 201-207

  Hassiepen, Ulrich ^a   Eidhoff, Ulf ^a   Meder, Gabriele ^a   Bulber, Jean Francois ^a   Hein, Andreas ^a   Bodendorf, Ursula ^a   Lorthiois, Edwige ^a   Martoglio, Bruno ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Nedd8; Protease assay; Rhodamine; SUMO; Ubiquitin specific proteases

Indexed keywords

AMINO ACIDS; FLUORESCENCE; OPTICAL PROPERTIES;

NEDD8; PROTEASE ASSAY; RHODAMINE; SUMO; UBIQUITIN;

PROTEINS;

GLYCINE; OXYGENASE; PROTEINASE; RHODAMINE 110; UBIQUITIN; UBIQUITIN CARBOXYTERMINAL HYDROLASE L3; UBIQUITIN SPECIFIC PROTEASE 2; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; MONITORING; PRIORITY JOURNAL; PROTEIN MODIFICATION; SENSITIVITY AND SPECIFICITY; UBIQUITINATION;

EUKARYOTA;

EID: 35548991428     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.07.034     Document Type: Article

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