A rapid semi automated method for DNA extraction from dried-blood spots: Application to the HLA-DR shared epitope analysis in rheumatoid arthritis

Journal of Immunological Methods

Volumn 328, Issue 1-2, 2007, Pages 220-225

  Pachot, Alexandre ^a,b   Barbalat, Véronique ^a,b   Marotte, Hubert ^a,b   Diasparra, Jennifer ^a,b   Gouraud, Aurore ^a,b   Mougin, Bruno ^a,b   Miossec, Pierre ^a,b  

^a EDOUARD HERRIOT HOSPITAL   (France)

^b BIOMÉRIEUX   (France)

Author keywords

DNA extraction; Dried blood spots; Genotyping; Shared epitope

Indexed keywords

CHLOROFORM; EDETIC ACID; EPITOPE; GENOMIC DNA; HLA DR ANTIGEN; PHENOL;

ADULT; AGED; AMPLICON; ARTICLE; AUTOMATION; BLOOD STAIN; CLINICAL PRACTICE; DNA EXTRACTION; FEMALE; GENETIC POLYMORPHISM; GENOTYPE; HUMAN; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHEUMATOID ARTHRITIS; TURNAROUND TIME;

ARTHRITIS, RHEUMATOID; BLOOD SPECIMEN COLLECTION; DNA; EPITOPES; FEMALE; HEMATOLOGIC TESTS; HLA-DR ANTIGENS; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; REPRODUCIBILITY OF RESULTS; TIME;

EID: 35548979546     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.08.001     Document Type: Article

References (18)

1. Cros P., Allibert P., Mandrand B., Tiercy J.M., and Mach B. Oligonucleotide genotyping of HLA polymorphism on microtitre plates. Lancet 340 (1992) 870
2. Devost N.C., and Choy F.Y. Mutation analysis of Gaucher disease using dot-blood samples on FTA filter paper. Am. J. Med. Genet. 94 (2000) 417
3. Firestein G.S. Evolving concepts of rheumatoid arthritis. Nature 423 (2003) 356
4. Gregersen P.K., Silver J., and Winchester R.J. The shared epitope hypothesis. An approach to understanding the molecular genetics of susceptibility to rheumatoid arthritis. Arthritis. Rheum. 30 (1987) 1205
5. Kline M.C., Duewer D.L., Redman J.W., Butler J.M., and Boyer D.A. Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival media. Anal. Chem. 74 (2002) 1863
6. Ledray L.E., and Netzel L. DNA evidence collection. J. Emerg. Nurs. 23 (1997) 156
7. Marotte H., Pallot-Prades B., Grange L., Tebib J., Gaudin P., Alexandre C., Blond J.L., Cazalis M.A., Mougin B., and Miossec P. The shared epitope is a marker of severity associated with selection for, but not with response to, infliximab in a large rheumatoid arthritis population. Ann. Rheum. Dis. 65 (2006) 342
8. Mougin B., Garnero P., Borel O., Compagnon C., Barbalat V., Marotte H., and Miossec P. A routine assay for the direct analysis of HLA-DR-related shared epitope and B27 alleles in chronic inflammatory arthritis. J. Immunol. Methods, 256 (2001) 47
9. Nepom G.T. Major histocompatibility complex-directed susceptibility to rheumatoid arthritis. Adv. Immunol. 68 (1998) 315
10. O'Dell J.R., Nepom B.S., Haire C., Gersuk V.H., Gaur L., Moore G.F., Drymalski W., Palmer W., Eckhoff P.J., Klassen L.W., Wees S., Thiele G., and Nepom G.T. HLA-DRB1 typing in rheumatoid arthritis: predicting response to specific treatments. Ann. Rheum. Dis. 57 (1998) 209
11. Renshaw M., Ellingsen D., Benson J., and Hooper W.C. Amplification of DNA from blood spots: the importance of collection paper and primer sequence. Focus 23 (2001) 8
12. Reveille J.D. The genetic contribution to the pathogenesis of rheumatoid arthritis. Curr. Opin. Rheumatol. 10 (1998) 187
13. Tang Y.W., Sefers S.E., Li H., Kohn D.J., and Procop G.W. Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens. J. Clin. Microbiol. 43 (2005) 4830
14. van der Helm-van Mil A.H., Wesoly J.Z., and Huizinga T.W. Understanding the genetic contribution to rheumatoid arthritis. Curr. Opin. Rheumatol. 17 (2005) 299
15. van Deursen P., de Bie P., Verhoeven A., van Grinsven A., Jacobs M., Borst G., and van de Wiel P. Nucleic acid isolation using magnetic silica particles. Second European Meeting on Molecular Diagnostics (2003) 2003
16. *1 allele, possibly due to salivary acetaldehyde. Gut 53 (2004) 871
17. Weyand C.M., Hicok K.C., Conn D.L., and Goronzy J.J. The influence of HLA-DRB1 genes on disease severity in rheumatoid arthritis. Ann. Intern. Med. 117 (1992) 801
18. Wordsworth P., Pile K.D., Buckely J.D., Lanchbury J.S., Ollier B., Lathrop M., and Bell J.I. HLA heterozygosity contributes to susceptibility to rheumatoid arthritis. Am. J. Hum. Genet. 51 (1992) 585