Detection and identification of bacterial enteropathogens by polymerase chain reaction and conventional techniques in childhood acute gastroenteritis in Gaza, Palestine

International Journal of Infectious Diseases

Volumn 11, Issue 6, 2007, Pages 501-507

  Abu Elamreen, Farid H ^a   Abed, Abdalla A ^b   Sharif, Fadel A ^b  

^a MINISTRY OF HEALTH   (Canada)

^b ISLAMIC UNIVERSITY OF GAZA   (Palestine)

Author keywords

Bacterial gastroenteritis; Diarrhea; Enteropathogens; Gaza; Palestine; Polymerase chain reaction (PCR)

Indexed keywords

AMPICILLIN; ANTIINFECTIVE AGENT; CEFACLOR; CEFALEXIN; CEFTAZIDIME; CEFUROXIME; CHLORAMPHENICOL; COTRIMOXAZOLE; DOXYCYCLINE; MEROPENEM; NALIDIXIC ACID;

ACUTE GASTROENTERITIS; AMINO ACID SEQUENCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL INFECTION; BACTERIUM DETECTION; BACTERIUM ISOLATION; CAMPYLOBACTER; CHILD; CONTROLLED STUDY; ENTEROPATHY; ESCHERICHIA COLI; HUMAN; MAJOR CLINICAL STUDY; PALESTINE; POLYMERASE CHAIN REACTION; SALMONELLA; SHIGELLA;

ANTI-BACTERIAL AGENTS; BACTERIA; BACTERIAL INFECTIONS; CHILD, PRESCHOOL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GASTROENTERITIS; HUMANS; INFANT; MIDDLE EAST; POLYMERASE CHAIN REACTION;

EID: 35448977773     PISSN: 12019712     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijid.2007.01.010     Document Type: Article

References (34)

1. Kosek M., Bern C., and Guerrant R.L. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 81 (2003) 197-204
2. Nelson E., Ko W., Kwan E., Leung F., Poon H., Chow B., et al. Guidelines for the management of acute diarrhoea in young children. HK J Paediatr 8 (2003) 203-236
3. Awad R., and Abu Arquob O. The status of health in Palestine (2001), HMIS-MOH
4. Armon K., and Elliott E. Acute gastroenteritis. In: Moyer V.A., Elliott E.J., Davis R.L., Gilbert R., Klassen T., Logan S., et al. (Eds). Evidence-based pediatrics and child health (2000), BMJ Books, London 273-286
5. Abu Mourad T. Palestinian refugee conditions associated with intestinal parasites and diarrhoea: Nuseirat refugee camp as a case study. Public Health 118 (2004) 131-142
6. Sallon S., el-Shawwa R., Khalil M., Ginsburg G., el Tayib J., el-Eila J., et al. Diarrhoeal disease in children in Gaza. Ann Trop Med Parasitol 88 (1994) 175-182
7. Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Shapiro C., et al. Food-related illness and death in the United States. Emerg Infect Dis 5 (1999) 607-625
8. American Medical Association; Centers for Disease Control and Prevention; Center for Food Safety and Applied Nutrition, Food and Drug Administration; Food Safety and Inspection Service, US Department of Agriculture. Diagnosis and management of foodborne illnesses: a primer for physicians. MMWR Recomm Rep 50 RR-2 (2001) 1-69
9. Ministry of Health. Health status in Palestine-The annual report 2004 (2005), Health Information Center, Gaza, Palestine
10. Ministry of Health. Health status in Palestine-The annual report 2002 (2003), Health Information Center, Gaza, Palestine
11. Ministry of Health. Health status in Palestine-The annual report 2003 (2004), Health Information Center, Gaza, Palestine
12. Babalola O. Molecular techniques: an overview of methods for the detection of bacteria. Afric J Biotechnol 2 (2003) 710-713
13. Islam M., Hossain M., Hasan M., Rahman M., Fuchs G., Mahalanabis D., et al. Detection of Shigellae from stools of dysentery patients by culture and polymerase chain reaction techniques. J Diarrhoeal Dis Res 16 (1998) 248-251
14. Kulkarni S.P., Lever S., Logan J.M., Lawson A.J., Stanley J., and Shafi M.S. Detection of Campylobacter species: a comparison of culture and polymerase chain reaction based methods. J Clin Pathol 55 (2002) 749-753
15. Lampel K., Orlandi P., and Kornegay L. Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens. Appl Environ Microbiol 66 (2000) 4539-4542
16. World Health Organization. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health concern in the developing world. Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae, Neisseria gonorrhoeae, Salmonella serotype Typhi, Shigella, and Vibrio cholerae. WHO/CDS/CSR/RMD/2003.6. Available at: http://www.who.int/csr/resources/publications/drugresist/WHO_CDS_CSR_RMD_2003_6/en/ (accessed February 2007).
17. Vandepitte J., Verhaegen J., Engbaek K., Rohner P., Piot P., and Heuck C. Basic laboratory procedures in clinical microbiology. 2nd ed. (2003), World Health Organization, Geneva
18. Paton J., and Paton A.W. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev 11 (1998) 450-479
19. Isenberg H. Essential procedures for clinical microbiology (1998), ASM Press, Washington DC
20. World Health Organization. Guidelines on standard operating procedures for microbiology, 2005. SEA/HLM/324. Geneva: World Health Organization; 2005.
21. Ziemer C.J., and Steadham S.R. Evaluation of the specificity of Salmonella PCR primers using various intestinal bacterial species. Lett Appl Microbiol 37 (2003) 463-469
22. Cebula T.A., Payne W.L., and Feng P. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol 33 (1995) 248-250
23. Linton D., Lawson J., Owen R.J., and Stanley J. PCR detection and identification to species level and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J Clin Microbiol 35 (1997) 2568-2572
24. Tjaniadi P., Lesmana M., Subekti D., Machpud N., Komalarini S., Santoso W., et al. Antimicrobial resistance of bacterial pathogens associated with diarrheal patients in Indonesia. Am J Trop Med Hyg 68 (2003) 666-670
25. Wasfy M.O., Oyofo B.A., David J.C., Ismail T.F., el-Gendy A.M., Mohran Z.S., et al. Isolation and antibiotic susceptibility of Salmonella, Shigella, and Campylobacter from acute enteric infections in Egypt. J Health Popul Nutr 18 (2000) 33-38
26. Riley L.W., Remis R.S., Helgerson S.D., McGee H.B., Wells J.G., Davis B.R., et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med 308 (1983) 681-685
27. Sharif F, Arafa H. Occurrence of Escherichia coli O157 in Gaza Strip: a preliminary study. Journal of the Islamic University of Gaza (natural sciences series), 2004;12:51-6.
28. Hunt JM, Abeyta C, Tran T. Bacteriological analytical manual. 8th ed. Revision A, chapter 7. US Food and Drug Administration; 1998. Available at: http://www.cfsan.fda.gov/∼ebam/bam-toc.html (accessed February 2007).
29. Samuel B. Medical microbiology (1996), The University of Texas Medical Branch, Galveston, Texas USA
30. Materu S.F., Lema O.E., Mukunza H.M., Adhiambo C.G., and Carter J.Y. Antibiotic resistance patterns of Vibrio cholerae and Shigella causing diarrhoea outbreaks in the eastern African region: 1994-1996. East Afr Med J 74 (1997) 193-197
31. Nguyen V. Children's diarrhea in Hanoi, Vietnam: importance of enteric pathogens (2005), Karolinska University Press, Stockholm, Sweden
32. Subekti D., Oyofo B.A., Tjaniadi P., Corwin A.L., Larasati W., Putri M., et al. Shigella spp. surveillance in Indonesia: the emergence or re-emergence of S. dysenteriae. Emerg Infect Dis 7 (2001) 137-140
33. Ashkenazi S., May-Zahav M., Sulkes J., Zilberberg R., and Samra Z. Increasing antimicrobial resistance of Shigella isolates in Israel during the period 1984 to 1992. Antimicrob Agents Chemother 39 (1995) 819-823
34. Chu Y.W., Houang E.T., Lyon D.J., Ling J.M., Ng T.K., and Cheng A.F. Antimicrobial resistance in Shigella flexneri and Shigella sonnei in Hong Kong, 1986 to 1995. Antimicrob Agents Chemother 42 (1998) 440-443